In vitro determination of the efficacy of scorpion venoms as anti-cancer agents against colorectal cancer cells: a nano-liposomal delivery approach

Alasmari, Abdulrahman; Ullah, Zabih; Balowi, Ali Al; Islam, Mohammad Mahmudul

doi:10.2147/ijn.s123514

Cited by 20 publications

(8 citation statements)

References 44 publications

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“…For this reason, researchers are working towards target therapy for the development of the most effective treatment. For decades, scorpion venom has been used in traditional treatments, and recently researchers exhibited the anticancer properties of scorpion venom 26 . In this study, we examined the cytotoxic effect of the crude scorpion venoms, including Androctonus Crassicauda and Hottentotta Saulcyi on the CRC cell line (HT-29).…”

Section: Discussionmentioning

confidence: 99%

In vitro anticancer activity of hydrogen sulfide and nitric oxide alongside nickel nanoparticle and novel mutations in their genes in CRC patients

Housein

Kareem

Salihi

2021

Sci Rep

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This study was carried out to assess the impact of nickel nanoparticles (NiNPs) as well as scorpion venom on colorectal cancer (CRC) cells in the presence and/or absence of 5-fluorouracil (5-FU), hydrogen sulfide (H2S), and nitric oxide (NO) donors and to determine alterations in endothelial NO synthase (eNOS) and cystathionine γ-lyase (CSE) enzyme-producing genes in CRC patients. The IC50 of both H2S and NO donors, along with NiNPs, were determined. The CRC cells were treated for 24hrs, and the cytotoxic activities were assessed using the MTT test. Moreover, the apoptosis was determined after 24hrs and 48hrs using TUNEL assay. Furthermore, the mutations in the eNOS gene (intron 4, -786T>C and 894 G>T) and CSE gene (1364GT) were determined using direct sequencing. The IC50 values for sodium disulfide (Na2S) and sodium nitroprusside (SNP) at 24hrs treatment were found to be 5 mM and 10−6 M, respectively, while the IC50 value for 5-FU was reached after 5-days of treatment in CRC cell line. Both black and yellow scorpion venoms showed no inhibition of cell proliferation after 24hrs treatment. Furthermore, Na2S showed a significant decrease in cell proliferation and an increase in apoptosis. Moreover, a co-treatment of SNP and 5-FU resulted in inhibition of the cytotoxic effect of 5-FU, while a combination treatment of NiNPs with Na2S, SNP, and 5-FU caused highly significant cytotoxicity. Direct sequencing reveals new mutations, mainly intronic variation in eNOS gene that has not previously been described in the database. These findings indicate that H2S promotes the anticancer efficiency of 5-FU in the presence of NiNPs while NO has antiapoptotic activity in CRC cell lines.

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Section: Discussionmentioning

confidence: 99%

In vitro anticancer activity of hydrogen sulfide and nitric oxide alongside nickel nanoparticle and novel mutations in their genes in CRC patients

Housein

Kareem

Salihi

2021

Sci Rep

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“…It has been reported that scorpion venom can induce apoptosis and has a signicant anti-tumor effect in vitro. 21,22 In order to investigate the apoptosis-inducing effect of MCLO-12, ow cytometry using propidium iodide (PI) and Annexin-V in A549 cells was performed. Aer incubation with the MCLO-12 described above for 48 h, the cells were stained with Annexin V/PI, and the percentage of apoptotic cells was measured using a ow cytometer.…”

Section: Mclo-12 Induces Apoptosismentioning

confidence: 99%

Structural characterization of Momordica charantia L. (Cucurbitaceae) oligopeptides and the detection of their capability in non-small cell lung cancer A549 cells: induction of apoptosis

Dong

Zhang

et al. 2019

RSC Adv.

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“…Human breast cancer cell line MDA-MB-231 (obtained from breast mammary glands) and colorectal cancer cell line HCT-8 (obtained from the ileocecal adenocarcinoma of a 67-year-old male) were cultured in Dulbecco's Modified Eagle Medium supplemented with antibiotic-anti-mycotic as reported earlier. 20,21 MTT Colorimetric Assay to Determine the Cytotoxic Effect of the Venoms…”

Section: Cell Culturementioning

confidence: 99%

Scorpion Venom Causes Apoptosis by Increasing Reactive Oxygen Species and Cell Cycle Arrest in MDA-MB-231 and HCT-8 Cancer Cell Lines

Alasmari

Riyasdeen

Islam

2018

J Evid Based Complementary Altern Med

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Scorpion venom induces apoptosis in breast and colorectal cell lines as reflected by the changes in the cell morphology and cell cycle studies. Furthermore, a high percentage of total reactive oxygen species as well as apoptotic cells also contribute to cell death as observed after venom treatments. To the best of authors' knowledge, this is the first scientific evidence demonstrating the induction of apoptosis and cell cycle arrest by these species of scorpion venoms.

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In vitro determination of the efficacy of scorpion venoms as anti-cancer agents against colorectal cancer cells: a nano-liposomal delivery approach

Cited by 20 publications

References 44 publications

In vitro anticancer activity of hydrogen sulfide and nitric oxide alongside nickel nanoparticle and novel mutations in their genes in CRC patients

In vitro anticancer activity of hydrogen sulfide and nitric oxide alongside nickel nanoparticle and novel mutations in their genes in CRC patients

Structural characterization of Momordica charantia L. (Cucurbitaceae) oligopeptides and the detection of their capability in non-small cell lung cancer A549 cells: induction of apoptosis

Scorpion Venom Causes Apoptosis by Increasing Reactive Oxygen Species and Cell Cycle Arrest in MDA-MB-231 and HCT-8 Cancer Cell Lines

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