In vitro development and chromosome constitution of embryos derived from monopronucleated zygotes after intracytoplasmic sperm injection

Mateo, Silvia María; Parriego, M; Boada, Montserrat; Vidal, Francesca; Coroleu, Buenaventura; Veiga, Anna

doi:10.1016/j.fertnstert.2012.11.014

Cited by 42 publications

(52 citation statements)

References 15 publications

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“…Sultan et al, reported that Y‐chromosome prevalence in 1PN was low (10%), and in most cases, the development was parthenogenetic. Furthermore, chromosomal aberrations were found in all analyzed cases of 1PN obtained by ICSI. Levron et al, suggested that 1PN embryos obtained by IVF are possibly formed through enclosure of the paternal and maternal chromosomes by a single nuclear membrane.…”

Section: Discussionmentioning

confidence: 99%

Clinical outcomes of transfer of frozen and thawed single blastocysts derived from nonpronuclear and monopronuclear zygotes

Hondo¹,

Arichi²,

Muramatsu³

et al. 2019

Reprod Medicine & Biology

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Purpose In assisted reproductive technology, normal zygotes are bipronuclear (2PN) during fertilization confirmation; however, sometimes, nonpronuclear zygotes (0PN) and monopronuclear zygotes (1PN) are found during routine observations. Methods To elucidate the clinical usefulness of in vitro‐fertilized embryos, we investigated the rates of clinical pregnancy, live birth, miscarriage, and congenital abnormality after transfer of frozen‐thawed 1PN‐ and 0PN‐derived single blastocysts at Denentoshi Ladies Clinic, Kanagawa, Japan. Results The rates of pregnancy and live birth for 1PN‐derived blastocysts obtained by conventional in vitro fertilization were 37.5% and 27.1%, respectively, which was not significantly different from those for 2PN‐derived blastocysts; however, the rates for 0PN‐derived blastocysts were significantly lower. The pregnancy and live birth rates for 0PN‐derived embryos obtained by intracytoplasmic sperm injection (ICSI) were 45.7% and 34.8%, respectively, which was not significantly different from those for 2PN‐derived blastocysts; however, the rates for 1PN‐derived blastocysts were significantly lower (4.0% for both) than those for 2PN‐ and 0PN‐derived blastocysts. No congenital abnormalities were found in infants resulting from transfer of 0PN‐ or 1PN‐derived blastocysts. Conclusions Both 1PN‐ and 0PN‐derived blastocysts can be used for embryo transfer; however, care should be taken in making decisions about 1PN‐derived blastocysts, especially if they are obtained by ICSI.

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Section: Discussionmentioning

confidence: 99%

Clinical outcomes of transfer of frozen and thawed single blastocysts derived from nonpronuclear and monopronuclear zygotes

Hondo¹,

Arichi²,

Muramatsu³

et al. 2019

Reprod Medicine & Biology

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“…Otsu et al also reported that all analyzed 1PN blastocysts in cIVF were diploid [20]. However, the diploid rate of ICSI 1PN blastocysts was 37.5 % [19]. van der Heijden et al reported that 86.7 % of the 1PN zygotes included the maternal and paternal genome in cIVF and that the diploid rate of cIVF cycles was higher than that of ICSI cycles (30.3 %) using immunostaining based on histon modification [11].…”

Section: Discussionmentioning

confidence: 99%

“…Several attempts to analyze human 1PN zygotes and embryos in cIVF and ICSI cycles have been reported, such as the mechanism of 1PN formation on fertilization [4,6,[16][17][18]; chromosome constitution in zygote, embryo, and blastocyst stages [6,9,10,12,[19][20][21]; and genetic composition of the pronucleus in human [11] and mouse [22]. According to these data, when the chromosomal status of 1PN embryos was analyzed using fluorescence in situ hybridization (FISH), the rate of diploid chromosome constitution in cIVF was significantly higher than that in ICSI [6,9,10].…”

Section: Discussionmentioning

confidence: 99%

“…van der Heijden et al reported that 86.7 % of the 1PN zygotes included the maternal and paternal genome in cIVF and that the diploid rate of cIVF cycles was higher than that of ICSI cycles (30.3 %) using immunostaining based on histon modification [11]. Moreover, Mateo et al reported that when 1PN embryos were cultured to blastocyst in ICSI, the blastocyst formation and good-quality blastocyst rates were only 14.8 and 7.4 %, respectively [19]. In addition, Reichman et al reported that the implantation rate and pregnancy rates of the 1PN embryos from cIVF or ICSI were only 6.4 and 1.3 %, respectively [5].…”

Section: Discussionmentioning

confidence: 99%

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Birth of nine normal healthy babies following transfer of blastocysts derived from human single-pronucleate zygotes

Itoi¹,

Asano²,

Shimizu³

et al. 2015

J Assist Reprod Genet

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Purpose The purpose of this study is to examine the clinical outcomes of blastocysts derived from human singlepronucleate (1PN) embryos after conventional in vitro fertilization (cIVF) and intracytoplasmic sperm injection (ICSI) cycles. Methods This was a retrospective study at a reproductive center of a hospital. To evaluate embryo quality and clinical outcomes, cIVF or ICSI cycles with one or more 1PN embryos were compared with same cycles with 2PN embryos (control cycles).Results A total of 623 cycles (426 cIVF cycles and 197 ICSI cycles) were treated with cIVF or ICSI. The single pronuclear status rate was similar between cIVF (22.1 %) and ICSI (25.1 %) cycles. Although the development rates of 1PN embryos on day 3 and day 5/6 in cIVF were significantly higher than those in ICSI, those of 1PN embryos in cIVF were significantly lower compared to 2PN embryos (p<0.01). Nonetheless, the ongoing pregnancy rates achieved with 1PN blastocysts in 1PN embryos did not significantly differ from the control group. Thirty-three transfer cycles with 33 blastocysts derived from 1PN embryos in cIVF resulted in nine deliveries with no newborn malformations; however, no implantation was observed in three ICSI cycles. Conclusion Although the blastocyst formation rate of 1PN embryos was significantly lower than 2PN embryos in cIVF and ICSI cycles, 1PN blastocysts in cIVF, and not from ICSI, demonstrated an adequate ongoing pregnancy rate. These results suggested that 1PN blastocysts in cIVF are available for clinical use and may lead to an increase in the chance of pregnancy in patients receiving assisted reproductive technology with 1PN embryos.

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“…analysis: (IVF: 80.5% diploid, cytogenetics), 1 (IVF: 71.4% diploid; ICSI: 28.6% diploid; FISH), 9 (ICSI: none diploid; cytogenetics), 10 (ICSI: none diploid; FISH) 15 ; but not consistent with other previous results from zygotes: (IVF: 58% diploid; cytogenetics, FISH), 13 (IVF: 37.5% diploid; FISH) 16 and embryos: (IVF: 47.6% diploid; PCR and FISH), 7 (IVF: 48.7% diploid; ICSI: 27.9% diploid; FISH). 11 To date, three published reports have described the birth of 5 healthy children from IVF treatment cycles, with embryos derived from 1 PN/2 PB zygotes as no other embryos were available for transfer.…”

Section: Introductionmentioning

confidence: 99%

Molecular Cytogenetics of Human Single Pronucleated Zygotes

et al. 2014

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The aim of the present study was to use fluorescence in situ hybridization to analyze the chromosome status of zygotes with a single pronucleus from in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) treatment cycles. In addition, we performed immunocytochemical detection of nuclear lamins and histone H3 trimethylated at lysine-9, Me(3)H3K9. Zygotes were processed 24 hours after insemination or injection to assure the absence of asynchrony. In opposition to previous results, we observed 2 pronuclei in 16 of 18 IVF zygotes and 40 of 64 ICSI zygotes, suggesting premature pronuclear breakdown. In IVF and ICSI zygotes, the rate of normal diploidy was only 6 of 16 and 27 of 56, respectively, suggesting that monopronucleated zygotes should not be used in assisted reproductive treatments. The possible mechanisms are discussed and compared to previous studies of monopronucleated zygotes.

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In vitro development and chromosome constitution of embryos derived from monopronucleated zygotes after intracytoplasmic sperm injection

Cited by 42 publications

References 15 publications

Clinical outcomes of transfer of frozen and thawed single blastocysts derived from nonpronuclear and monopronuclear zygotes

Clinical outcomes of transfer of frozen and thawed single blastocysts derived from nonpronuclear and monopronuclear zygotes

Birth of nine normal healthy babies following transfer of blastocysts derived from human single-pronucleate zygotes

Molecular Cytogenetics of Human Single Pronucleated Zygotes

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