In vitro development and preservation of specific features of collecting duct epithelial cells from embryonic rabbit kidney are regulated by the electrolyte environment

Steiner, Pat; Strehl, Raimund; Kloth, Sabine; Tauc, Michel; Minuth, Will W.

doi:10.1046/j.1432-0436.1998.6240193.x

Cited by 18 publications

(17 citation statements)

References 33 publications

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“…Renal CD epithelia were generated from embryonic CD ampulla cells on a kidney-specific collagenous support. During perfusion culture, a polarized CD epithelium was established, which in analogy to the adult kidney did not show any more mitotic cells ( Figure 3E) (19). Applying physiologic concentrations of urea over 14 d, we analyzed in the present experiments the histochemical profile of generated CD epithelia.…”

Section: Discussionmentioning

confidence: 94%

“…It was shown in earlier investigations that addition of NaCl to IMDM modulates differentiation of embryonic CD epithelia (19). Consequently, 20 mmol/L NaCl was added to IMDM to adapt the serum level for Na ϩ in the culture medium (Figure 6; n ϭ 7 in each group).…”

Section: Culture Of Embryonic CD Epithelia In Imdm Containing Aldostementioning

confidence: 91%

“…It has been shown that alkali feeding of pregnant mothers triggers an earlier appearance of ␤-type IC cells compared with controls (18). Furthermore, it was demonstrated that the electrolyte environment of cultured embryonic CD epithelia is able to influence the development of P and IC cell features (19). Experiments with a tissue-specific model additionally showed that application of aldosterone in the culture medium increased the amount of PNA-positive cells in maturing CD epithelia (20).…”

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confidence: 99%

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Urea Restrains Aldosterone-Induced Development of Peanut Agglutinin–Binding on Embryonic Renal Collecting Duct Epithelia

Schumacher¹,

Strehl²,

Minuth³

2003

Journal of the American Society of Nephrology

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Abstract. Peanut agglutinin (PNA) represents a commonly used marker for ␤-type intercalated (IC) cells and their distribution in the corticomedullary course of the collecting duct (CD) in the mature rabbit kidney. It has been shown that aldosterone is able to generate Ͼ90% of PNA-binding cells in an embryonic CD epithelium in vitro. In adult kidney, a maximum of only 25% PNA-positive cells is found in the cortical segment of the CD, and PNA-binding completely disappears in the inner-medullary CD. Molecules that regulate the gradual development of CD-specific cells during organ growth are unknown. In the present experiments, it was found that addition of physiologic concentrations of urea to the culture medium is able to restrain the action of aldosterone in embryonic CD epithelia. Urea antagonizes in a concentration-dependent manner the action of aldosterone finally leading to only 10% of PNA-binding cells. The data point to a urea-specific effect, because osmolytes such as NaCl and mannitol did not affect PNA binding. In addition, urea did not influence expression of principal-cell typical markers such as AQP2 and 3. The findings may explain that a higher number of PNA-positive cells is found in the cortical region of the kidney correlated with a low concentration of urea as compared with only few PNA-binding cells in the medullary CD, where a high concentration of urea occurs. Thus, an increasing concentration of urea may trigger the number of PNA-positive cells in the cortical-medullary course of the CD during organ development.

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Section: Discussionmentioning

confidence: 94%

Section: Culture Of Embryonic CD Epithelia In Imdm Containing Aldostementioning

confidence: 91%

mentioning

confidence: 99%

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Urea Restrains Aldosterone-Induced Development of Peanut Agglutinin–Binding on Embryonic Renal Collecting Duct Epithelia

Schumacher¹,

Strehl²,

Minuth³

2003

Journal of the American Society of Nephrology

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“…Application of a gradient perfusion culture container made it further possible to investigate the influence on differentiation of different fluid composition at the luminal and basal sides of embryonic renal collecting duct (CD) epithelia (Minuth et al 1992b(Minuth et al , 1997a(Minuth et al , b, 1999(Minuth et al , 2001(Minuth et al , 2005aSteiner et al 1997, Schumacher et al 2002aMinuth et al 2009a). In the course of performed experiments it was detected that development of a CD epithelium starts with an unexpected long latent period of three days and needs at least 10 days for up-regulation of typical signs of differentiation.…”

Section: Featuring Development Of Epitheliamentioning

confidence: 99%

Bridging the gap between traditional cell cultures and bioreactors applied in regenerative medicine: practical experiences with the MINUSHEET perfusion culture system

Minuth

Denk

2015

Cytotechnology

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To meet specific requirements of developing tissues urgently needed in tissue engineering, biomaterial research and drug toxicity testing, a versatile perfusion culture system was developed. First an individual biomaterial is selected and then mounted in a MINUSHEET Ò tissue carrier. After sterilization the assembly is transferred by fine forceps to a 24 well culture plate for seeding cells or mounting tissue on it. To support spatial (3D) development a carrier can be placed in various types of perfusion culture containers. In the basic version a constant flow of culture medium provides contained tissue with always fresh nutrition and respiratory gas. For example, epithelia can be transferred to a gradient container, where they are exposed to different fluids at the luminal and basal side. To observe development of tissue under the microscope, in a different type of container a transparent lid and base are integrated. Finally, stem/progenitor cells are incubated in a container filled by an artificial interstitium to support spatial development. In the past years the described system was applied in numerous own and external investigations. To present an actual overview of resulting experimental data, the present paper was written.

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“…Recent data from in vitro experiments indicate that two very different cell biological mechanisms can influence terminal differentiation of individual CD cell populations: the humoral action of aldosterone and changes in the eletrolyte composition are under discussion [7][8][9][10][11]. Culture experiments in the presence of fetal calf serum and in the stagnant environment of a culture dish have shown that aldosterone acts as a differentiation hormone on embryonic CD cells [12].…”

Section: Introductionmentioning

confidence: 99%

Embryonic Renal Collecting Duct Cell Differentiation Is Influenced in a Concentration-Dependent Manner by the Electrolyte Environment

et al. 2001

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Background: During kidney development, the embryonic collecting duct (CD) epithelium develops into a heterogeneously composed epithelium consisting of principal and intercalated cells. It is unknown by which molecular mechanism the different cell types arise. We have experimental evidence that the electrolyte environment is involved in the process of terminal cell differentiation. Methods: Embryonic CD epithelia from neonatal rabbit kidneys were microsurgically isolated and maintained in gradient perfusion culture for 13 days under serum-free conditions. Controls were maintained in the same medium (Iscove’s modified Dulbecco’s medium; IMDM) on basal and luminal sides. Experimental series were performed with IMDM only on the basal side, while on the luminal side IMDM with increasing concentrations of NaCl was used. Finally, the development of principal and intercalated cell features was registered by immunohistochemical labeling with markers specific for adult CD cells. Results: Immunohistochemical markers show that the differentiation pattern is quite different when the embryonic CD epithelia are cultured in IMDM only as compared with specimens kept in IMDM supplemented with 3–24 mmol/l NaCl on the luminal cell side. First signs of changes in development were seen when low doses of 3–6 mmol/l NaCl were added. Conclusions: We conclude that facultative protein expression in embryonic CD epithelium is influenced by the electrolyte environment and starts to be upregulated after administration of unexpectedly low doses of 3–6 mmol/l NaCl added to the luminal perfusion culture medium and increases in a concentration-dependent manner.

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In vitro development and preservation of specific features of collecting duct epithelial cells from embryonic rabbit kidney are regulated by the electrolyte environment

Cited by 18 publications

References 33 publications

Urea Restrains Aldosterone-Induced Development of Peanut Agglutinin–Binding on Embryonic Renal Collecting Duct Epithelia

Urea Restrains Aldosterone-Induced Development of Peanut Agglutinin–Binding on Embryonic Renal Collecting Duct Epithelia

Bridging the gap between traditional cell cultures and bioreactors applied in regenerative medicine: practical experiences with the MINUSHEET perfusion culture system

Embryonic Renal Collecting Duct Cell Differentiation Is Influenced in a Concentration-Dependent Manner by the Electrolyte Environment

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