In vitro development of core cells of the inner cell mass of the mouse blastocyst: Effects of conditioned medium

Atienza-Samols, Sui Bi; Sherman, Michael I.

doi:10.1002/jez.1402080108

Cited by 9 publications

(2 citation statements)

References 7 publications

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“…Although the dissection of the pig ICM into its component epiblast and endoderm portions showed both to contain AP-positive cells, the outermost layers of the endoderm were not stained, whereas the epiblast core appeared to be stained uniformly. Dissection to isolate the epiblast core from mouse ICMs has been done, but these reports did not mention the AP profile of the parts (Hagan and Tilly, 1977;Pedersen et al, 1977;Atienza-Samols and Sherman, 1979).…”

Section: Discussionmentioning

confidence: 99%

Alkaline phosphatase staining of pig and sheep epiblast cells in culture

Talbot

Rexroad

Pursel

et al. 1993

Molecular Reproduction Devel

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To define better the characteristics of pig and sheep epiblast cells in culture, the cells were tested for the presence of alkaline phosphatase (AP), a biochemical marker characteristic of mouse embryonic stem cells. Pig and sheep epiblast cells were positive for AP staining both at isolation from the blastocyst and after primary in vitro culture. The innermost portion of the attendant endoderm surrounding the epiblast was also positive for AP staining during primary culture. AP staining was lost upon differentiation or senescence of the epiblast cells. Also, all differentiated epiblast-derived cell cultures were negative for AP staining, with the exception of neuron-like cultures. Epiblast-like cells were cultured from day 10 (pig) and day 13 (sheep) embryonic discs, and these cells were also AP positive until they differentiated. Trophectoderm-endoderm-like cells from embryonic discs were AP negative or weakly positive. AP is a convenient marker for undifferentiated pig and sheep epiblast cells in culture when used in conjunction with cell morphology analysis.

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Section: Discussionmentioning

confidence: 99%

Alkaline phosphatase staining of pig and sheep epiblast cells in culture

Talbot

Rexroad

Pursel

et al. 1993

Molecular Reproduction Devel

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“…In some cases, embryoid bodies form with no evidence of trophoblast outgrowth (Solter and Knowles 1975;Wiley et al 1978;Hogan and Tilly 1978a;Surani et al 1978). In other cases, trophoblast giant cells have developed from ICMs (Atienza-Samols and Sherman 1978), from clumps of dissociated ICM cells Tilly 1978a, 1978b) and from ICM 'cores' (Wiley 1978;Atienza-Samols and Sherman 1979). These studies suggest that some or all of the ICM cells are capable of altering their development in response to environmental changes till quite late in development.…”

Section: Introductionmentioning

confidence: 96%

Properties of Mouse Inner Cell Masses Isolated by Immunosurgery, Exposure to the Calcium Ionophore A23187 or Low Osmolarity

Harlow¹,

Quinn²

1980

Aust. Jnl. Of Bio. Sci.

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Mouse inner cell masses remained intact when exposed to extreme bsmotic stress (distilled water) for short periods, but the trophectoderm was lysed in one-third of blastocysts. However, these inner cell masses were not viable as they could not fluoresce after incubation in fluorescein diacetate nor continue development in vitro. It was concluded that cells of the inner cell mass are not more tolerant of osmotic stresses than those of the trophectoderm.Inner cell masses were isolated from only 50 % of blastocysts when exposed to the calcium ionophore A23187. Some lysing trophectoderm cells are probably able to restore their normal calcium levels after transfer to fresh culture medium allowing normal blastocyst-like development in vitro. Further evidence which suggests that not all trophectoderm cells are removed after incubation in ionophore is that these inner cell masses produced more trophoblast-like outgrowths in vitro and were able to induce the decidual cell reaction in vivo more often than their immunosurgically isolated counterparts.Irnmunosurgically isolated inner cell masses were viable as they fluoresced brightly after incubation in fluorescein diacetate and developed normally in vitro. There was little or no contamination of these inner cell masses with trophectoderm cells as they formed considerably smaller outgrowths than control blastocysts and were rarely able to induce the decidual cell reaction in vivo.Trophoblast-like giant cells were found less frequently from inner cell masses isolated from 142-h, post-HCG blastocysts and cultured in vitro, than from li8-h, post-HCG blastocysts. These results are discussed in relation to a current theory on the time of inner cell mass determination which states that apparently differentiated inner cell mass cells may reverse their direction of development in response to altered environmental conditions.

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Cellular and Genetic Analysis of Mouse Blastocyst Development

Pedersen

Spindle

1981

Cellular and Molecular Aspects of Implantation

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In vitro development of core cells of the inner cell mass of the mouse blastocyst: Effects of conditioned medium

Cited by 9 publications

References 7 publications

Alkaline phosphatase staining of pig and sheep epiblast cells in culture

Alkaline phosphatase staining of pig and sheep epiblast cells in culture

Properties of Mouse Inner Cell Masses Isolated by Immunosurgery, Exposure to the Calcium Ionophore A23187 or Low Osmolarity

Cellular and Genetic Analysis of Mouse Blastocyst Development

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