In vitro differentiation of fat-storing cells parallels marked increase of collagen synthesis and secretion

Modulation of Collagen Synthesis and Degradation by Retinoids and Cytokines in 3T3 L1 Preadipocytes.

“…Our preliminary experiments confirmed that 3T3 LI preadipocytes markedly reduced the expression of extracellular matrix proteins and the TGF-B1 gene during the process of differentiation into mature adipocytes (data not shown), as reported previously by another group (1 3). These alterations of gene expressions are similar to those observed in stellate cells in which the expression of extracellular matrix protein and TGF-B1are enhanced in the myofibroblast-like cells while the mature quiescent stellate cells express those genes only faintly (17)(18)(19). Wehave also confirmed that a significant amount of retinoids exist in the lipid droplets of 3T3 LI adipocytes (data not shown) similar to that in stellate cells, since the stellate cells are knownto comprise a major storage site of retinoids in the liver (20).…”

Interferon gamma decreases hepatic stellate cell activation and extracellular matrix deposition in rat liver fibrosis

“…50 Although these techniques cannot discriminate collabecause vitamin A-rich lipid droplets may be dramatically reduced in a variety of conditions, both in vivo 10 gen types, it is well-known that collagen type I is the predominant extracellular matrix protein in cirrhoand in vitro. 34,35 Desmin staining appears as a useful marker for rat HSC under different experimental con-sis. [1][2][3][4] This method has proven, in other studies [67][68] as well as ours, 13 a simple and reproducible technique to ditions, 8,9,19,46 although other markers for normal 63 or activated 36 HSC have recently been proposed.…”

Expression of platelet-derived growth factor-C and insulin-like growth factor I in hepatic stellate cells is inhibited by miR-29

“…17,36 For myofibroblastic transition, HSC were maintained in Dulbecco's modified Eagles medium (DMEM) with 20% fetal calf serum (FCS) the first 2 days after isolation, followed by culture in DMEM with 10 % FCS. Myofibroblastic transition was characterized by induction of a-SMA expression.…”