In vitro Differentiation of Haploid Plants by Anther Culture in Poncirus trifoliata (L.) RAF.

Hidaka, Tetsushi; Yamada, Yoshio; Shichijo, T.

doi:10.1270/jsbbs1951.29.248

Cited by 77 publications

(31 citation statements)

References 10 publications

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“…It was observed that both shoot and root initiation were positively influenced by hormones NAA and BAP, such that the number of roots and shoots per explant increased proportionately with increase in hormone concentration and maximum number of roots and shoots regenerated at a combination of BAP 1 mg/l and NAA 10 mg/l (Usman et al, 2005). Regeneration of Citrus species from pollen has also been reported (Hidaka et al, 1979).…”

Section: Plant Regeneration In Citrusmentioning

confidence: 93%

Citrus biotechnology: Achievements, limitations and future directions

Singh

Rajam

2009

Physiol Mol Biol Plants

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Citrus is one of the most important commercial and nutritional fruit crops in the world, hence it needs to be improved to cater to the diverse needs of consumers and crop breeders. Genetic manipulation through conventional techniques in this genus is invariably a difficult task for plant breeders as it poses various biological limitations comprising long juvenile period, high heterozygosity, sexual incompatibility, nucellar polyembryony and large plant size that greatly hinder cultivar improvement. Hence, several attempts were made to improve Citrus sps. by using various in vitro techniques. Citrus sps are widely known for their recalcitrance to transformation and subsequent rooting, but constant research has led to the establishment of improved protocols to ensure the production of uniformly transformed plants, albeit with relatively low efficiency, depending upon the genotype. Genetic modification through Agrobacterium-mediated transformation has emerged as an important tool for introducing agronomically important genes into Citrus sps. Somatic hybridization has been applied to overcome self and cross-incompatibility barriers and generated inter-specific and inter-generic hybrids. Encouraging results have been achieved through transgenics for resistance against viruses and bacteria, thereby augmenting the yield and quality of the fruit. Now, when major transformation and regeneration protocols have sufficiently been standardized for important cultivars, ongoing citrus research focuses mainly on incorporating such genes in citrus genotypes that can combat different biotic and abiotic stresses. This review summarizes the advances made so far in Citrus biotechnology, and suggests some future directions of research in this fruit crop.

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Section: Plant Regeneration In Citrusmentioning

confidence: 93%

Citrus biotechnology: Achievements, limitations and future directions

Singh

Rajam

2009

Physiol Mol Biol Plants

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“…In Citrus and related genera, some haploid plants have been produced by various techniques such as anther culture (Germanà and Chiancone, 2003;Germanà et al, 1994;Hidaka et al, 1979), interploid hybridization (Germanà and Chiancone, 2001;Oiyama and Kobayashi, 1993;Toolapong et al, 1996), and pollination of irradiated pollen (Aleza et al, 2009;Froelicher et al, 2007;Yahata et al, 2010). However, doubled haploid plants have only been induced by anther culture in Clementine mandarin (C. clementina hort.…”

Section: Introductionmentioning

confidence: 99%

Morphological Characteristics of a Doubled Haploid Line from ‘Banpeiyu’ Pummelo [<i>Citrus maxima</i> (Burm.) Merr.] and Its Reproductive Function

et al. 2015

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To obtain basic information about doubled haploid plants in Citrus, in the present study, we investigated the morphological characteristics in doubled haploid induced by colchicine-treated axillary shoot buds of a haploid plant from 'Banpeiyu' pummelo [C. maxima (Burm.) Merr.]. We also evaluated the reproductive potential of the doubled haploid as a male or a female parent by crossing with some diploids. In term of the results, this doubled haploid had significantly large leaf, flower and fruit compared with those of the original haploid plant. Moreover, the doubled haploid showed higher pollen fertility (84.1% stainability and 32.9% pollen germination rate) and a larger number of seeds (47.2 developed seeds per open-pollinated fruit) than the haploid. In the reciprocal crosses between the doubled haploid and some diploids, many developed seeds were obtained. These seeds germinated normally and developed into diploid seedlings. These results show that the doubled haploid will be valuable for genetic analysis and possibly for planned breeding.

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“…With regard to anther culture in Citrus and their relatives, haploid plantlets have been recovered from Poncirus trifoliata L. Raf. (Hidaka et al 1979) and C. madurensis Lour. (Chen et al 1980); one DH plantlet has been obtained from hybrid 14 of C. ichangensis £ C. reticulata (Deng et al 1992).…”

Section: Introductionmentioning

confidence: 99%

Gametic and somatic embryogenesis through in vitro anther culture of different Citrus genotypes

Cardoso

Abdelgalel

Chiancone

et al. 2015

Plant Biosystems - An International Journal Dealing with all As

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In vitro tissue culture represents a useful technique for advancing Citrus breeding and propagation. Among in vitro regeneration systems, anther culture is commonly used to produce haploids and doubled haploids for a fast-track producing homozygous lines, in comparison with the traditional self-pollination approach, which involves several generations of selfing. In addition, anthers culture can produce somatic embryos that can also be used for clonal propagation. In this study, two thermal shocks were applied to the anthers of six Citrus genotypes (two clementine and four sweet oranges), just after they were put in culture. The response obtained was different depending on the genotype: both clementines, namely Hernandina and Corsica, produced homozygous and triploid regenerants (microspore-derived embryos), whereas all of the analyzed regenerants from sweet oranges, three cultivars of Tarocco and Moro, produced heterozygous and diploid regenerants similar to the parental genotypes (somatic embryos).

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In vitro Differentiation of Haploid Plants by Anther Culture in Poncirus trifoliata (L.) RAF.

Cited by 77 publications

References 10 publications

Citrus biotechnology: Achievements, limitations and future directions

Citrus biotechnology: Achievements, limitations and future directions

Morphological Characteristics of a Doubled Haploid Line from ‘Banpeiyu’ Pummelo [<i>Citrus maxima</i> (Burm.) Merr.] and Its Reproductive Function

Gametic and somatic embryogenesis through in vitro anther culture of different Citrus genotypes

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