In vitro differentiation of human bone marrow stromal cells into neural precursor cells using small molecules

Sallam, A.N.; Sudha, Thangirala; Darwish, Noureldien H. E.; Eghotny, Samar; E-Dief, Abeer; Hassaan, Passainte S.; Mousa, Shaker A.

doi:10.1016/j.jneumeth.2021.109340

Cited by 5 publications

(3 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Additionally, it has been shown that T3 and CUR could promote NSCs differentiation more efficiently than BME. Preconditioning promoted neurogenesis, proliferation and differentiation into NPCs of hMSCs, probably through the induction of key transcription factors involved in brain development and functioning, including PAX6, SOX2, DLX2 and GAP-43 [73].…”

Section: The Effects Ofmentioning

confidence: 99%

Mesenchymal Stromal Cells Preconditioning: A New Strategy to Improve Neuroprotective Properties

Schepici

Mazzon

2022

IJMS

View full text Add to dashboard Cite

Neurological diseases represent one of the main causes of disability in human life. Consequently, investigating new strategies capable of improving the quality of life in neurological patients is necessary. For decades, researchers have been working to improve the efficacy and safety of mesenchymal stromal cells (MSCs) therapy based on MSCs’ regenerative and immunomodulatory properties and multilinear differentiation potential. Therefore, strategies such as MSCs preconditioning are useful to improve their application to restore damaged neuronal circuits following neurological insults. This review is focused on preconditioning MSCs therapy as a potential application to major neurological diseases. The aim of our work is to summarize both the in vitro and in vivo studies that demonstrate the efficacy of MSC preconditioning on neuronal regeneration and cell survival as a possible application to neurological damage.

show abstract

Section: The Effects Ofmentioning

confidence: 99%

Mesenchymal Stromal Cells Preconditioning: A New Strategy to Improve Neuroprotective Properties

Schepici

Mazzon

2022

IJMS

View full text Add to dashboard Cite

show abstract

“…Identifying an optimal combination of small molecules, their concentrations and treatment durations for specific cell types requires extensive experiment. Additionally, the efficiency of chemical transdifferentiation varies across different cell types, different cell sources, et al Although a combination of small molecules successfully converted certain cells, it might not work on the others [62,63]. As Trokovic et al suggested, reprogramming efficiency correlated negatively and declined rapidly with increasing donor age [62].…”

Section: Challenges: the Bottleneck Of Chemical Transdifferentiationmentioning

confidence: 99%

Chemical Transdifferentiation of Somatic Cells: Unleashing the Power of Small Molecules

Zhang,

Li,

Xing

et al. 2023

Biomedicines

View full text Add to dashboard Cite

Chemical transdifferentiation is a technique that utilizes small molecules to directly convert one cell type into another without passing through an intermediate stem cell state. This technique offers several advantages over other methods of cell reprogramming, such as simplicity, standardization, versatility, no ethical and safety concern and patient-specific therapies. Chemical transdifferentiation has been successfully applied to various cell types across different tissues and organs, and its potential applications are rapidly expanding as scientists continue to explore new combinations of small molecules and refine the mechanisms driving cell fate conversion. These applications have opened up new possibilities for regenerative medicine, disease modeling, drug discovery and tissue engineering. However, there are still challenges and limitations that need to be overcome before chemical transdifferentiation can be translated into clinical practice. These include low efficiency and reproducibility, incomplete understanding of the molecular mechanisms, long-term stability and functionality of the transdifferentiated cells, cell-type specificity and scalability. In this review, we compared the commonly used methods for cell transdifferentiation in recent years and discussed the current progress and future perspective of the chemical transdifferentiation of somatic cells and its potential impact on biomedicine. We believe that with ongoing research and technological advancements, the future holds tremendous promise for harnessing the power of small molecules to shape the cellular landscape and revolutionize the field of biomedicine.

show abstract

“…Zhao et al demonstrated that a 115 V/m (DC) electric field for 2 h/day can promote directional migration and differentiation of neural precursor cells (NPCs) after culturing for 3 days [64]. It is important to note that hMSCs can form neuron-like cells by activating elements of the neurogenic differentiation pathway [65][66][67][68]. However, Ma et al reported that fully functional neurons cannot be produced by differentiation of MSCs, as they are ectoderm derived [69].…”

Section: Neural Differentiation Of Hmscsmentioning

confidence: 99%

Electrical Stimulation of Human Mesenchymal Stem Cells on Conductive Chitosan-Polyaniline Substrates Promotes Neural Priming

Eftekhari

Song

Janmey

2022

Preprint

View full text Add to dashboard Cite

Electrical stimulation (ES) within conductive polymer substrates has been suggested to promote the differentiation of stem cells toward a neuronal phenotype. The use of conductive scaffolds in tissue regeneration provides a unique and attractive new option to control the amount and location of ES delivery. Scaffold stiffness has also been shown to be an important regulator of stem cells' behavior and fate. Therefore, to improve stem cell-based regenerative therapies, it is essential to characterize the simultaneous effects of electroconductive substrate stiffness and electric ﬁeld stimuli on stem cell fate processes. In this study, biodegradable electroconductive substrates based on chitosan-polyaniline (CS-g-PANI) were fabricated with different stiffnesses. Human mesenchymal stem cells (hMSCs) seeded on these scaffolds were electrically stimulated for 14 days with 100 mV/ cm (20 min every day). For hMSCs cultured on soft conductive scaffolds, a morphological change with significant filopodial elongation was observed after 2 weeks of electrically stimulated culture. Compared with stiff conductive CS-g-PANI scaffolds and non-conductive CS scaffolds, for soft conductive CS-g-PANI scaffolds microtubule-associated protein 2 (MAP2) and neurofilament (NF-H) expression increased after application of ES. At the same time, there was a decrease in the expression of the glial markers glial fibrillary acidic protein (GFAP) and vimentin after ES. Furthermore, the elevation of intracellular calcium [Ca2+] during spontaneous, cell-generated Ca2+ transients further suggested that electric field stimulation of hMSCs cultured on conductive CS-g-PANI substrates can promote a neural-like phenotype. Our findings propose that the combination of the soft conductive CS-g-PANI substrate and ES is a promising new tool for enhancing nerve tissue engineering outcomes.

show abstract

In vitro differentiation of human bone marrow stromal cells into neural precursor cells using small molecules

Cited by 5 publications

References 59 publications

Mesenchymal Stromal Cells Preconditioning: A New Strategy to Improve Neuroprotective Properties

Mesenchymal Stromal Cells Preconditioning: A New Strategy to Improve Neuroprotective Properties

Chemical Transdifferentiation of Somatic Cells: Unleashing the Power of Small Molecules

Electrical Stimulation of Human Mesenchymal Stem Cells on Conductive Chitosan-Polyaniline Substrates Promotes Neural Priming

Contact Info

Product

Resources

About