"In vitro" Dissolution of Coral in Peritoneal or Fibroblast Cell Cultures

Fricain, Jean-Christophe; Bareille, Reine; Rouais, F.; Basse-Cathalinat, B.; Dupuy, B.

doi:10.1177/00220345980770020901

Cited by 15 publications

(5 citation statements)

References 29 publications

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“…Direct contact between these cells and the coral matrix is a prerequisite for the process. 22 Their finding is in accordance with the results of studies in which coral has been implanted in soft tissue sites in animals. Coral also resorbs in the absence of bone tissue, albeit at a slower rate.…”

Section: Discussionsupporting

confidence: 87%

Natural coral as bone-defect-filling material

Vuola

Böhling²,

Kinnunen

et al. 2000

J. Biomed. Mater. Res.

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Natural coral (NC) has been studied experimentally and clinically as a bone substitute, but its resorption rate and possible replacement by bone still need to be defined in humans. In this study bicortical bone was harvested from the iliac crest of 10 patients. The defect was filled with a NC block, and changes were monitored by X-rays and quantitative CT scans for a mean of 2.1 years. A biopsy was taken at 1 year. The purpose of the study was to investigate the resorption rate and pattern of NC (Porites) implants and the replacement, if any, of the implant by new bone. The blocks underwent centripetal resorption, but all the blocks still could be detected by X-rays and CT scans at the end of the follow-up period. The density of the remaining block did not change. Seven of the 10 implants were smaller than 50% of their original size at the end of the study. Bone ingrowth could be observed only in two of seven biopsies. One implant had to be removed after 1.7 years due to infection. The study shows that resorption of natural coral proceeds centripetally and apparently more rapidly when accompanied by tissue ingrowth. None of the blocks resorbed completely, and the defect at the iliac crest had not been restored by the end of the study.

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Section: Discussionsupporting

confidence: 87%

Natural coral as bone-defect-filling material

Vuola

Böhling²,

Kinnunen

et al. 2000

J. Biomed. Mater. Res.

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“…38 Moreover, fibroblasts and macrophages are involved in the resorption process dissolving coral. 39 TGF-␤ stimulates fibroblast growth 40,41 but deactivates macrophages 42 The number of macrophages in our TGF-␤1-treated implants was lower than in our coral controls [ Fig. 2(D)].…”

Section: Discussionmentioning

confidence: 77%

Transforming growth factor β released from natural coral implant enhances bone growth at calvarium of mature rat

Vuola

Böhling

Göransson

et al. 2001

J. Biomed. Mater. Res.

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Earlier studies have shown that transforming growth factor beta (TGF-beta) has the capability of enhancing bone formation after a single application to an orthotopic site. We investigated whether 1, 5, or 25 microg of recombinant human TGF-beta1 added to porous natural coral (NC) blocks could promote bone ingrowth in a critical size defect (CSD) model in nongrowing rats. A 6-mm CSD in the parietal bone of Wistar rats was filled with NC disks, which were retrieved at 3 and 8 weeks. We prepared undecalcified sections for microscopy and histomorphometry to study bone formation in the implants. The differences in the means of the measured variables were compared with a one-way analysis of variance and Tukey's Student range test, and p values smaller than 0.05 were considered statistically significant. Bone formation was enhanced in all the TGF-beta1-treated implants at 8 weeks in comparison with the controls, but none of the implants showed complete bridging across the defect. The number of macrophages and giant cells was reduced in the TGF-beta1 implants, which showed less resorption and more intact structure than the coral controls. Void areas without any fibrous tissue ingrowth were found only in the TGF-beta1-treated implants, which may partly explain the reduced resorption. The data suggested that TGF-beta1 induced enhanced but limited bone formation in mature rats and prevented resorption of the coral calcium carbonate matrix, possibly by hindering reactive cell formation and fibrous tissue ingrowth.

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“…Such an explanation is supported by literature reports20, 27 that fibroblast, macrophage, and multinucleated cell colonization precedes the appearance of osteoblasts and osteoclasts at ceramic implants in bone 27. In fact, more macrophages (cells known for phagocytosizing particles liberated by the ceramic dissolution process28) were reported at the calvaria versus the femoral sites, during the early (three week) stages of bone healing in rabbits 29. Thus, the enhanced resorption of CP observed in the presence of APL (Figures 2 and 3 of the present study) during the early stages of bone healing may be explained by an increased phagocytosis activity.…”

Section: Discussionmentioning

confidence: 69%

Effects of autologous platelet lysates on ceramic particle resorption and new bone formation in critical size defects: The role of anatomical sites

Soffer

Ouhayoun²,

Meunier

et al. 2006

J Biomed Mater Res

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This study investigated the effects of rabbit autologous platelet lysates (APL) on the performance of fillers consisting of calcium carbonate ceramic particles (CP) pertinent to new bone formation and repair. Critical-size defects in rabbit femurs and calvaria were filled with CP alone, CP plus APL, and CP plus APL with or without thrombin (THR). After 6 weeks, resorption of CP occurred under all conditions tested in the present study. Compared with respective CP alone controls, addition of APL resulted in significantly higher ceramic resorption, as evidenced by decreased ceramic particle diameter (p < 0.01) and number (p < 0.01) at both defect sites. The presence of THR prevented reduction of both CP diameter and number in the femoral defect sites. Addition of APL to the CP resulted in a significant (p < 0.03) decrease in new bone area at the calvarial sites, but not at the femoral sites; moreover, when THR was added to the CP plus APL fillers, bone formation in the femoral defects was significantly (p < 0.05) reduced. In addition to differences in the respective anatomical and cellular milieu, the biochemical events induced by mechanical loading at the femurs may explain the reduced ceramic particle resorption as well as the enhanced new bone formation when compared with the results obtained at the calvarial defect sites.

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"In vitro" Dissolution of Coral in Peritoneal or Fibroblast Cell Cultures

Cited by 15 publications

References 29 publications

Natural coral as bone-defect-filling material

Natural coral as bone-defect-filling material

Transforming growth factor β released from natural coral implant enhances bone growth at calvarium of mature rat

Effects of autologous platelet lysates on ceramic particle resorption and new bone formation in critical size defects: The role of anatomical sites

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