In vitro DNA binding studies of Aspartame, an artificial sweetener

Kashanian, Soheila; Khodaei, Mohammad Mehdi; Kheirdoosh, Fahimeh

doi:10.1016/j.jphotobiol.2013.01.001

Cited by 51 publications

(29 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…10 Sodium saccharin was previously reported to induce point mutation in the Kirsten rat sarcoma viral oncogene homolog (Kras) in human RSA cells in vitro. 12 Recently, DNA binding properties of aspartame 13 and saccharin 14 were also reported, supporting the notion that both may be genotoxic. 12 Recently, DNA binding properties of aspartame 13 and saccharin 14 were also reported, supporting the notion that both may be genotoxic.…”

Section: Introductionmentioning

confidence: 71%

Impact of aspartame and saccharin on the rat liver: Biochemical, molecular, and histological approach

Alkafafy

Ibrahim

Ahmed

et al. 2015

Int J Immunopathol Pharmacol

View full text Add to dashboard Cite

The current work was undertaken to settle the debate about the toxicity of artificial sweeteners (AS), particularly aspartame and saccharin. Twenty-five, 7-week-old male Wistar albino rats with an average body weight of 101 ± 4.8 g were divided into a control group and four experimental groups (n = 5 rats). The first and second experimental groups received daily doses equivalent to the acceptable daily intake (ADI) of aspartame (250 mg/Kg BW) and four-fold ADI of aspartame (1000 mg/Kg BW). The third and fourth experimental groups received daily doses equivalent to ADI of saccharin (25 mg/Kg BW) and four-fold ADI of saccharin (100 mg/Kg BW). The experimental groups received the corresponding sweetener dissolved in water by oral route for 8 weeks. The activities of enzymes relevant to liver functions and antioxidants were measured in the blood plasma. Histological studies were used for the evaluation of the changes in the hepatic tissues. The gene expression levels of the key oncogene (h-Ras) and the tumor suppressor gene (P27) were also evaluated. In addition to a significant reduction in the body weight, the AS-treated groups displayed elevated enzymes activities, lowered antioxidants values, and histological changes reflecting the hepatotoxic effect of aspartame and saccharin. Moreover, the overexpression of the key oncogene (h-Ras) and the downregulation of the tumor suppressor gene (P27) in all treated rat groups may indicate a potential risk of liver carcinogenesis, particularly on long-term exposure.

show abstract

Section: Introductionmentioning

confidence: 71%

Impact of aspartame and saccharin on the rat liver: Biochemical, molecular, and histological approach

Alkafafy

Ibrahim

Ahmed

et al. 2015

Int J Immunopathol Pharmacol

View full text Add to dashboard Cite

show abstract

“…Furthermore, the values of DH°and DS°were obtained to be À32.46 ± 0.20 kJ mol À1 and À29.68 ± 0.30 J mol À1 K À1 , respectively. According to the theory of Ross and Subramanian (Ross & Subramanian, 1981), negative DH°a nd DS°values were frequently regarded as evidence that the formation of kaempferol-a-glucosidase complex was an exothermic and enthalpy reaction, and both van der Waals forces and hydrogen bonds were the predominant driving forces in the kaempferol-a-glu cosidase binding reaction (Kashanian, Khodaei, & Kheirdoosh, 2013).…”

Section: Thermodynamic Parameters and Nature Of The Binding Forcesmentioning

confidence: 99%

Inhibitory kinetics and mechanism of kaempferol on α-glucosidase

et al. 2016

View full text Add to dashboard Cite

“…41 According to the association constant (K a ) of the RES-ctDNA complex at four different temperatures, the thermodynamic parameters DH and DS of the formation of RES-ctDNA were determined by van't Hoff equation: Essentially, interacting forces, including hydrogen bonding, van der Waals forces, electrostatic forces and hydrophobic interactions, play major roles in the interaction between DNA and ligands.…”

Section: Thermodynamic Parametersmentioning

confidence: 99%

Characterization of the interaction between resmethrin and calf thymus DNA in vitro

et al. 2015

View full text Add to dashboard Cite

Resmethrin (RES) is a synthetic pyrethroid insecticide widely used to control pests in agriculture, but it may cause potential hazards to human health. The characteristics of the binding of RES with calf thymus DNA (ctDNA) were investigated in a physiological buffer (pH 7.4) by multiple spectroscopic methods combined with ctDNA melting and viscosity measurements, multivariate curve resolution-alternating least-squares (MCR-ALS) chemometrics and the molecular docking technique. The concentration profiles and the pure spectra for the reactive species (RES, ctDNA and RES-ctDNA complex), obtained through decomposing the augmented UV-vis spectral data matrix by the MCR-ALS approach, indicated that RES could bind to ctDNA and the reaction process could be quantitatively monitored. The RES molecules bound to ctDNA by groove binding, as evidenced by the negligible changes in the ctDNA melting temperature, viscosity and iodide quenching effect and the increase in the single-stranded DNA quenching effect. Fluorescence titration data indicated that the complexation of RES with ctDNA was mainly driven by hydrogen bonds and van der Waals forces, and had strong affinity. The changes in the Fourier transform infrared spectra of ctDNA suggested that RES molecules preferentially bound to the G-C region of ctDNA, which was consistent with the prediction of the molecular docking. The circular dichroism spectral analysis indicated that RES induced a decrease in the right-handed helicity of ctDNA.The DNA cleavage assay showed that RES did not cleave the pUC18 plasmid DNA. This study offers a comprehensive picture of RES-ctDNA interaction, which may provide insights into the toxicological effect of the insecticide.

show abstract

In vitro DNA binding studies of Aspartame, an artificial sweetener

Cited by 51 publications

References 46 publications

Impact of aspartame and saccharin on the rat liver: Biochemical, molecular, and histological approach

Impact of aspartame and saccharin on the rat liver: Biochemical, molecular, and histological approach

Inhibitory kinetics and mechanism of kaempferol on α-glucosidase

Characterization of the interaction between resmethrin and calf thymus DNA in vitro

Contact Info

Product

Resources

About