In vitro effect of caveolin‐1 as a slow‐release material on bone‐tendon junction healing: A comparative study

Abstract

Bone tendon junction injury is hard to cure because of its special anatomical structure, and the treatment applied for bone‐tendon junction injury cannot result in the perfect vascular regeneration and restoration of the fibrocartilage zone. In this article, we aim to explore the effect of caveolin‐1 as a slow‐release material on bone‐tendon junction healing. Seventy‐two New Zealand rabbits were randomly selected and assigned into the experimental, sham‐operated and control groups (n = 24). Caveolin‐1 microspheres and microcapsule were developed as drug delivery system. At the 4th, 8th, and 12th weeks after surgery, quadriceps muscle patella‐patellar tendon (QMPPT) was obtained from each rabbit to observe the tendon‐to‐bone tunnel healing, and X‐ray examination, histological examination and biomechanical testing were applied for evaluating new bone formation. As the X‐ray showed, caveolin‐1 increased the new bone area at each time point. At the 4th and 8th weeks after surgery, the rabbit treated with caveolin‐1 slow release material showed repair of fibrocartilage. According to the biomechanical results, the cross‐sectional area, breaking load and ultimate tensile strength were increased along with time. At the same time point, caveolin‐1 increased the ultimate tensile strength. Our study demonstrates that caveolin‐1 as a slow‐release material could accelerate bone‐tendon junction healing by promoting the formation of the transition zone.

Caveolin-1 is involved in fatty infiltration and bone-tendon healing of rotator cuff tear

“…The subsequent results of this study revealed that Caveolin-1 knockdown could promote bone-tendon healing, improve biomechanical properties, and reduce the rupture risk of injured tendon after RCT in rats. In accordance with this finding, a recent work has demonstrated that Caveolin-1 can enhance biomechanical properties and accelerate bone-tendon junction healing by promoting the formation of the transition zone (Liang et al 2019). In addition, the in vitro data suggested that Caveolin-1 knockdown suppressed the adipogenic differentiation of TSCs via activation of the cAMP/PKA pathway.…”

“…Caveolin-1 is known as an oncogenic membrane protein related to endocytosis, extracellular matrix organization, cholesterol distribution, cell migration and signaling (Nwosu et al 2016). Caveolin-1 can function as a slow-release material to accelerate bone-tendon junction healing via promoting the formation of the transition zone (Liang et al 2019).…”