In Vitro Effect of Cypermethrin on the Perturbations of Rat Erythrocytes: The Ameliorative Role of Carvone

Abstract

Erythrocytes are a suitable model to study the membrane oxidative injury resulted by different xenobiotic. An attempt was made to study the antioxidant property of carvone (CAR), a monoterpene presents in herbs to mitigate toxicity of cypermethrin (CYP), a type II pyrethroid, in male rat erythrocytes in vitro. Erythrocytes were prepared and exposed to CYP (25-800 μM) alone and together with CAR (40 and 320 μM). Results showed superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) activities were significantly decreaseed. Lipid peroxidation (LPO) level, reduced glutathione (GSH) content, both of glutathione stransfarase (GST), and glutathione reductase (GR) activities were significantly increased in comparison of control value. Scanning electron microscope (SEM) supported the toxic effect of CYP which presented various degrees of alteration in shape and ruptured membranes. Pre-treatment of erythrocytes with CAR induced alleviation in all the tested parameters. It can be recommended that CYP induced oxidative stress in vitro in rat erythrocytes and supplementation with CAR ameliorated these effects.