In vitro effect of dexmedetomidine on the respiratory burst of neutrophils

Chen, S L; Zhou, Wei; Hua, Fuzhou; Chen, Y; Zheng, Xiaolan; Long, Xiang; Lu, Jie

doi:10.4238/gmr.15028069

Cited by 10 publications

(5 citation statements)

References 25 publications

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“…However, the effect of dexmedetomidine on neutrophils and lymphocytes remains controversial. One study found that dexmedetomidine suppressed the phagocytic activity of human neutrophils, which was associated with a respiratory burst coupled with NO production 19 . However, no influence of dexmedetomidine on human neutrophil or lymphocyte functions during short‐term incubation was found in vitro studies 20,21 .…”

Section: Discussionmentioning

confidence: 99%

The effect of dexmedetomidine premedication on postoperative systemic inflammatory response in children undergoing hernia repair surgery: A randomized controlled trial

Wei

Zhang

et al. 2021

Pediatric Anesthesia

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Background Surgery results in systemic inflammation, which can affect the central nervous system, leading to changes in mood, emotion, and behavior. Our previous study has shown that compared to midazolam, dexmedetomidine premedication effectively decreased children's postoperative anxiety. Aim To investigate whether dexmedetomidine infusion before hernia repair alleviates postoperative systemic inflammation in children and whether postoperative anxiety may be associated with postoperative inflammation. Methods This prospective double‐blind randomized controlled trial was conducted in 120 children scheduled to undergo elective hernia repair. Before anesthesia induction, all children received an intravenous infusion consisted of dexmedetomidine (n = 40; 0.5 µg/g, group D), midazolam (n = 40; 0.08 mg/kg, group M), or normal saline (n = 40; group C). One‐way ANOVA with least significant difference multiple comparison test was used for multigroup comparisons of postoperative plasma levels of inflammatory cytokines and m‐YPAS scores. Spearman rank correlation tests were used for analyzing m‐YPAS scores with postoperative plasma levels of inflammatory cytokines. Results Plasma levels of tumor necrosis factor‐alpha (7.0 ± 1.6 vs. 8.1 ± 1.6, mean difference [95% CI]: 1.19 [0.26–2.11], p = .008) (pg/ml) and of interleukin‐6 (1.8 ± 1.2 vs. 3.3 ± 1.6, mean difference [95% CI]: 1.49 [0.74–2.25], p < .001) (pg/ml) and neutrophils‐to‐lymphocyte ratio (1.0 ± 0.5 vs. 1.5 ± 0.7, mean difference [95% CI]: 0.48 [0.17–0.78], p < .001) were significantly lower in group D than in group C. Furthermore, compared to group M, group D showed significantly lower plasma tumor necrosis factor‐alpha levels (7.0 ± 1.6 vs. 7.9 ± 1.9, mean difference [95% CI]: 0.96 [0.04–1.88], p = .04) (pg/ml) and interleukin‐6 levels (1.8 ± 1.2 vs. 2.9 ± 1.5, mean difference [95% CI]: 1.06 [0.31–1.81], p = .004) (pg/ml), and neutrophil‐to‐lymphocyte ratio (1.0 ± 0.5 vs. 1.5 ± 0.6, mean difference [95% CI]: 0.42 [0.11–0.72], p = .004). Anxiety scores at postoperative 2 and 4 h in the three groups positively correlated with plasma levels of proinflammatory cytokines. Conclusion A single preoperative intravenous dexmedetomidine dose in children undergoing same‐day surgery reduces postoperative systemic inflammation.

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Section: Discussionmentioning

confidence: 99%

The effect of dexmedetomidine premedication on postoperative systemic inflammatory response in children undergoing hernia repair surgery: A randomized controlled trial

Wei

Zhang

et al. 2021

Pediatric Anesthesia

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“…According to this, in vitro studies have shown that clinically relevant concentrations of dexmedetomidine do not affect chemotaxis, phagocytosis, or superoxide production by human neutrophils (44). However, decreased human neutrophil phagocytosis of E. Coli, associated with suppressed respiratory burst, nitric oxide (NO) production, and induced nitric oxide synthase (iNOS) activity induced by dexmedetomidine have been reported (45).…”

Section: Sedatives and Tranquilizersmentioning

confidence: 99%

Effect of anesthetic, analgesic and sedative agents on human cell phagocytosis. Review

Mosquera

Pimienta

2020

Invest Clin

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Along with preoperative stress, anesthetics per se are associated with decreased activity of the immune system. Phagocytosis is an important process where particles, such as dead cells and bacteria, are eliminated from the organism. This process is complex and involves cell chemotaxis, tissue infiltration, several coordinated cellular events and the production of reactive oxygen and nitrogen species (ROS). Therefore, the aim of this review was to report the effects of anesthetic, analgesic and sedative agents on human cell phagocytosis. This review suggests that human phagocytosis processes are affected by main anesthetic, analgesic and sedatives agents that result in decreased chemotaxis, phagocytosis and ROS production. These effects may impair the anti-bacterial function of phagocytes. Clinical anesthesiologists should select the anesthetics and the anesthetic methods with careful consideration of the clinical situation and the immune status of patients, concerning long-term mortality, morbidity, and the optimal prognosis.

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“…For another, DEX could inhibit the maturation and function of DCs by interfering with the synthesis and secretion of IL-12 and IL-23, thereby negatively regulating human immune function ( Chen G. et al, 2016 ). In addition, DEX can inhibit the protein hydrolysis and migration of phagosomes in DCs, reduce the expression and migration of class II MHC molecule I-Ab and costimulatory molecule CD86 and inhibit the proliferation of cytotoxic T lymphocytes and the secretion of IFN-γ, thereby exerting an immunosuppressive effect ( Ueshima et al, 2013 ; Chen S. L. et al, 2016 ). However, the interesting phenomena have been found in an animal experiments, in which DEX at different concentrations may have the opposite effect on the secretion of inflammatory mediators by DCs.…”

Section: Dex's Regulatory Effect On Immune Cells and Inflammationmentioning

confidence: 99%

Effects of Dexmedetomidine on Immune Cells: A Narrative Review

Chen

Sun²,

Lv³

et al. 2022

Front. Pharmacol.

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As we all know, dexmedetomidine (DEX), as a highly selective α2 adrenergic receptor agonist, exerts sedative, anti-anxiety and hypnotic effects by inhibiting the discharge of norepinephrine neurons in locus coeruleus and GABA-related hypnotic pathways. However, the role of DEX in anti-inflammatory and immune regulation has gradually attracted the attention of researchers in recent years. The α2 adrenergic receptor is one of the members of the adrenergic receptor family, which is widely present in a variety of immune cells and mediates the biological behavior of the inflammatory immune system. At present, there have been more and more studies on the effects of DEX on immune cells and inflammatory responses, but few studies have systematically explored the anti-inflammatory and immunomodulatory effects of DEX. Here, we comprehensively review the published human and animal studies related to DEX, summarize the effects of DEX on immune cells and its role in related diseases, and propose potential research direction.

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In vitro effect of dexmedetomidine on the respiratory burst of neutrophils

Cited by 10 publications

References 25 publications

The effect of dexmedetomidine premedication on postoperative systemic inflammatory response in children undergoing hernia repair surgery: A randomized controlled trial

The effect of dexmedetomidine premedication on postoperative systemic inflammatory response in children undergoing hernia repair surgery: A randomized controlled trial

Effect of anesthetic, analgesic and sedative agents on human cell phagocytosis. Review

Effects of Dexmedetomidine on Immune Cells: A Narrative Review

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