In vitro effect of karathane LC (dinocap) on human lymphocytes

Çelık, Mustafa; Ünal, Fatma; Yüzbaşıoğlu, Deniz; Ergün, Meltem; Arslan, Orhan; Kasap, Reşat

doi:10.1093/mutage/gei013

Cited by 16 publications

(8 citation statements)

References 33 publications

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“…To assess the genetic damage induced by physical and chemical agents including pesticides, various test systems have been described in bacteria, in mammalian cells in vivo and in vitro and in plants [12–14]. Arguably, the most reliable genotoxicity evaluation for human health risk is conducted in mammals by the induction of chromosomal aberrations (CAs) and micronuclei (MN).…”

Section: Introductionmentioning

confidence: 99%

Clastogenic Effects of Glyphosate in Bone Marrow Cells of Swiss Albino Mice

Prasad

Srivastava

Singh

et al. 2009

Journal of Toxicology

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Glyphosate (N-(phosphonomethyl) glycine, C3H8NO5P), a herbicide, used to control unwanted annual and perennial plants all over the world. Nevertheless, occupational and environmental exposure to pesticides can pose a threat to nontarget species including human beings. Therefore, in the present study, genotoxic effects of the herbicide glyphosate were analyzed by measuring chromosomal aberrations (CAs) and micronuclei (MN) in bone marrow cells of Swiss albino mice. A single dose of glyphosate was given intraperitoneally (i.p) to the animals at a concentration of 25 and 50 mg/kg b.wt. Animals of positive control group were injected i.p. benzo(a)pyrene (100 mg/kg b.wt., once only), whereas, animals of control (vehicle) group were injected i.p. dimethyl sulfoxide (0.2 mL). Animals from all the groups were sacrificed at sampling times of 24, 48, and 72 hours and their bone marrow was analyzed for cytogenetic and chromosomal damage. Glyphosate treatment significantly increases CAs and MN induction at both treatments and time compared with the vehicle control (P < .05). The cytotoxic effects of glyphosate were also evident, as observed by significant decrease in mitotic index (MI). The present results indicate that glyphosate is clastogenic and cytotoxic to mouse bone marrow.

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Section: Introductionmentioning

confidence: 99%

Clastogenic Effects of Glyphosate in Bone Marrow Cells of Swiss Albino Mice

Prasad

Srivastava

Singh

et al. 2009

Journal of Toxicology

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“…A decrease in mitotic index has also been reported to many other pesticides such as etoxazole, karathane LC and sodium arsenite [26][27][28]. However, this fungicide did not affect the RI value.…”

Section: Discussionmentioning

confidence: 59%

“…In addition, in human lymphocytes, the lowest dose induced significant increase in both abnormal cells (%) and CA/cells. These are chromatid break, fragment, sister chromatid union, dicentric chromosomes, gap, ring chromosomes, chromatid exchanges and polyploidy [27]. The frequency of structural chromosome aberrations and CA/cell ratio were higher in human lymphocyte than those of mouse bone marrow cells, after the treatment of Conan 5FL.…”

Section: Discussionmentioning

confidence: 90%

10.1007/s11177-008-3005-z

2010

CrossRef Listing of Deleted DOIs

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The genotoxic effects of fungicide Conan 5FL (containing 50 g/L hexaconazole) in mouse bonemarrow cells and human lymphocytes have been evaluated. Three different concentrations of Conan 5FL (17.50, 35.0, and 70.0 µ g/mL for human lymphocytes and 17.50, 35.0, and 70.0 mg/kg for mouse bone marrow cells) were studied. Conan 5FL induced significant increases (except 17.5 mg/kg for mouse bone marrow) in the frequency of chromosomal aberrations (CAs) in both test systems. This fungicide caused structural and numerical abnormalities in both mammalian cells. These are sister chromatid union, chromatid and chromosome breaks, fragments, dicentric and ring chromosomes, and polyploidy. Significant increase was found in induction and in minimum-maximum numbers of sister chromatid exchanges (SCEs) at all treatments compared with the negative control. Conan 5FL did not affect the replication index (RI) in human lymphocyte cultures, however, it significantly decreased the mitotic index (MI) in all treatment concentrations in both test systems. Using of Conan 5FL should be reconsidered due to its possible cytotoxic, clastogenic and mutagenic effects.

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“…Pesticides are very reactive compounds forming covalent bonds with various cellular biomolecules and, consequently, can damage cell structures and/or interfere with metabolic processes. Several studies have been conducted on different animal models and cellular systems to investigate the genotoxic effects of pesticides (Bolognesi, 2003;Çelik et al, 2004;Poletta et al, 2009). The induction of CAs formation by exposition to different pesticides has been reported in several different test systems (Bolognesi, 2003).…”

Section: Discussionmentioning

confidence: 99%

Chromosomal aberrations in cultured human lymphocytes treated with the fungicide, Thiram

Santovito¹,

Cervella²,

Delpero³

2012

Drug and Chemical Toxicology

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In vitro effects of different concentrations of Thiram were tested on human lymphocytes to determine, by means of the chromosome aberrations (CAs) assay, whether this fungicide could induce clastogenic damage. Evidences of the effect of Thiram on human lymphocytes were limited to sister chromatid exchange, micronuclei formation, and comet assays. We evaluated 0.01, 0.1, 1.2, and 12.0 μg/mL of Thiram, where 0.01 μg/mL represent the acceptable daily intake dose set by the World Health Organization and the Food and Agriculture Organization for fruit and vegetables, whereas 0.1, 1.2, and 12.0 μg/mL are its multiple values. Results indicated that human lymphocytes treated in vitro with Thiram at concentrations of 1.20 and 12.0 μg/mL significantly increased CAs frequency, compared with the negative control, whereas at lower concentrations (0.01 and 0.1 μg/mL), this effect was not observed. However, Thiram showed a clastogenic effect also at the concentration value of 1.2 μg/mL that represents a lower value with respect to the residue limits found in Italy for grapes, strawberries, potatoes, tobacco, and other fruits and vegetables. Finally, according to some evidence obtained from the study of other fungicides, Thiram produced a significant reduction in the mitotic index with increasing concentration.

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In vitro effect of karathane LC (dinocap) on human lymphocytes

Cited by 16 publications

References 33 publications

Clastogenic Effects of Glyphosate in Bone Marrow Cells of Swiss Albino Mice

Clastogenic Effects of Glyphosate in Bone Marrow Cells of Swiss Albino Mice

10.1007/s11177-008-3005-z

Chromosomal aberrations in cultured human lymphocytes treated with the fungicide, Thiram

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