In Vitro Effect of Methanolic Extract of Argemone mexicana against Trichomonas vaginalis

Elizondo-Luévano, Joel H.; Verde-Star, Julia; González-Horta, Azucena; Castro‐Ríos, Rocío; Hernández-García, Magda Elizabeth; Chávez-Montes, Abelardo

doi:10.3347/kjp.2020.58.2.135

Cited by 22 publications

(19 citation statements)

References 29 publications

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“…Berberine at 5 μM is effective against S. mansoni at 48 h, this dose is far below the lowest concentration evaluated in the cytotoxicity test, berberine stands out since in minimal doses it′s biological activity is very effective. These data are consistent with those previously reported ( 15 ). Therefore, at concentrations of interest, below 200 μg/mL ( Table 2 ), Am and berberine were not found to be significantly cytotoxic ( P <0.001).…”

Section: Discussionsupporting

confidence: 94%

“…The control of schistosomiasis is based on praziquantel which has many deficiencies ( 22 ). Many investigations have evaluated several substances from plant origin, such as berberine, abundant in A. mexicana , shown promising properties against L. tropica , L. infantum , Trichomonas vaginalis, and E. histolytica ( 15 , 23 , 24 ). However, no studies have been conducted against S. mansoni .…”

Section: Discussionmentioning

confidence: 99%

“…The solvent was removed into a rotary evaporator and the extract was concentrated to dryness and stored at −20 °C, until testing. The functional groups present in the methanolic extract and the active component berberine had been previously determined in a publication previously published by our group in 2020 ( 15 ).…”

Section: Methodsmentioning

confidence: 99%

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In Vitro Antischistosomal Activity of the Argemone mexicana Methanolic Extract and Its Main Component Berberine

Elizondo-Luévano¹,

Castro‐Ríos²,

Vicente³

et al. 2021

IJPA

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Background: Schistosomiasis has been identified as a major public health problem in tropical countries. The present study aimed to investigate the schistosomicidal effects of the methanolic extract of Argemone mexicana L. and its active component, berberine against Schistosoma mansoni on in-vitro experiments. Methods: S. mansoni adults were used. Various concentrations of the methanolic extract (10 - 200 µg/ml) and berberine (2.5 - 50 µM) were tested from 24 to 72 h. The viability of S. mansoni was confirmed with an invertoscope-microscope. Furthermore, cytotoxic (Hemolysis test), and antioxidant (DPPH radical scavenging assay) capacities were determined. Results: The viability tests on S. mansoni showed that A. mexicana at 50 μg/mL is lethal at 48 h and berberine at 10 μM is lethal at 24 h. The hemolytic activity at 1,000 μg/mL was 2.9% for A. mexicana and 90.2% for berberine. The antioxidant capacities shown by A. mexicana and berberine, were EC50 156.3 and 84.1 μg/mL, respectively. Conclusion: The extract of A. mexicana and berberine demonstrated high antischistosomal activities in low concentration and short exposure time on the in-vitro model.

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Section: Discussionsupporting

confidence: 94%

Section: Discussionmentioning

confidence: 99%

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In Vitro Antischistosomal Activity of the Argemone mexicana Methanolic Extract and Its Main Component Berberine

Elizondo-Luévano¹,

Castro‐Ríos²,

Vicente³

et al. 2021

IJPA

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“…Considering berberine as one of the important constituents of plant Argemone mexicana , very recently, Elizondo-Luevano et al (2020) compared the anti-trichomonal effect of methanolic extract of leaves and stem of the plant with pristine berberine and metronidazole as a standard control. The IC 50 values of stem and leaf extracts of Argemone mexicana against T. vaginalis were 70.8 and 67.2 μg/mL, respectively.…”

Section: Resultsmentioning

confidence: 99%

A review study on the anti-trichomonas activities of medicinal plants

Hashemi

Ommi

Kheyri

et al. 2021

International Journal for Parasitology: Drugs and Drug Resistan

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The parasitic diseases represent the most important health risk, especially in underdeveloped countries where they have a deep impact on public health. Trichomoniasis is a prevalent non-viral sexually transmitted disease, and a significant amount of new cases are identified each year globally. Furthermore, the infection is linked with serious concerns such as pregnancy outcomes, infertility, predisposition to cervical and prostate cancer, and increased transmission and acquisition of HIV. The therapy is restricted, adverse effects are often observed, and resistance to the drugs is emerging. Based on this, a new treatment for trichomoniasis is necessary. Natural products represent a rich source of bioactive compounds, and even today, they are used in the search for new drugs. Additionally, natural products provide a wide variety of leadership structures that can be used by the pharmaceutical industry as a template in the development of new drugs that are more effective and have fewer or no undesirable side effects compared to current treatments. This review focuses on the medicinal plants that possess anti-trichomonal activity in vitro or in vivo . An electronic database search was carried out covering the last three decades, i.e., 1990–2020. The literature search revealed that almost a dozen isolated phytoconstituents are being explored globally for their anti-trichomonal activity. Simultaneously, many countries have their own traditional or folk medicine for trichomoniasis that utilizes their native plants, as a whole, or even extracts. This review focuses mainly on the human parasite Trichomonas vaginalis . However, at some points mention is also made to Tritrichomonas foetus that causes trichomoniasis in animals of high veterinary and economical interest. We will focus on the plants and plant-based compounds and their anti-trichomonal activity. The literature search highlighted that there are abundant compounds that possess anti-trichomonal activity; however, in-depth in-vivo evaluation of compounds and their clinical evaluation has not been undertaken. There is a critical need for new anti-trichomonal compounds, and focused research on phytoconstituents can provide the way forward.

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“…Para la evaluación de la hemólisis, la suspensión se incubó con diferentes concentraciones de Ber, Cur o Qr, y de soluciones testigo previamente preparadas en MeOH absoluto (50 a 1 000 μg/ ml) en viales de 2 ml durante 30 min a 37 °C y en ausencia de luz, estos fueron catalogados como tratamientos (Tr). Como control negativo (C-) se utilizó eritrocitos sin tratamiento y como control positivo (C+) eritrocitos con agua destilada estéril para producir la hemólisis osmótica en los eritrocitos (Elizondo-Luevano et al, 2020). Para la evaluación del efecto citoprotector, a los tratamientos se les añadió AAPH (150 mM en PBS) más la suspensión de eritrocitos y se incubaron a 37 °C (5 h a 200 rpm) utilizando una incubadora de rotación MaxQ™ (Thermo-Scientific™, USA), en ausencia de luz.…”

Section: Materiales Y Métodosunclassified

Berberina, curcumina y quercetina como potenciales agentes con capacidad antiparasitaria

Elizondo-Luévano

Hernández-García

Pérez-Narváez

et al. 2020

RBT

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Introducción: El uso indiscriminado de agentes antiparasitarios ha resultado en el establecimiento de resistencia a ellos. Por lo cual es necesario el desarrollo de nuevas alternativas de tratamiento. Los productos naturales poseen diversas cualidades como posibles coadyuvantes en terapias contra distintos agentes etiológicos, entre los que destaca sus efectos antiparasitarios. Objetivo: Evaluar la actividad antiparasitaria, antioxidante, citotóxica y citoprotectora de Berberina (Ber), Curcumina (Cur) y Quercetina (Qr). Metodología: Se prepararon soluciones de Ber, Cur y Qr grado analítico y se realizaron alícuotas a diferentes concentraciones para su evaluación en contra de: Entamoeba histolytica, Trichomonas vaginalis y Strongyloides venezuelensis, para ello, se determinó la concentración inhibitoria media (IC50), además se determinó la capacidad antioxidante (CE50) mediante la prueba de DPPH, ambos por la prueba de Probit. Mediante la técnica de hemólisis se determinó la actividad citotóxica y citoprotectora, se aplicó Anova y la prueba de Tukey para determinar la diferencia de las medias en los tratamientos evaluados. Resultados: Ber, Cur y Qr, presentaron actividad en contra de E. histolytica, T. vaginalis y S. venezuelensis in-vitro. Ber presentó IC50 de 1.7, 1.2 y 1.9 μM respectivamente siendo más efectivo en comparación de Cur con IC50 de 55.3, 40.6 y 13.7 μM o Qr con IC50 de 147.2, 93.2 y 110.9 μM, sin embargo, la mejor actividad antioxidante (EC50 = 1.1 μg/ml), citoprotectora y menos hemolítica, fue presentada por Qr (P < 0.001) en comparación con el control evaluado. Conclusiones: Los metabolitos de origen natural berberina, curcumina y quercetina, poseen actividad en contra de trofozoítos de E. histolytica, T. vaginalis y larvas de S. venezuelensis en dosis bajas comparables con los fármacos de referencia para el caso de Ber. Además, estos productos de origen natural, no sintético podrían ser objeto de futuras investigaciones para coadyuvar al tratamiento de parasitosis, ya que, en dosis bajas, mostraron actividad antioxidante sin mostrar hemólisis considerable en eritrocitos humanos.

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In Vitro Effect of Methanolic Extract of Argemone mexicana against Trichomonas vaginalis

Cited by 22 publications

References 29 publications

In Vitro Antischistosomal Activity of the Argemone mexicana Methanolic Extract and Its Main Component Berberine

In Vitro Antischistosomal Activity of the Argemone mexicana Methanolic Extract and Its Main Component Berberine

A review study on the anti-trichomonas activities of medicinal plants

Berberina, curcumina y quercetina como potenciales agentes con capacidad antiparasitaria

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