In vitro effect of polyethylene glycol and sorbitol on two banana varieties viz. Grand naine and Nalla bontha to study drought stress

Abstract

Water stress is one of the foremost categories of stress damaging plants’ overall growth and development. The aim of the present study was to explore and demonstrate stress-induced drought to calibrate changes in stress parameters of two banana plant varieties viz. Grand naine (G9) and Nalla bontha were cultured in Murashige and Skoog medium (MS) media supplemented with stress inducers -Poly ethylene glycol (PEG) and sorbitol. The different concentrations of inducers were used to induce drought stress in two varieties of banana with contrasting characters for drought condition. The study indicated that PEG contrived mild to moderate osmotic stress and so does the alditol i.e. sorbitol on in vitro banana plants. The different concentrations of PEG and sorbitol produced significant effects on various parameters. The maximum decrease in shoot length was prominent in G9(52%) as compared to Nalla bontha (11%). When treated with 3% w/v sorbitol, even average root length showed the same level of damage with G9(59%) while in case of Nalla bontha there was a 17% decrease. The banana plantlet produced in vitro was estimated at one and two weeks after inoculation, respectively. Proline content tended to increase as the concentration of osmotic inducers increased (-44 % in G9 at 3% w/v sorbitol), whereas RWC (8.9% in G9 at 3% w/v Peg) showed an opposite effect. It was concluded that quantitative and qualitative changes in physiological (shoot and root length) and biochemical (Proline and relative water content, RWC) parameters played an important role in plants under drought stress conditions. This pattern varied from species to species. This work has been attempted for the first time in banana, especially Grand naine varieties with contrasting characters under induced drought stress.

Improvement of Selected Morphological, Physiological, and Biochemical Parameters of Banana (Musa acuminata L.) Using Potassium Silicate under Drought Stress Condition Grown in vitro

“…Al-Mayahi [51] found that adding PEG at 15% to MS media caused an increase in proline content of P. dactylifera L compared with control Re-culturing the micro-shoots of P. dactylifera L on MS media supplemented with Si at 3.6 mM combined with 15% PEG led to a decrease in proline content compared with those of control and culture media supplemented with PEG only. In addition, Singh et al [62] illustrated that adding PEG at 1% to 3% to the growth MS media caused an increase in proline content of two banana varieties viz. Grand naine and Nalla bontha compared with those of control and other treatments [62].…”

“…In addition, Singh et al [62] illustrated that adding PEG at 1% to 3% to the growth MS media caused an increase in proline content of two banana varieties viz. Grand naine and Nalla bontha compared with those of control and other treatments [62]. In Arabidopsis, higher levels of proline accumulation were linked to improved salt and drought resistance [63].…”

Germinación asimbiótica, conservación ex situ e in vitro regeneración de plantas de Catasetum integerrimum Hook

“…Orchids are also considered an important crop in the floriculture industry. This plant family is usually cultured due to its medicinal properties and uses in traditional food, as well as the generation of nutraceutical products, perfumes, and cosmetics (Hinsley et al, 2018;Kanlayavattanakul et al, 2018;Salazar-Mercado & Botello-Delgado, 2020;Singh et al, 2021). In recent years, many species of orchids have been threatened in their natural habitats because of the environmental pressure generated by humans (Juras et al, 2020;Fonseka, 2020;Puspitaningtyas & Handini, 2020).…”