In vitro effect of zearalenone on sperm parameters, oocyte maturation and embryonic development in buffalo

Yousef, Mohamed Samy; Rezk, Walaa R.; El-Naby, Al-Shimaa Al-H H; Mahmoud, Karima Gh. M.; Takagi, Mitsuhiro; Miyamoto, Akio; Megahed, Gaber

doi:10.1016/j.repbio.2023.100732

Cited by 6 publications

(3 citation statements)

References 46 publications

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“…In contrast, in vitro exposure of bovine spermatozoa to ZEN or T2 (0.5 mM to 0.01 mM) for 1 h induced severe damage to the membrane [ 52 ]. Recently, it was reported that in vitro exposure of buffalo spermatozoa to ZEN at 2000 ng/mL for 2 h impaired their viability, whereas lower concentrations (i.e., 10, 100, and 1000 nb/mL did not [ 53 ]. Similarly, in vitro exposure of boar semen to 62.8 µM ZEN for 1, 2, 3, and 4 h reduced the spermatozoa viability [ 54 ].…”

Section: Discussionmentioning

confidence: 99%

The Effect of Mycotoxins and Their Mixtures on Bovine Spermatozoa Characteristics

Kalo,

Mendelson,

Komsky-Elbaz

et al. 2023

Toxins

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There is growing concern about the effects of mycotoxins on mammalian reproduction. Although the effects of single mycotoxins have been well documented, the impact of their mixtures on spermatozoon quality is less known. Here, frozen-thawed semen (n = 6 bulls) was in-vitro-cultured (2 h) without (control) or with (i) a single mycotoxin [zearalenone (ZEN), ochratoxin A (OTA), toxin 2 (T2), and diacetoxyscirpenol (DAS)] in a dose-response manner; (ii) binary mixtures (OTA + T2, OTA + ZEN, OTA + DAS, ZEN + T2, DAS + T2 and ZEN + DAS); or (iii) ternary mixtures (OTA + DAS + T2, OTA + ZEN + T2, and ZEN + DAS + T2). Then, the spermatozoa quality was characterized according to its plasma- and acrosome-membrane integrity, mitochondrial membrane potential, and oxidation status by a flow cytometer. Exposure to single mycotoxins or binary mixtures did not affect the spermatozoa characteristics. However, exposure to the ternary mixtures, OTA + DAS + T2 and OTA + ZEN + T2, reduced (p < 0.05) the mitochondrial membrane potential relative to the control. In addition, OTA + ZEN + T2 increased (p < 0.05) the proportion of spermatozoa with reactive oxygen species relative to the control. The most suggested interaction effect between the mycotoxins was found to be an additive one. A synergistic interaction, mainly regarding the oxidation status of the spermatozoa, was also found between the mycotoxins. The current study sheds light on the potential risk of exposing spermatozoa to a mycotoxin mixture.

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Section: Discussionmentioning

confidence: 99%

The Effect of Mycotoxins and Their Mixtures on Bovine Spermatozoa Characteristics

Kalo,

Mendelson,

Komsky-Elbaz

et al. 2023

Toxins

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“…GH receptors are found in the granulosa and theca cells of the ovaries, oocytes, cumulus cells, mammary glands, placenta, and uterus. Attachment of GH to its receptors causes modulation of steroid hormone and gamete development, enhancement of the development, reproduction, and maturation of gametes and follicles, regulation of gonadotropin secretion, and improvement of embryo quality and endometrial reception ( 4 – 7 ).…”

Section: Introductionmentioning

confidence: 99%

The Impact of Growth Hormone Co-Treatment Duration on Outcomes in IVF/ ICSI Cycles Among Poor Ovarian Responders

Mohammadshirazi,

Alyasin,

Hosseini

et al. 2023

JRI

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Background: The efficiency of in vitro fertilization is improved by growth hormone (GH) during ovarian stimulation. Additionally, patients with diabetes experience im-paired insulin resistance and compromised glucose tolerance, which further ex-acerbate their condition. Due to these side effects, in this study, the duration of GH treatment was compared in IVF/ICSI cycles among poor ovarian responders. Methods: In this study, POSEIDON criteria were used to choose patients. Sub-cutaneous administration of gonadotropin-releasing hormone (GnRH) antagonist was done beginning on the sixth day of the cycle and continuing through the day of human chorionic gonadotropin (hCG) injection. In one group, GH was administered 4 units/day from the 2 nd day of the cycle until hCG injection, and in another group, the first dose was administered on the 6th day of the cycle. Following the admin-istration of hCG, which lasted from 24 to 36 hr, oocytes were retrieved with the support of B-mode sonography. Results: In our analysis, no significant differences were observed between the two groups in terms of the number of retrieved oocytes, metaphase II oocytes, and quality of grade A and B embryos. The results show that the treatment or conditions did not have a significant impact on the outcomes among the studied groups. Conclusion: Our findings indicate that a shorter duration of GH administration can yield similar outcomes compared to a longer duration in IVF/ICSI cycles involving poor ovarian responders. This result holds the potential for a more cost-effective and patient-friendly approach in managing assisted reproductive technology procedures. It may lead to reduced side effects and improved adherence to medication regimens in patients.

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“…Not only DON and ZEN but also DOM-1, the de-epoxy metabolite on DON, have been detected in ovarian follicular uid of cows, suggesting that following ingestion, signi cant amounts of these mycotoxins can be transported to the ovary and may locally interfere with reproductive function in cattle (Takagi et al, 2008;Winkler et al, 2015). Several in vitro studies have reported a number of adverse effects in ZENtreated bovine oocytes, including alteration of nuclear maturation and developmental ability after fertilization (Minervini et al, 2001;Takagi et al, 2008;Yousef et al, 2023). Likewise, in vitro exposure to DON has been shown to increase apoptosis of bovine theca and granulosa cells and to decrease progesterone and estradiol production (Guerrero-Netro et al, 2015;Guerrero-Netro et al, 2017;Pizzo et al, 2016;Pizzo et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Real-life exposure to Fusarium toxins deoxynivalenol and zearalenone triggers apoptosis and activates NLRP3 inflammasome in bovine primary theca cells

et al. 2023

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Cattle are deemed less susceptible to mycotoxins due to the limited internal exposure resulting from rumen microbiota activity. However, the signi cant amounts of Fusarium mycotoxins deoxynivalenol (DON) and zearalenone (ZEN) frequently detected in bovine follicular uid samples suggest that they could affect ovarian function.Both mycotoxins trigger several patterns of cell death and activate the NLRP3 in ammasome in the intestine. In vitro studies have reported a number of adverse effects on bovine oocytes. However, the biological relevance of such ndings with regard to realistic concentrations of DON and ZEN in bovine follicular uid is still not clear. Hence, it is important to better characterize the effects of dietary exposure to DON and ZEN on the bovine ovary.Using bovine primary theca cells, this study investigated the effects of real-life patterns for bovine ovary exposure to DON and ZEN, but also DON metabolite DOM-1, on cell death and NLRP3 in ammasome activation.Exposure to DON starting from 0.1µM signi cantly decreased theca cell viability. The kinetics of phosphatidylserine translocation and loss of membrane integrity showed that ZEN and DON, but not DOM-1, induce an apoptotic phenotype. qPCR analysis of the expression of NLRP3, PYCARD, IL-1β, IL-18, and GSDMD in primary theca cells at concentrations of mycotoxin previously reported in cow follicular uid clearly indicated that DON and DOM-1 individually and in mixture, but not ZEN, activate NLRP3 in ammasome.Altogether, these results suggest that current European Union guidance values for DON may not protect the bovine ovary from in ammatory disorders.

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In vitro effect of zearalenone on sperm parameters, oocyte maturation and embryonic development in buffalo

Cited by 6 publications

References 46 publications

The Effect of Mycotoxins and Their Mixtures on Bovine Spermatozoa Characteristics

The Effect of Mycotoxins and Their Mixtures on Bovine Spermatozoa Characteristics

The Impact of Growth Hormone Co-Treatment Duration on Outcomes in IVF/ ICSI Cycles Among Poor Ovarian Responders

Real-life exposure to Fusarium toxins deoxynivalenol and zearalenone triggers apoptosis and activates NLRP3 inflammasome in bovine primary theca cells

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