In vitro effects of a natural marine algae polysaccharide (λ-carrageenan) on seabream erythrocytes, tumour cell lines and marine bacterial pathogens

Abstract

Carrageenan is a high molecular weight sulphated polysaccharide used to induce experimental inflammation in mammals. In addition, it possesses a wide variety of properties that have not yet been studied in fish. This study evaluated the hemagglutinating, hemolytic, cytotoxic, and antibacterial activities of λ-carrageenan. The results showed that λ-carrageenan has hemagglutinating and hemolytic activities on gilthead seabream erythrocytes, which were dose and time-dependent during the first 6 h of incubation. No significant effects on the haemolytic activity of erythrocytes were observed after incubation for 12 or 24 h with λ-carrageenan. The PLHC-1 cell line showed significant increases in cytotoxic activity after 6 or 12 h of incubation compared with control cells, and the highest doses of λ-carrageenan caused cytotoxicity in PLHC-1 cells after 24 h of incubation. The morphology of PLHC-1 cells incubated with the highest doses of λ-carrageenan for 12 or 24 h showed obvious cell death changes compared with control cells. Interestingly, no significant variations in cytotoxic activity were observed in SAF-1 cell line after incubation with λ-carrageenan. Furthermore, λ-carrageenan showed significant dose-dependent bactericidal activity against Photobacterium damselae but had no significant effect on the bactericidal activity of Vibrio harveyi, Vibrio anguillarum, and Tenacibaculum maritimum. The study suggests that λ-carrageenan has potential applications in aquaculture and aquatic pharmaceutical industries as a hemagglutinating, hemolytic, and antibacterial agent.

Carboxymethyl kappa carrageenan derivatives synthesized from kappa carrageenan of the red alga Kappaphycus striatus cultivated in Vietnam: Characterization, structure and biological activity

“…It is also suggested that the positive charges of glycoprotein on surface of the erythrocyte membrane could be attracted by the negative charges of kappa carrageenan or their carboxymethylated derivatives, result increases the ability of erythrocytes to adhere and form agglutination (Wang et al 2021). The similar agglutination has also been recorded for carrageenan (Silva et al 2010;McKim 2014;Campos-Sánchez et al 2023). of the red alga K. striatus showed the physicochemical properties, structure and biological activities similar well to those of carboxymethyl kappa carrageenans reported, including degree and sites of carboxymethyl group substitution in kappa carrageenan structure, molecular weights, antioxidant and hemagglutination activity.…”