In-vitro effects of almond oil, barks and leaves extracts of Baillonella toxisperma (Pierre) against Trichophyton soudanense and Trichophyton rubrum

Abstract

The purpose of this study was to evaluate anti-trichophytic effects of Baillonella toxisperma (Pierre) extracts against Trichophyton rubrum and Trichophyton soudanense. The tests were performed by the agar dilution method and the results have showed the effective inhibition of the mycelia growth of theses strains by B. toxisperma (Pierre) extracts. After 7 days of incubation at 28°C, the percentages of mycelia growth inhibition PI (%) by plant extracts and fluconazole were range between 20.81 and 100% on T. soudanense, and between 19.92 and 100% on T. rubrum. Some B. toxisperma (Pierre) extracts were exhibited similar anti-trichophyton activities compared to those obtained with fluconazole which was used like reference antifungal drug (p < 0.05). The best anti-trichophyton activities of B. toxisperma (Pierre) extracts were obtained at 5 mg/ml on T. Rubrum with almond oil and hexane phase of water-hexane partition of hydro-ethanol crude extract of barks bromobentseeni (HBb), and at 10 mg/ml on T. soudanense with (HBb). This anti-trichophyton activity of B. toxisperma (Pierre) extracts was attributed to secondary metabolites. The phytochemical analysis has showed that the plant contain bioactive metabolites groups such as phenols, flavonoids, saponins, coumarin, tanins, anthocyanins, lipids and triterpenes. The metabolic groups have been mentioned in several studies for their antifungal activities. The results from this study provide a scientific validation for the ethnomedicinal uses of B. toxisperma (Pierre) in the treatment of fungal infections.