In vitro effects of aqueous extract of fermented rooibos (Aspalathus linearis) on human sperm function

Takalani, Ndivhuho Beauty; Adefolaju, Gbenga Anthony; Henkel, Ralf; Opuwari, Chinyerum Sylvia

doi:10.1111/and.14114

Cited by 7 publications

(5 citation statements)

References 59 publications

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“…The washed samples were incubated with black tea aqueous extract (0.4, 4.0, 40, and 405 μg/ml) for 1 hr at 37°C. Additional sperm parameters, sperm motility, vitality, mitochondrial membrane potential (MMP), ROS production, DNA fragmentation, capacitation, and acrosome reaction, were analyzed as previously described [32,[35][36][37][38]. The control contained only HTF-BSA.…”

Section: Methodsmentioning

confidence: 99%

“…The SCA was used to assess the motility of treated sperm suspension (2 μl) on Leja slides and incubated at 37°C. Moreover, analysis was done on the following: beat cross frequency (BCF), linearity (LIN), straightness (STR), amplitude of lateral head displacement (ALH), wobble (WOB), average velocity path (VAP), velocity curve line (VCL), velocity straight line (VSL), and total motility [32,37].…”

Section: Sperm Motility and Kinematicmentioning

confidence: 99%

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Black Tea Aqueous Extracts Improve Human Sperm Functions: An In Vitro Study

Setumo,

Choma,

Henkel

et al. 2023

Andrologia

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Infertility affects about 25% of couples worldwide, and oxidative stress (OS) is linked to its idiopathic etiology. Green, black, white, and oolong teas are produced from Camellia sinensis, depending on their oxidation level, and contain antioxidant properties that may enhance male reproductive functions. The study aimed to investigate the effects of black tea aqueous extract on human sperm functions in vitro. Semen samples were collected from donors, liquefied, analyzed, and divided into normal (n = 40) and abnormal (n = 19) groups using the World Health Organization 2010 criteria. Samples were washed and incubated with black tea aqueous extracts (0, 0.4, 4, 40, 405 μg/ml) for 1 hr and analyzed. Along with a considerable decrease in intracellular reactive oxygen species (ROS) production, DNA-fragmented spermatozoa, and acrosome reaction, the percentage of sperm vitality and intact mitochondrial membrane potential (MMP) increased (p<0.05). Furthermore, compared to the normal group, a substantial increase in the percentage of acrosome reaction, ROS production, and percentage of spermatozoa with fragmented DNA, while a reduction in the percentage of intact MMP and sperm vitality, was noted in the abnormal group (p<0.05). Compared to the controls, there was no significant change in motility between the normal and abnormal groups (p>0.05). Black tea’s antioxidant activity, caffeine concentration, or both may have contributed to its improvement in human sperm function in vitro.

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Section: Methodsmentioning

confidence: 99%

Section: Sperm Motility and Kinematicmentioning

confidence: 99%

Black Tea Aqueous Extracts Improve Human Sperm Functions: An In Vitro Study

Setumo,

Choma,

Henkel

et al. 2023

Andrologia

Self Cite

View full text Add to dashboard Cite

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“…Another study also reported on the reduction of sperm DNA aberration after treatment with a variety of vitamins E and C (400 mg each), β-carotene (18 mg), zinc selenium, or a mixture of 30 mg β-carotene, 180 mg of vitamin E and fatty acids ( Punjabi et al 2022 ). Furthermore, several studies have revealed antioxidant therapy to improve sperm quantity and quality ( Büyükleblebici et al 2014 , Moichela et al 2021 , Takalani et al 2021 , Setumo et al 2022 ).…”

Section: Management Of Mosimentioning

confidence: 99%

Role of oxidative stress in male infertility

Takalani,

Monageng,

Mohlala

et al. 2023

Reproduction and Fertility

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Infertility affects millions of couples worldwide. Research has highlighted that oxidative stress (OS) causes peroxidation of lipids as well as damage to spermatozoa which reduces the quality of seminal parameters. The differences in the levels of antioxidants and reactive oxygen species (ROS) caused by intrinsic and extrinsic variables linked to lifestyle, diet, genetics, and OS also contributes to male infertility. High levels of ROS result in sperm damage from lipid peroxidation. Other significant causes of ROS include changes in sex hormone levels, genetic mutations, and immature spermatozoa. Treating the root causes of OS, by changing one's lifestyle, as well as antioxidant therapy may be helpful strategies to fight infertility brought on by OS. However, the determination of male infertility induced by OS is currently a challenge in the field of reproductive health research. This article gives a brief overview of the progression of OS in semen samples and its pathology in male fertility as well as techniques for managing infertility caused by OS.

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“…The samples were mixed with HTF-BSA (1:6) (Moichela et al 2021;Takalani et al 2021), washed by centrifugation (300×g; 10 min) and re-suspended. Sperm suspensions (15 × 10 6 /mL) were subsequently incubated for 1 h with green tea aqueous extracts (0 μg/mL, 0.4 μg/mL, 4.0 μg/mL, 40 μg/mL and 405 μg/mL) at 37 o C. Human tubular fluid supplemented with BSA served as the control.…”

Section: Collection and Preparation Of Human Semenmentioning

confidence: 99%

Green tea (Camellia sinensis) aqueous extract improved human spermatozoa functions in vitro

Setumo

Choma

Henkel

et al. 2022

JOMPED

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Background: Idiopathic causes of infertility is associated with oxidative stress. Antioxidants are known to scavenge the excessive production of reactive oxygen species (ROS). Green tea (Camellia sinensis) contains polyphenols that enhance its antioxidant potential.Aim: This study focused on the impact of aqueous green tea extract on normozoospermic human spermatozoa.Setting: Department of Medical Biosciences, University of the Western Cape (UWC), South Africa.Methods: Semen samples obtained using masturbation method following three to five days of sexual abstinence from consenting men (n = 59) at the University of the Western Cape (UWC) were liquefied and analysed. Normozoospermic samples were selected according to the World Health Organization (WHO) 5th guideline. Thereafter, semen samples (7.5 × 106 /mL) were washed in human tubular fluid (HTF; 10 min at 300 ×g) and exposed to aqueous extracts of green tea (0 μg/mL, 0.4 μg/mL, 4 μg/mL, 40 μg/mL, 405 μg/mL) for 1 h with various sperm parameters analyzed. Human tubular fluid supplemented with bovine serum albumin (HTF-BSA; 10%) served as control.Results: Sperm motility, reactive oxygen species production, across some reaction and deoxyribonucleic acid (DNA) fragmentation decreased significantly, particularly at the highest concentration (405 μg/mL; p 0.001). A substantial increase in the percentage of viable spermatozoa and those with intact mitochondrial membrane potential (MMP) were observed (p 0.001).Conclusion: Aqueous extract of green tea prolonged sperm viability and MMP while reducing sperm intracellular ROS production, capacitation and across some reaction and DNA fragmentation, and may be attributed to its antioxidant potential. However, a high concentration of the extract appears to be detrimental to the functioning of human spermatozoa.

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In vitro effects of aqueous extract of fermented rooibos (Aspalathus linearis) on human sperm function

Cited by 7 publications

References 59 publications

Black Tea Aqueous Extracts Improve Human Sperm Functions: An In Vitro Study

Black Tea Aqueous Extracts Improve Human Sperm Functions: An In Vitro Study

Role of oxidative stress in male infertility

Green tea (Camellia sinensis) aqueous extract improved human spermatozoa functions in vitro

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