In vitro effects of aqueous extract of unfermented rooibos on human spermatozoa

Takalani, Ndivhuho Beauty; Adefolaju, Gbenga Anthony; Henkel, Ralf; Opuwari, Chinyerum Sylvia

doi:10.1111/and.14452

Cited by 4 publications

(4 citation statements)

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“…The washed samples were incubated with black tea aqueous extract (0.4, 4.0, 40, and 405 μg/ml) for 1 hr at 37°C. Additional sperm parameters, sperm motility, vitality, mitochondrial membrane potential (MMP), ROS production, DNA fragmentation, capacitation, and acrosome reaction, were analyzed as previously described [32,[35][36][37][38]. The control contained only HTF-BSA.…”

Section: Methodsmentioning

confidence: 99%

“…The chlortetracycline fluorescence assay protocol was utilized to evaluate the capacitation and acrosome reaction [40]. Using a fluorescence microscope with a 488 nm excitation filter (Zeiss, Oberkochen, Germany), 200 spermatozoa were assessed based on the presence or absence of fluorescence and classified as acrosome reacted, capacitated acrosome intact or noncapacitated, acrosome intact cell [32,38].…”

Section: Capacitation and Acrosome Reaction In Spermmentioning

confidence: 99%

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Black Tea Aqueous Extracts Improve Human Sperm Functions: An In Vitro Study

Setumo,

Choma,

Henkel

et al. 2023

Andrologia

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Infertility affects about 25% of couples worldwide, and oxidative stress (OS) is linked to its idiopathic etiology. Green, black, white, and oolong teas are produced from Camellia sinensis, depending on their oxidation level, and contain antioxidant properties that may enhance male reproductive functions. The study aimed to investigate the effects of black tea aqueous extract on human sperm functions in vitro. Semen samples were collected from donors, liquefied, analyzed, and divided into normal (n = 40) and abnormal (n = 19) groups using the World Health Organization 2010 criteria. Samples were washed and incubated with black tea aqueous extracts (0, 0.4, 4, 40, 405 μg/ml) for 1 hr and analyzed. Along with a considerable decrease in intracellular reactive oxygen species (ROS) production, DNA-fragmented spermatozoa, and acrosome reaction, the percentage of sperm vitality and intact mitochondrial membrane potential (MMP) increased (p<0.05). Furthermore, compared to the normal group, a substantial increase in the percentage of acrosome reaction, ROS production, and percentage of spermatozoa with fragmented DNA, while a reduction in the percentage of intact MMP and sperm vitality, was noted in the abnormal group (p<0.05). Compared to the controls, there was no significant change in motility between the normal and abnormal groups (p>0.05). Black tea’s antioxidant activity, caffeine concentration, or both may have contributed to its improvement in human sperm function in vitro.

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Section: Methodsmentioning

confidence: 99%

Section: Capacitation and Acrosome Reaction In Spermmentioning

confidence: 99%

Black Tea Aqueous Extracts Improve Human Sperm Functions: An In Vitro Study

Setumo,

Choma,

Henkel

et al. 2023

Andrologia

Self Cite

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“…Other compounds such as Cissampelos capensis rhizome aqueous extract [246] and unfermented rooibos [247] seem to induce sperm capacitation in vitro. Some researchers have analysed the effects of phytocomplexes on sperm DNA [238,240,244,245,248].…”

Section: Ellagic Acidmentioning

confidence: 99%

Human Sperm as an In Vitro Model to Assess the Efficacy of Antioxidant Supplements during Sperm Handling: A Narrative Review

Moretti,

Signorini,

Corsaro

et al. 2023

Antioxidants

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Spermatozoa are highly differentiated cells that produce reactive oxygen species (ROS) due to aerobic metabolism. Below a certain threshold, ROS are important in signal transduction pathways and cellular physiological processes, whereas ROS overproduction damages spermatozoa. Sperm manipulation and preparation protocols during assisted reproductive procedures—for example, cryopreservation—can result in excessive ROS production, exposing these cells to oxidative damage. Thus, antioxidants are a relevant topic in sperm quality. This narrative review focuses on human spermatozoa as an in vitro model to study which antioxidants can be used to supplement media. The review comprises a brief presentation of the human sperm structure, a general overview of the main items of reduction–oxidation homeostasis and the ambivalent relationship between spermatozoa and ROS. The main body of the paper deals with studies in which human sperm have been used as an in vitro model to test antioxidant compounds, including natural extracts. The presence and the synergic effects of different antioxidant molecules could potentially lead to more effective products in vitro and, in the future, in vivo.

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“…Slides were then evaluated under a fluorescent microscope (Zeiss, Oberkochen, Germany; 100×) with an excitation and emission filter of 488/520 nm. Two hundred spermatozoa were assessed for the following CTC staining patterns: non-capacitated, acrosome-intact, capacitated, acrosome-intact and capacitated, and acrosome-reacted (Takalani et al 2022).…”

Section: Assessment Of Sperm Capacitation and Acrosome Reactionmentioning

confidence: 99%

Green tea (Camellia sinensis) aqueous extract improved human spermatozoa functions in vitro

Setumo

Choma

Henkel

et al. 2022

JOMPED

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Background: Idiopathic causes of infertility is associated with oxidative stress. Antioxidants are known to scavenge the excessive production of reactive oxygen species (ROS). Green tea (Camellia sinensis) contains polyphenols that enhance its antioxidant potential.Aim: This study focused on the impact of aqueous green tea extract on normozoospermic human spermatozoa.Setting: Department of Medical Biosciences, University of the Western Cape (UWC), South Africa.Methods: Semen samples obtained using masturbation method following three to five days of sexual abstinence from consenting men (n = 59) at the University of the Western Cape (UWC) were liquefied and analysed. Normozoospermic samples were selected according to the World Health Organization (WHO) 5th guideline. Thereafter, semen samples (7.5 × 106 /mL) were washed in human tubular fluid (HTF; 10 min at 300 ×g) and exposed to aqueous extracts of green tea (0 μg/mL, 0.4 μg/mL, 4 μg/mL, 40 μg/mL, 405 μg/mL) for 1 h with various sperm parameters analyzed. Human tubular fluid supplemented with bovine serum albumin (HTF-BSA; 10%) served as control.Results: Sperm motility, reactive oxygen species production, across some reaction and deoxyribonucleic acid (DNA) fragmentation decreased significantly, particularly at the highest concentration (405 μg/mL; p 0.001). A substantial increase in the percentage of viable spermatozoa and those with intact mitochondrial membrane potential (MMP) were observed (p 0.001).Conclusion: Aqueous extract of green tea prolonged sperm viability and MMP while reducing sperm intracellular ROS production, capacitation and across some reaction and DNA fragmentation, and may be attributed to its antioxidant potential. However, a high concentration of the extract appears to be detrimental to the functioning of human spermatozoa.

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In vitro effects of aqueous extract of unfermented rooibos on human spermatozoa

Cited by 4 publications

References 57 publications

Black Tea Aqueous Extracts Improve Human Sperm Functions: An In Vitro Study

Black Tea Aqueous Extracts Improve Human Sperm Functions: An In Vitro Study

Human Sperm as an In Vitro Model to Assess the Efficacy of Antioxidant Supplements during Sperm Handling: A Narrative Review

Green tea (Camellia sinensis) aqueous extract improved human spermatozoa functions in vitro

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