In vitro effects of beta-casomorphins on ion transport in rabbit ileum

Hautefeuille, M; Brantl, Victor; Dumontier, A. M.; Desjeux, J. F.

doi:10.1152/ajpgi.1986.250.1.g92

Cited by 9 publications

(8 citation statements)

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“…8-casomorphins modulate secretion of postprandial insulin (5) and somatostatin (6) in dogs and can elicit analgesia (4). In addition, these peptides alter active intestinal electrolyte transport (17). Whether these peptides cross from blood to cerebrospinal fluid depends on the permeability of the blood brain bamer and on transport mechanisms (19,20).…”

Section: Discussionmentioning

confidence: 99%

Plasma β-Casomorphin-7 Immunoreactive Peptide Increases after Milk Intake in Newborn but not in Adult Dogs

et al. 1989

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ABSTRACT. To determine the relation between milk intake and plasma levels of P-casomorphins as a function of age, we studied the levels of P-casomorphin-7 immunoreactive material (BCMIR) in 2-and 4-wk-old beagle neonates and adult dogs following milk intake. We used three kinds of milk: 1) bovine casein-based milk formula; 2) canine bitch's milk, and 3) soy protein-based (noncasein) formula. Using a new extraction technique, BCMIR was isolated from plasma and assayed by radioimmunoassay at 0, 2, 4, and 6 h after feeding. We found that BCMIR increased significantly in both 2-and 4-wk-old puppies post bovine and canine milk feeding, but not after soy protein formula. Base-line values were about 80-120 fmoll ml and increased to 100-120% 2 h after bovine casein intake in the older puppies and by 4 h in the younger group. BCMIR levels were undetectable in adult dogs before or after bovine milk feeding. 0-casomorphin-7 added to plasma was rapidly degraded whereas BCMIR was stable. Chromatographic profiles demonstrated that the BCMIR moiety is larger than ,f3-casomorphin-7. Our data suggest that the appearance of BCMIR in the plasma of neonatal dogs is a result of P-casein degradation along a permeable gastrointestinal mucosa. We speculate that morphiceptin and P-casomorphins can be cleaved from BCMIR and may have important physiologic effects. (Pediatv Res 26: 34-38, 1989) Abbreviations BCMIR, P-casomorphin-7 immunoreactive material TFA, trifluoroacetic acid Recently, peptide fragments of p-casein, a major milk protein, have been shown to possess opioid activity (1). These peptides, known as (3-casomorphins and morphiceptin, exhibit selective popioid receptor activity like that of morphine (2, 3). For example, p-casomorphins have been shown to induce analgesia (4), influence pancreatic function ( 5 , 6), depress cardiorespiratory function (7), and alter behavior (8).Even though the mechanism of cleavage of this peptide from

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Section: Discussionmentioning

confidence: 99%

Plasma β-Casomorphin-7 Immunoreactive Peptide Increases after Milk Intake in Newborn but not in Adult Dogs

et al. 1989

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“…L'autre action connue des opiacés sur les fonctions intestinales est leur effet sur le transport des électrolytes à ce niveau. Des données particulièrement significatives quant aux éventuelles activités des exorphines du lait sur ces mécanismes ont été apportées par Hautefeuille et al (1986) et Tomé et al (1987). A partir d'expérimentations effectuées in vitro sur l'iléon de lapin, il a été mis en évidence que les j3-casomorphines naturelles et leurs analogues augmentaient, comme les enképhalines, l'absorption des électro-lytes et avaient, de ce fait, une action antidiarrhéique potentielle.…”

Section: Peptides à Activité Opiacéeunclassified

Peptides du lait à activité biologique

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1989

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“…h-'/cm2) and stimulated net Na the nonmetabolized analogue ,Ll-[~Ala~,~,Tyr~] CM-5-NHz, whose and C1 absorption to the same extent. In CT-treated ileum, sequence is Tyr-~Ala-Phe-~Ala-Tyr-NHz, acts on electrolyte both serosal M) and mucosal (5.10-4 M) addition of transport from both the mucosal and serosal sides of the epithethe analogue did not further modify the rise in Isc caused lium in vitro (7,8). by CT but also stimulated net Na and C1 absorption.…”

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confidence: 87%

Luminal Antisecretory Effects of a β-Casomorphin Analogue on Rabbit Ileum Treated with Cholera Toxin

et al. 1988

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Because the physiologic significance of the presence of the opioid peptides beta-casomorphins (beta-CMs) in enzymatic digestion of milk proteins is still undetermined, the effect of the nonmetabolized beta-CM analogue beta-[DAla2,4,Tyr5]CM-5-NH2 on water and electrolyte movements was studied in vivo and in vitro in rabbit ileum untreated or treated with cholera toxin (CT). When this analogue was introduced in vivo at a concentration of 10(-3) M into the lumen of rabbit ileal loops, it significantly stimulated net water absorption in untreated loops and reduced net water secretion in CT-treated loops. In vitro addition of this analogue (10(-4) M) to the serosal compartment of untreated ileum in an Ussing chamber reduced Isc (delta Isc = 0.44 +/- 0.05 muEq.h-1/cm2) and stimulated net Na and Cl absorption to the same extent. In CT-treated ileum, both serosal (10(-4) M) and mucosal (5.10(-4) M) addition of the analogue did not further modify the rise in Isc caused by CT but also stimulated net Na and Cl absorption. On the mucosal side, the effect of the analogue was accompanied by its transfer from the luminal to the blood side of the tissue. The transferred analogue was intact as shown by HPLC (Jm----s = 2.4 +/- 0.8 nmol.h-1/cm2). These results demonstrated that the beta-CM analogue stimulates intestinal absorption of electrolytes in rabbit ileum both in the basal state and after its stimulation by CT.(ABSTRACT TRUNCATED AT 250 WORDS)

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In vitro effects of beta-casomorphins on ion transport in rabbit ileum

Cited by 9 publications

References 0 publications

Plasma β-Casomorphin-7 Immunoreactive Peptide Increases after Milk Intake in Newborn but not in Adult Dogs

Plasma β-Casomorphin-7 Immunoreactive Peptide Increases after Milk Intake in Newborn but not in Adult Dogs

Peptides du lait à activité biologique

Luminal Antisecretory Effects of a β-Casomorphin Analogue on Rabbit Ileum Treated with Cholera Toxin

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