In vitro effects of l-carnitine and glutamine on motility, acrosomal abnormality, and plasma membrane integrity of rabbit sperm during liquid-storage

Sarıözkan, Savaş; Özdamar, Saim; Türk, Gaffari; Cantürk, Fazile; Yay, Arzu

doi:10.1016/j.cryobiol.2014.04.006

Cited by 36 publications

(33 citation statements)

References 31 publications

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“…The cryopreservation of semen includes the decrease in temperature and increase in oxidative stress on the sperm membrane which results in irreversible damage to intracellular structures and changes in enzymatic activity and associated reduction in motility, fertilizing ability of spermatozoa [ 25 ]. Amino acids are the constituents of the seminal plasma and have been used in different combinations in extenders for the cryopreservation of semen in different livestock species [ 12 , 18 , 26 ] by virtue of their membrane protecting ability. In the present experiment, the supplementation of glutamine to TE based extender improved the spermatozoa quality with respect to the progressive motility, percent live spermatozoa and acrosomal damage both after the equilibration and post-thaw.…”

Section: Discussionmentioning

confidence: 99%

“…Glutamate is formed in a substantial amount of seminiferous tubules [ 13 ] and constitutes 90% of total amino acids of rete-testes fluid [ 14 ]. Amongst the amino acids used in freezing extender, glutamine/glutamic acid has been reported to be used as an additive in several species such as angora goat [ 15 ], ram [ 16 ], donkey [ 17 ], rabbit [ 18 ] and bull [ 19 , 20 ] spermatozoa against freeze-thawing damage. The cryoprotective nature of glutamine is attributed to its anti-oxidative property which is responsible for improved post-thaw motility pattern and plasma membrane integrity of spermatozoa [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

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Effect of glutamine supplementation and replacement of tris-egg yolk based extender with defatted cow milk on spermatozoa quality after equilibration and thawing

Dawra¹,

Yadav²,

Yadav³

2015

Vet World

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Aim:The present study was designed to evaluate the effect of supplementation of glutamine and replacement of Tris-egg yolk (TE) based buffer with defatted cow milk on the spermatozoa quality after equilibration and thawing.Materials and Methods:Semen was collected from five Bhadawari bulls biweekly, and a total of 30 ejaculates were taken. The semen ejaculates were pooled and divided into three equal parts. The pooled semen was diluted by TE based extender (control), TE + glutamine (8 mM) (T1) and 50% TE + 50% deffated cow milk + glutamine (8 mM) (T2). At two stages viz. after equilibration and after 12 h of cryopreservation (thawed samples), progressive motility, percent live, and percent acrosomal damage of the spermatozoa was assessed.Results:Supplementation of glutamine improved (p<0.05) the spermatozoa quality with respect to the progressive motility, percent live and acrosomal damage both post-equilibration and post-thaw. T2 improved (p<0.05) the spermatozoa quality as compared to control, however; it was less (p<0.05) effective as compared T1 both post-equilibration and post-thaw.Conclusion:From the results of present study it can be concluded that glutamine supplementation was effective in maintaining post-equilibration and post-thaw spermatozoa quality whereas defatted cow milk was not as effective as TE based buffer in the extender in improving the spermatozoa quality.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect of glutamine supplementation and replacement of tris-egg yolk based extender with defatted cow milk on spermatozoa quality after equilibration and thawing

Dawra¹,

Yadav²,

Yadav³

2015

Vet World

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“…The cooling of sperm in liquid suspension slows metabolism, increases sperm survival and maintain sperm fertility (KANEKO et al, 2009), but the storage time has effects on sperm motility and viability (SALAMON & MAXWELL, 2000;SARIÖZKAN et al, 2014). BERGSTEIN-GALAN et al (2016) reported that TM of ovine semen collected in an AV decreased at 48 hours after the start of refrigeration at 5°C.…”

Section: Discussionmentioning

confidence: 99%

Ovine epididymal spermatozoa preservation in liquid state with or without seminal plasma epididimários ovinos

et al. 2018

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Preservation and use of spermatozoa that have been recovered after death can extend the use of genetically superior animals. The objective of this study was to evaluate the maximum period for which ovine spermatozoa could be successfully stored in refrigerated dilution medium post-mortem, with or without added seminal plasma. Three samples of spermatozoa collected in an artificial vagina from 10 rams, or from the tails of four epididymes from the same rams at the time of death (G0) and six (G6), twelve (G12), twenty-four (G24) and forty-eight (G48) hours after death were used. After recovery, the spermatozoa were refrigerated at 5°C in either control medium (CM) or control medium plus 20%homologous seminal plasma (SP) and evaluated for 72 hours from the start of refrigeration. The G48 samples had a lower(P <0.05) total motility (TM) and plasma membrane integrity in the hyposmotic test (HOST) than the other groups evaluated at all analyzed times. The TM decreased (P <0.05) after 24 hours of cooling in semen collected in AV, at G0 and G24 and after 48 hours of refrigeration in G6 and G12. The TM and HOST integrity and sperm morphology did not differ between samples refrigerated in CM or SP. In conclusion, it is possible to collect epididymal spermatozoa up to 24 hours after death. Sperm viability can be prolonged fora further 48 hours by refrigeration. However, total motility decreases from 24 hours after refrigeration and the supplementation of 20% seminal plasma to the extender has no effect on spermatozoa longevity.

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“…In some studies, it has been alleged that amino acids such as carnitine, glutamine (Sarıozkan, Ozdamar, Turk, Canturk, & Yay, ), albumin and cysteine (Uysal & Bucak, ) protect spermatozoa against motility losses and membrane disorders during cryopreservation. l ‐arginine has been reported to inhibit lipid peroxidation and support the antioxidant defence mechanism by inducing NO and decreasing ROS (Shan, Wang, Gao, Cao, & Zhang, ).…”

Section: Discussionmentioning

confidence: 99%

Effect ofl-arginine addition on long-term storability of ram semen

2017

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This study was conducted to investigate the effect of l-arginine addition on long-term storability of ram semen. Six Akkaraman rams were used as material. Semen samples were collected. Pooled samples were diluted and were divided into six equal aliquots. While aliquot 1 was kept as control, the stock solutions including 0.1, 0.5, 1, 5 and 10 mm l-arginine were added to other aliquots. All aliquots were routinely frozen in 0.25-ml straws at -130°C liquid nitrogen vapour and stored in liquid nitrogen -196°C until being analysed. The equilibrated and thawed sperm motility, membrane integrity and arginase activity were evaluated. While the 10 mm l-arginine supplementation significantly (p < .001) decreased equilibrated sperm motility, the 5 mm significantly (p < .05) increased the membrane integrity and arginase activity in comparison with the control group. The motility (p < .001) and membrane integrity (p < .01) were determined to be highest in 0.5 mm group, while significant reductions were observed in motility (p < .001) of 10 mm group and arginase activity (p < .05) of 1, 10 mm groups as compared to the control group. It was concluded that in vitro addition of 0.5 mm l-arginine to ram semen may be useful, but 10 mm may be harmful to spermatozoa quality during long-term storage.

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In vitro effects of l-carnitine and glutamine on motility, acrosomal abnormality, and plasma membrane integrity of rabbit sperm during liquid-storage

Cited by 36 publications

References 31 publications

Effect of glutamine supplementation and replacement of tris-egg yolk based extender with defatted cow milk on spermatozoa quality after equilibration and thawing

Effect of glutamine supplementation and replacement of tris-egg yolk based extender with defatted cow milk on spermatozoa quality after equilibration and thawing

Ovine epididymal spermatozoa preservation in liquid state with or without seminal plasma epididimários ovinos

Effect ofl-arginine addition on long-term storability of ram semen

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