In vitro effects of nitrogen dioxide on the release of nitric oxide by bovine alveolar macrophages

Höckele, V; Krüger, Evelyn; Krug, Harald F.; Seidel, A.

doi:10.1016/s0378-4274(98)00050-2

Cited by 6 publications

(2 citation statements)

References 17 publications

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“…As this is a challenging technique, only a few systems are described in the literature, which permit this type of exposure to be achieved in studies on the effects of gases or aerosols. In the classical method, lung cells are placed on special holding devices in temperature-controlled exposure chambers flushed with the aerosol (3)(4)(5)(6).…”

Section: Introductionmentioning

confidence: 99%

The Response of a Co-culture Lung Model to Fine and Ultrafine Particles of Incinerator Fly Ash at the Air–liquid Interface

Diabaté¹,

Mülhopt²,

Paur³

et al. 2008

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Elevated concentrations of particulate matter in the environmental atmosphere constitute a potential risk to human health. In vitro cell-based assays are therefore necessary to evaluate the toxicological potential of inhaled particulate emissions. In this study, the exposure of a co-culture cell model at the air-liquid interface was used to evaluate the dose-dependent biological effects of a test aerosol. The CUL-TEX ® system was used to expose human cells to an environmentally-relevant aerosol, generated from fly ash collected in a commercial municipal waste incinerator and resuspended in filtered air. Human bronchial epithelial cells, BEAS-2B, co-cultured with differentiated THP-1 macrophages growing on Transwell ® inserts, were employed in the bioassay. Analyses of cell viability, interleukin-8 (IL-8) release, intracellular glutathione, and haeme oxygenase-1 enzyme expression were performed. Transportation of the cells and exposure to humidified filtered air or the test aerosol, at 100ml/min for 1 to 6 hours, were well tolerated by the cells and had no effect on their viability. Levels of IL-8 release and haeme oxygenase-1 expression were elevated by exposure to fly ash aerosol as a function of time, but not by exposure to clean air. For IL-8 release, a dose-dependent effect was demonstrated with the assumption that the deposited mass of the particles correlated with exposure time. Exposure to the test aerosol did not affect the intracellular glutathione concentration. This in vitro approach simulates particle deposition in the human lung more realistically than does submerged exposure, and it preserves the inherent properties of the particles. It shows promise for use to detect particulate emissions which are potentially detrimental to human health.

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Section: Introductionmentioning

confidence: 99%

The Response of a Co-culture Lung Model to Fine and Ultrafine Particles of Incinerator Fly Ash at the Air–liquid Interface

Diabaté¹,

Mülhopt²,

Paur³

et al. 2008

Altern Lab Anim

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“…1). The Voisin chamber has been used to study the effects of ozone (Arsalane et al, 1995), nitrogen dioxide (Wallaert and Voisin, 1992) and tobacco smoke (Dubar et al, 1993) with alveolar macrophages obtained by bronchoalveolar lavage from humans and animals, of ozone with human BEAS-2B epithelial cells (Mögel et al, 1998), of NO 2 with primary bovine alveolar macrophages and murine RAW-264.7 macrophages (Höckele et al, 1998). Some laboratories used designs similar to the Voisin system, e.g.…”

Section: Exposure At the Air-liquid Interfacementioning

confidence: 99%

In Vitro Exposure Systems and Bioassays for the Assessment of Toxicity of Nanoparticles to the Human Lung

et al. 2008

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The rapid development of nanotechnology requires the production of nanoparticles which are found in numerous novel products. To reduce the number of animal tests during the assessment of lung toxicity of airborne nanoparticles in vitro exposure systems and lung specific bioassays have been developed. The reproducible application of bioassays for exposure of lung cells at the air-liquid interface promises a higher efficiency and cost reductions in toxicological testing. Despite significant progress of the exposure technology and fundamentals of bioassays a stringent validation of the in vitro versus in vivo tests is still lacking.

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In vitro genotoxicity assay of sidestream smoke using a human bronchial epithelial cell line

Wolz

Krause

Scherer

et al. 2002

Food and Chemical Toxicology

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In vitro effects of nitrogen dioxide on the release of nitric oxide by bovine alveolar macrophages

Cited by 6 publications

References 17 publications

The Response of a Co-culture Lung Model to Fine and Ultrafine Particles of Incinerator Fly Ash at the Air–liquid Interface

The Response of a Co-culture Lung Model to Fine and Ultrafine Particles of Incinerator Fly Ash at the Air–liquid Interface

In Vitro Exposure Systems and Bioassays for the Assessment of Toxicity of Nanoparticles to the Human Lung

In vitro genotoxicity assay of sidestream smoke using a human bronchial epithelial cell line

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