In vitro effects of selected contact lens care solutions on Acanthamoeba castellanii strains in Poland

Padzik, Marcin; Chomicz, Lidia; Szaflik, Jacek P.; Chruścikowska, Agnieszka; Perkowski, Konrad; Szaflik, Jerzy

doi:10.1016/j.exppara.2014.06.014

Cited by 26 publications

(39 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Among the risk factors for AK, apart from contact lens use, the corneal trauma as well as poor hygiene in the handling of contact lenses and their cases are listed [1]. In addition, a low activity against Acanthamoeba has been reported in most of the commonly used contact lens solutions [10,11,24,25]. This last observation was confirmed by the results of the current study, since none of the tested contact lens solutions showed amoebicidal activity.…”

Section: Discussionsupporting

confidence: 82%

“…Previous studies have shown that the most popular multipurpose contact lens disinfection systems, commonly based on anti-microbial and anti-fungal agents, are not fully effective against Acanthamoeba. Therefore, there is an urgent need to broaden research in order to develop contact lens solutions with improved anti-amoebic activity [10,11,12].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Evaluation of in vitro effect of selected contact lens solutions conjugated with nanoparticles in terms of preventive approach to public health risk generated by Acanthamoeba strains

Padzik¹,

Hendiger²,

Żochowska³

et al. 2019

Ann Agric Environ Med.

Self Cite

View full text Add to dashboard Cite

Introduction. Various Acanthamoeba species are free-living organisms widely distributed in the human environment. Amphizoic amoebae as facultative parasites may cause vision-threatening eye disease-Acanthamoeba keratitis, mostly among contact lens wearers. As the number of cases is increasing, and applied therapy often unsuccessful, proper hygienic measures and effective contact lenses disinfection are crucial for the prevention of this disease. Available contact lens solutions are not fully effective against amphizoic amoebae; there is a need to enhance their disinfecting activity to prevent amoebic infections. The use of developing nanotechnology methods already applied with success in the prevention, diagnostic and therapy of other infectious diseases might be helpful regarding amoebic keratitis. This study assesses the in vitro effect of selected contact lens solutions conjugated with nanoparticles against Acanthamoeba trophozoites. Materials and method. Three selected contact lens solutions conjugated with silver and gold nanoparticles in concentration of 0.25-2.5 ppm were used in vitro against the axenically cultured ATCC 30010 type Acanthamoeba castellanii strain. The anti-amoebic efficacy was examined based on the oxido-reduction of AlamarBlue. The cytotoxicity tests based on the measurement of lactate dehydrogenase (LDH) activity were performed using a fibroblast HS-5 cell line. Results. Enhancement of the anti-amoebic activity of contact lens solutions conjugated with selected nanoparticles expressed in the dose dependent amoebic growth inhibition with a low cytotoxicity profile was observed. Conclusions. Results of the study showed that conjugation of selected contact lens solutions with silver nanoparticles might be a promising approach to prevent Acanthamoeba keratitis among contact lens users.

show abstract

Section: Discussionsupporting

confidence: 82%

Section: Introductionmentioning

confidence: 99%

Evaluation of in vitro effect of selected contact lens solutions conjugated with nanoparticles in terms of preventive approach to public health risk generated by Acanthamoeba strains

Padzik¹,

Hendiger²,

Żochowska³

et al. 2019

Ann Agric Environ Med.

Self Cite

View full text Add to dashboard Cite

show abstract

“…In this study, we tested multipurpose contact lens disinfecting systems containing different active ingredients but characterized by similar mode of action, resulting in cell membrane perturbation (Table 1) The obtained results con rmed lack of amoebicidal activity of all tested multipurpose contact lens solutions against Acanthamoeba strain. Our results are in accordance with other publications and showed that disinfecting capabilities of market available contact lens solutions are insu cient [11], [12], [28]- [31].…”

Section: Discussionsupporting

confidence: 93%

Tannic acid-modified silver nanoparticles enhance anti-Acanthamoeba activity without increasing cytotoxicity of the contact lens solutions

Hendiger

Padzik

Żochowska

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

Background: Free living amoebae of Acanthamoeba genus are cosmopolitan, widely distributed protozoans causing severe, vision-threatening corneal infection known as Acanthamoeba keratitis (AK). Majority of the increasing number of AK cases are associated with contact lenses use. Due to lack of effective therapies against AK, proper eye hygiene and effective contact lenses disinfection are crucial in prevention of this infection. Currently available multipurpose contact lens disinfection systems are not fully effective against Acanthamoeba trophozoites and cysts. There is an urgent need to increase the disinfecting activity of these systems to prevent Acanthamoeba keratitis infections. Synthesized nanoparticles have been recently studied and proposed as a new generation of anti-microbial agents. It is also known that plant metabolites, including tannins, present anti-parasitic activity. The aim of this study was to evaluate the anti-amoebic activity and cytotoxicity of the tannic acid-modified silver nanoparticles (AgTANPs) conjugated with selected multipurpose contact lens solutions. Methods: The anti-amoebic activity of pure contact lens care solutions and nanoparticles conjugated with contact lens care solutions were examined in vitro by colorimetric assay, based on the oxido-reduction of AlamarBlue. The cytotoxicity assays were performed using a fibroblast HS-5 (ATCC CRL-11882) cell line. Results were statistically analyzed by ANOVA and Student-Newman-Keuls tests using the p<0.05 level of statistical significance. Results: The obtained results showed that nanoparticles enhanced anti-Acanthamoeba activity of the tested contact lens solutions without increasing their cytotoxicity profile. The activity is enhanced within minimal disinfection time recommended by the manufacturer. Conclusions: The conjugation of selected contact lens solutions with AgTANPs might be a novel and promising approach as part of preventive actions of Acanthamoeba keratitis infections among contact lens users.

show abstract

“…However, none of the contact lens disinfectants completely destroyed A. castellanii trophozoites and cyst. These findings are consistent with several recent studies indicating that marketed contact lens disinfectants are ineffective in completely destroying amoebae (Kolar et al, 2015;Padzik et al, 2014;Polat et al, 2007;Ustüntürk and Zeybek, 2014). In particular, there show limited efficacy against cysts.…”

Section: Discussionsupporting

confidence: 82%

Status of the effectiveness of contact lens disinfectants in Malaysia against keratitis-causing pathogens

Abjani

Khan

Jung

et al. 2017

Experimental Parasitology

View full text Add to dashboard Cite

The aim of this study was (i) to assess the antimicrobial effects of contact lens disinfecting solutions marketed in Malaysia against common bacterial eye pathogens and as well as eye parasite, Acanthamoeba castellanii, and (ii) to determine whether targeting cyst wall would improve the efficacy of contact lens disinfectants. Using ISO 14729 Stand-Alone Test for disinfecting solutions, bactericidal and amoebicidal assays of six different contact lens solutions including Oxysept ® , AO SEPT PLUS, OPTI-FREE ® pure moist ® , Renu ® fresh™, FreshKon ® CLEAR and COMPLETE RevitaLens™ were performed using Manufacturers Minimum recommended disinfection time (MRDT). The efficacy of contact lens solutions was determined against keratitis-causing microbes, namely: Pseudomonas aeruginosa, Methicillin-resistant Staphylococcus aureus, Streptococcus pyogenes, Streptococcus pneumoniae, and Acanthamoeba castellanii. In addition, using chlorhexidine as an antiamoebic compound and cellulase enzyme to disrupt cyst wall structure, we determined whether combination of both agents can enhance efficacy of marketed contact lens disinfectants against A. castellanii trophozoites and cysts, in vitro. The results revealed that all contact lens disinfectants tested showed potent bactericidal effects exhibiting 100% kill against all bacterial species tested. In contrast, none of the contact lens disinfectants had potent effects against Acanthamoeba cysts viability. When tested against trophozoites, two disinfectants, Oxysept Multipurpose and AO-sept Multipurpose showed partial amoebicidal effects. Using chlorhexidine as an antiamoebic compound and cellulase enzyme to disrupt cyst wall structure, the findings revealed that combination of both agents in contact lens disinfectants abolished viability of A. castellanii cysts and trophozoites. Given the inefficacy of contact lens disinfectants tested in this study, these findings present a significant concern to public health. These findings revealed that targeting cyst wall by using cyst wall degrading molecules in contact lens disinfecting solutions will enhance their efficacy against this devastating eye infection.

show abstract

In vitro effects of selected contact lens care solutions on Acanthamoeba castellanii strains in Poland

Cited by 26 publications

References 31 publications

Evaluation of in vitro effect of selected contact lens solutions conjugated with nanoparticles in terms of preventive approach to public health risk generated by Acanthamoeba strains

Evaluation of in vitro effect of selected contact lens solutions conjugated with nanoparticles in terms of preventive approach to public health risk generated by Acanthamoeba strains

Tannic acid-modified silver nanoparticles enhance anti-Acanthamoeba activity without increasing cytotoxicity of the contact lens solutions

Status of the effectiveness of contact lens disinfectants in Malaysia against keratitis-causing pathogens

Contact Info

Product

Resources

About