In Vitro Effects of Short-Term and Long-Term Heat Exposures on the Immune Response and Prostaglandin Biosynthesis in Bovine Endometrial Cells

Chotimanukul, Sroisuda; Suwimonteerabutr, Junpen; Techakumphu, Mongkol; Swangchan-Uthai, Theerawat

doi:10.3390/ani12182359

Cited by 4 publications

(2 citation statements)

References 71 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It has been proposed to be involved in human platelet aggregation as well chemotactic neutrophil migration [ 36 , 37 ]. Furthermore, a study, which investigated the immune response of the cow endometrium to heat stress, reported an increased expression ALOX12 in the group exposed to long-term heat stress [ 38 ]. TRAPPC9 gene is known to play a key role in the NF-κB transcription factor, which involved a number of cellular processes such as innate and adaptive immunity, cellular proliferation, apoptosis, and development [ 39 ].…”

Section: Discussionmentioning

confidence: 99%

The transcriptomic response of bovine uterine tissue is altered in response to sperm from high- and low-fertility bulls

Donnellan

Cormican

Reid³

et al. 2023

Biology of Reproduction

View full text Add to dashboard Cite

Despite stringent quality control checks, some bulls with apparently normal semen quality yield lower than expected pregnancy rates. This study profiled the transcriptome and performed histological analysis of the bovine uterus in response to sperm from high fertility (HF) and low fertility (LF) bulls. Post-mortem uterine biopsies and uterine explants were collected from heifers 12 h after a fixed time artificial insemination (AI) to a synchronised oestrus with frozen–thawed semen from 5 HF (fertility rate 4.01% ± 0.25) and 5 LF (fertility rate − 11.29% ± 1.11; mean ± s.e.m.) bulls. Uterine biopsies were also collected from control (CTRL) heifers which were not inseminated. RNA-sequencing and histological analysis were performed for differential gene expression and neutrophil quantification. In the HF treatment relative to CTRL heifers there were 376 genes significantly differentially expressed in the endometrium with just one gene differentially expressed in the LF treatment relative to CTRL heifers. Comparing the HF and LF treatments directly there were 40 significantly differentially expressed genes (DEG; P < 0.05). Transcriptomic analysis shows a predominant role for the inflammatory marker Interleukin-1 alpha, which was further confirmed by immunohistochemistry. Quantification of neutrophils in the endometrium showed a significant effect of sperm, however, there was no difference in neutrophil numbers between HF and LF groups. In conclusion, this novel study clearly shows a distinct inflammatory response to sperm in the endometrium and a divergent transcriptomic response to semen from HF and LFbulls.

show abstract

Section: Discussionmentioning

confidence: 99%

The transcriptomic response of bovine uterine tissue is altered in response to sperm from high- and low-fertility bulls

Donnellan

Cormican

Reid³

et al. 2023

Biology of Reproduction

View full text Add to dashboard Cite

show abstract

“…Specifically, granulosa cells responsible for estradiol production are adversely affected by HS, resulting in decreased estradiol production and subsequent disruption of the estrous cycle in cattle [ 59 , 60 , 61 ]. HS has also been shown to influence corticoid levels, luteinizing hormone (LH), and plasma progesterone in cattle, with alterations in hypothalamus and pituitary gland function leading to changes in the secretion of reproductive hormones [ 9 , 62 , 63 , 64 , 65 , 66 ]. Moreover, HS negatively impacts oocyte quality due to its effects on the hypothalamus and pituitary gland, specifically the LH [ 67 , 68 , 69 ].…”

Section: Introductionmentioning

confidence: 99%

Advancements in Genetic Biomarkers and Exogenous Antioxidant Supplementation for Safeguarding Mammalian Cells against Heat-Induced Oxidative Stress and Apoptosis

Khan,

Chen

et al. 2024

Antioxidants

View full text Add to dashboard Cite

Heat stress represents a pervasive global concern with far-reaching implications for the reproductive efficiency of both animal and human populations. An extensive body of published research on heat stress effects utilizes controlled experimental environments to expose cells and tissues to heat stress and its disruptive influence on the physiological aspects of reproductive phenotypic traits, encompassing parameters such as sperm quality, sperm motility, viability, and overall competence. Beyond these immediate effects, heat stress has been linked to embryo losses, compromised oocyte development, and even infertility across diverse species. One of the primary mechanisms underlying these adverse reproductive outcomes is the elevation of reactive oxygen species (ROS) levels precipitating oxidative stress and apoptosis within mammalian reproductive cells. Oxidative stress and apoptosis are recognized as pivotal biological factors through which heat stress exerts its disruptive impact on both male and female reproductive cells. In a concerted effort to mitigate the detrimental consequences of heat stress, supplementation with antioxidants, both in natural and synthetic forms, has been explored as a potential intervention strategy. Furthermore, reproductive cells possess inherent self-protective mechanisms that come into play during episodes of heat stress, aiding in their survival. This comprehensive review delves into the multifaceted effects of heat stress on reproductive phenotypic traits and elucidates the intricate molecular mechanisms underpinning oxidative stress and apoptosis in reproductive cells, which compromise their normal function. Additionally, we provide a succinct overview of potential antioxidant interventions and highlight the genetic biomarkers within reproductive cells that possess self-protective capabilities, collectively offering promising avenues for ameliorating the negative impact of heat stress by restraining apoptosis and oxidative stress.

show abstract

Impact of Heat Stress on Oocyte Developmental Competence and Pre-Implantation Embryo Viability in Cattle

Gómez-Guzmán,

Parra-Bracamonte,

Velazquez

2024

Animals

View full text Add to dashboard Cite

Rectal and vaginal temperatures are utilised in both in vivo and in vitro models to study the effects of heat stress on oocyte competence and embryo viability in cattle. However, uterine temperature increases by only 0.5 °C in heat-stressed cows, significantly lower than simulated increases in in vitro models. Temperature variations within oviducts and ovarian follicles during heat stress are poorly understood or unavailable, and evidence is lacking that oocytes and pre-implantation embryos experience mild (40 °C) or severe (41 °C) heat stress inside the ovarian follicle and the oviduct and uterus, respectively. Gathering detailed temperature data from the reproductive tract and follicles is crucial to accurately assess oocyte competence and embryo viability under realistic heat stress conditions. Potential harm from heat stress on oocytes and embryos may result from reduced nutrient availability (e.g., diminished blood flow to the reproductive tract) or other unidentified mechanisms affecting tissue function rather than direct thermal effects. Refining in vivo stress models in cattle is essential to accurately identify animals truly experiencing heat stress, rather than assuming heat stress exposure as done in most studies. This will improve model reliability and aid in the selection of heat-tolerant animals.

show abstract

In Vitro Effects of Short-Term and Long-Term Heat Exposures on the Immune Response and Prostaglandin Biosynthesis in Bovine Endometrial Cells

Cited by 4 publications

References 71 publications

The transcriptomic response of bovine uterine tissue is altered in response to sperm from high- and low-fertility bulls

The transcriptomic response of bovine uterine tissue is altered in response to sperm from high- and low-fertility bulls

Advancements in Genetic Biomarkers and Exogenous Antioxidant Supplementation for Safeguarding Mammalian Cells against Heat-Induced Oxidative Stress and Apoptosis

Impact of Heat Stress on Oocyte Developmental Competence and Pre-Implantation Embryo Viability in Cattle

Contact Info

Product

Resources

About