In vitro efficacy of a Hydrophilic Central Venous Catheter Loaded with Silver to Prevent Microbial Colonization

Gatter, N.; Kohnen, Wolfgang; Jansen, B.

doi:10.1016/s0934-8840(98)80162-x

Cited by 34 publications

(19 citation statements)

References 17 publications

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“…However, silver has been used as a microbiocidal agent for over a century and silver products are commonly used in the medical and dental fields (15). Silver-impregnated cloth and topicals containing silver sulfadiazine are used in hospital burn wards, and catheters containing silver as an antiseptic are widely used (9,12,22,27,32,38). C. albicans is a frequent nosocomial infectious agent in burn patients and patients with indwelling catheters.…”

Section: Discussionmentioning

confidence: 99%

Role of aCandida albicansP1-Type ATPase in Resistance to Copper and Silver Ion Toxicity

2000

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Copper ion homeostasis is complicated in that copper is an essential element needed for a variety of cellular processes but is toxic at excess levels. To identify Candida albicans genes that are involved in resistance to copper ion toxicity, a library containing inserts of C. albicans genomic DNA was used to complement the copper sensitivity phenotype of a Saccharomyces cerevisiae cup1⌬ strain that is unable to produce Cup1p, a metallothionein (MT) responsible for high-level copper ion resistance. A P1-type ATPase (CPx type) that is closely related to the human Menkes and Wilson disease proteins was cloned. The gene encoding this pump was termed CRD1 (for copper resistance determinant). A gene encoding a 76-amino-acid MT similar to higher eukaryotic MTs in structure was also cloned, and the gene was termed CRD2. Transcription of the CRD1 gene was found to increase upon growth with increasing copper levels, while the CRD2 mRNA was expressed at a constant level. Strains with the CRD1 gene disrupted were extremely sensitive to exogenous copper and failed to grow in medium containing 100 M CuSO 4 . These crd1 strains also exhibited increased sensitivity to silver and cadmium, indicating that Crd1p is somewhat promiscuous with respect to metal ion transport. Although strains with the CRD2 gene disrupted showed reduced growth rate with increasing copper concentration, the crd2 mutants eventually attained wild-type levels of growth, demonstrating that CRD2 is less important for resistance to copper ion toxicity. Crd1p is the first example of a eukaryotic copper pump that provides the primary source of cellular copper resistance, and its ability to confer silver resistance may enhance the prevalence of C. albicans as a nosocomial pathogen.

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Section: Discussionmentioning

confidence: 99%

Role of aCandida albicansP1-Type ATPase in Resistance to Copper and Silver Ion Toxicity

2000

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“…In vitro studies have shown that silver-impregnated catheters can oppose colonization (17)(18)(19)(20)(21)(22)(23)(24). A new generation of silver-impregnated catheters based on the use of an inorganic silver powder, on which silver ions are bonded with an inert ceramic zeolite (Agion), has become recently available (Vygon, Ecouen, France).…”

mentioning

confidence: 99%

Comparison of silver-impregnated with standard multi-lumen central venous catheters in critically ill patients*

Kalfon

Vaumas

Samba

et al. 2007

Critical Care Medicine

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“…The introduction of Ag 2 O into the bioactive glass composition is aimed at minimizing the risk of microbial contamination through the potential antimicrobial activity of the leaching Ag ϩ ions (7, 13). The introduction of silver has recently become one of the preferred methods to confer microbial resiliency on biomedical materials and devices (1,6,17,18,21), since the incidence of biomaterial-centered infections is one of the main causes of revision surgery (12). The production of the material via the sol-gel process allows the tailoring of the textural characteristics of the matrix in order to obtain a controlled Ag ϩ delivery system.…”

mentioning

confidence: 99%

Broad-Spectrum Bactericidal Activity of Ag ₂ O-Doped Bioactive Glass

Bellantone¹,

Williams

Hench³

2002

Antimicrob Agents Chemother

356

220

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Bioactive glass has found extensive application as an orthopedic and dental graft material and most recently also as a tissue engineering scaffold. Here we report an initial investigation of the in vitro antibacterial properties of AgBG, a novel bioactive glass composition doped with Ag 2 O. The bacteriostatic and bactericidal properties of this new material and of two other bioactive glass compositions, 45S5 Bioglass and BG, have been studied by using Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus as test microorganisms. Concentrations of AgBG in the range of 0.05 to 0.20 mg of AgBG per ml of culture medium were found to inhibit the growth of these bacteria. Not only was AgBG bacteriostatic, but it also elicited a rapid bactericidal action. A complete bactericidal effect was elicited within the first hours of incubation at AgBG concentrations of 10 mg ml ؊1 . 45S5 Bioglass and BG had no effect on bacterial growth or viability. The antibacterial action of AgBG is attributed exclusively to the leaching of Ag ؉ ions from the glass matrix. Analytical measurements rule out any contribution to AgBG-mediated bacterial killing by changes in pH or ionic strength or the dissolution of other ionic species from the biomaterials. Our observations of the dissolution profiles of Ag ؉ from AgBG in the presence and absence of bacteria are consistent with silver accumulation by the bacteria.Bioactive glasses are special glass systems which are generally composed of SiO 2 , CaO, P 2 O 5 , and Na 2 O. They can be produced by the traditional melting process or by the more versatile sol-gel process (14, 15). The bioactive behavior of these glasses is defined as the ability to bond to soft and hard tissues by means of a series of reactions, which produces a strong, compliant interface between the glass and the tissue (14). Due to their high level of tissue integration and regeneration (15, 34), bioactive glasses have been used clinically in a variety of situations. Bioactive glass devices are now available to treat conductive deafness and alveolar ridge resorption and bone loss due to periodontal disease and to fill cystic and surgically created defects, particularly in craniomaxillofacial sites (19,25,33).The material under investigation in the present work is a novel bioactive glass system composed of SiO 2 , CaO, P 2 O 5 , and Ag 2 O. The introduction of Ag 2 O into the bioactive glass composition is aimed at minimizing the risk of microbial contamination through the potential antimicrobial activity of the leaching Ag ϩ ions (7, 13). The introduction of silver has recently become one of the preferred methods to confer microbial resiliency on biomedical materials and devices (1,6,17,18,21), since the incidence of biomaterial-centered infections is one of the main causes of revision surgery (12). The production of the material via the sol-gel process allows the tailoring of the textural characteristics of the matrix in order to obtain a controlled Ag ϩ delivery system. Here we report an initial investigation of ...

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In vitro efficacy of a Hydrophilic Central Venous Catheter Loaded with Silver to Prevent Microbial Colonization

Cited by 34 publications

References 17 publications

Role of aCandida albicansP1-Type ATPase in Resistance to Copper and Silver Ion Toxicity

Role of aCandida albicansP1-Type ATPase in Resistance to Copper and Silver Ion Toxicity

Comparison of silver-impregnated with standard multi-lumen central venous catheters in critically ill patients*

Broad-Spectrum Bactericidal Activity of Ag ₂ O-Doped Bioactive Glass

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In vitro efficacy of a Hydrophilic Central Venous Catheter Loaded with Silver to Prevent Microbial Colonization

Cited by 34 publications

References 17 publications

Role of aCandida albicansP1-Type ATPase in Resistance to Copper and Silver Ion Toxicity

Role of aCandida albicansP1-Type ATPase in Resistance to Copper and Silver Ion Toxicity

Comparison of silver-impregnated with standard multi-lumen central venous catheters in critically ill patients*

Broad-Spectrum Bactericidal Activity of Ag 2 O-Doped Bioactive Glass

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Broad-Spectrum Bactericidal Activity of Ag ₂ O-Doped Bioactive Glass