In vitro efficacy of antibiotic beads in treating abdominal vascular graft infections

Charlton-Ouw, Kristofer M.; Kubrusly, Fernando; Sandhu, Harleen K.; Swick, Michelle C.; Leake, Samuel S.; Gulbis, Brian; Koehler, Theresa M.; Safi, Hazim J.

doi:10.1016/j.jvs.2014.03.241

Cited by 16 publications

(8 citation statements)

References 25 publications

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“…137 Finally, re-infection rates after extra-anatomic bypasses were 0% e 15% 144,175,176 and up to 27% in small series. 137,177 A 30 day mortality rate of 18% (range 11% e 45%) and a late mortality of 32% (range 22% e 44% within 47 months) have been described (Table 11). 137,138,175,177,178 Compromised colonic and pelvic blood supply due to insufficient internal iliac and inferior mesenteric artery perfusion might also lead to complications.…”

Section: Clinical Presentationmentioning

confidence: 99%

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Editor's Choice – European Society for Vascular Surgery (ESVS) 2020 Clinical Practice Guidelines on the Management of Vascular Graft and Endograft Infections

Chakfé

Diener

Lejay

et al. 2020

European Journal of Vascular and Endovascular Surgery

370

322

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DEDICATIONAfter studying medicine at Hanover Medical School and philosophy and social psychology at the Leibniz University Hanover, Omke E. Teebken joined the Christian Albrechts University in Kiel at the end of the 1990s as a research fellow at the Clinic for Cardiovascular Surgery headed by Professor Dr Axel Haverich, whom Omke E. Teebken later followed back to Hanover.In Hanover, besides working as a clinician, Omke E.Teebken was particularly active scientifically, contributing to the establishment of the then newly founded Leibniz Laboratories for Biotechnology and Artificial Organs (LEBAO). His work focused on regenerative medicine and tissue engineering, and subsequently he wrote his habilitation thesis in in this field. After basic training in cardiac surgery, he specialised clinically in vascular surgery and played a pioneering role in the development of this field. Before being appointed director of the Clinic for Vascular Surgery e Endovascular Surgery at the Peine Clinic in 2016, Omke E. Teebken headed the Vascular Surgery e Endovascular Surgery Division of the Department of Cardiothoracic, Thoracic, Transplantation and Vascular Surgery at Hanover Medical School. Professor Teebken was a highly appreciated, committed, and competent colleague and teacher.On 8 April 2019, Professor Teebken passed away after a short and severe illness. He was member and author of the ESVS guideline writing committee, an esteemed colleague, and friend.We will always honor his memory.

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Section: Clinical Presentationmentioning

confidence: 99%

“…For large perigraft abscesses and MRSA infections, EAR and removal of the infected graft may be considered. 17,146,151,176,177,180 7.2.5. Partial or total excision of the material.…”

Section: Clinical Presentationmentioning

confidence: 99%

Editor's Choice – European Society for Vascular Surgery (ESVS) 2020 Clinical Practice Guidelines on the Management of Vascular Graft and Endograft Infections

Chakfé

Diener

Lejay

et al. 2020

European Journal of Vascular and Endovascular Surgery

370

322

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“…Many studies have described antibiotic release from PMMA. An in vitro study by Charlton-Ouw et al 9) reported the efficacy of PMMA when combined with various antibiotics that were frequently used in abdominal graft surgery. However, it could also be a source of infection or form a granulation tissue, provided it remains in the soft tissue; re-operation is essential to remove the beads 4,14,21) .…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Cumulative and Conditional Antibiotic Release from Vancomycin-Embedded Fibrin Sealant and Its Antibacterial Activity : An In Vitro Study

Shin

Sohn

Cho

et al. 2020

J Korean Neurosurg Soc

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Objective : Fibrin sealants have been used for hemostasis, sealant for cerebrospinal fluid leakage, and adhesive barrier in neurosurgery. Further, as its clinical use and role of an effective drug delivery vehicle have been proposed. This study was performed to measure antibacterial activity and continuous local antibiotic release from different concentrations of vancomycin-impregnated fibrin sealant in vitro. Methods : Antibacterial activity was investigated by disk diffusion test by measuring the diameter of the growth inhibition zone of bacteria (methicillin-resistant Staphylococcus aureus, ATCC29213) from vancomycin-embedded fibrin sealant disc diluted at five different concentrations (C1-C5; 8.33, 4.167, 0.83, 0.083, and 0.0083 mg/disc, respectively). Continuous and conditioned release of vancomycin concentration (for 2 weeks and for 5 days, respectively) were also measured using high-performance liquid chromatography (HPLC) method. To mimic the physiologic wound conditions with in vitro, conditioned vancomycin release in phosphate buffer solution (PBS) was measured and replaced PBS for five consecutive days, half a day or completely daily. Results : In the disk diffusion test, the mean diameters of bacterial inhibition zone were 2.54±0.07 cm, 2.61±0.12 cm, and 2.13±0.15 cm (C1, C2, and C3 respectively) but 1.67±0.06 cm and 1.23±0.15 cm in C4 and C5, respectively. Continuous elution test elicited the peak release of vancomycin from the fibrin sealant at 48 hours, with continued release until 2 weeks. However, conditioned vancomycin release decreased to half or more on day 2, however, the sustainable release was measured over the therapeutic dose (10-20 µg/mL) for 5 days and 4 days in assays of half and total exchange of PBS. Conclusion : This study suggests that fibrin sealant can provide an efficient vehicle for antibiotic drug release in a wide range of neurosurgical procedures and the safe and effective therapeutic dose will be at the concentration embedded of 4.167 mg/disc or more of vancomycin.

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“…Reports of successful graft preservation have used various multimodality approaches, incorporating local debridement with some combination of antimicrobial irrigation, [9][10][11] vascularized autologous tissue coverage, 8,12 negative pressure wound therapy, 18 and/or antibiotic-impregnated bead placement. 19,20 Akowuah and colleagues 11 have suggested improved success of graft preservation when a thoracic aortic graft is seeded secondary to an overlying deep sternal wound infection as opposed to the situation of primary graft infection, whereas other groups do not use this as a selection criteria when considering graft preservation. 4,9,10 Whether graft excision versus preservation should be influenced by the presence of deep sternal wound infection as the source for graft seeding, remains unanswered.…”

Section: Discussionmentioning

confidence: 99%

Resection and replacement of thoracic aortic graft infections

Sandhu

Nissen

Mushtaq

et al. 2021

The Journal of Thoracic and Cardiovascular Surgery

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In vitro efficacy of antibiotic beads in treating abdominal vascular graft infections

Cited by 16 publications

References 25 publications

Editor's Choice – European Society for Vascular Surgery (ESVS) 2020 Clinical Practice Guidelines on the Management of Vascular Graft and Endograft Infections

Editor's Choice – European Society for Vascular Surgery (ESVS) 2020 Clinical Practice Guidelines on the Management of Vascular Graft and Endograft Infections

Evaluation of Cumulative and Conditional Antibiotic Release from Vancomycin-Embedded Fibrin Sealant and Its Antibacterial Activity : An In Vitro Study

Resection and replacement of thoracic aortic graft infections

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