In vitro efficacy of chemotherapeutics as determined by 50% inhibitory concentrations in cell cultures of mammary gland tumors obtained from dogs

Simon, Daniella; Knebel, Jan; Baumgärtner, Wolfgang; Aufderheide, Michaela; Meyer‐Lindenberg, Andrea; Nolte, Ingo

doi:10.2460/ajvr.2001.62.1825

Cited by 36 publications

(41 citation statements)

References 36 publications

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“…10,11 Adjuvant chemotherapy is routinely used in women with breast cancer, however, only limited information is available on the efficacy of postoperative chemotherapy in dogs.…”

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confidence: 99%

Postoperative Adjuvant Treatment of Invasive Malignant Mammary Gland Tumors in Dogs with Doxorubicin and Docetaxel

Simón

Schoenrock

Baumgärtner

et al. 2006

Veterinary Internal Medicne

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Background: Treatment outcome after surgery alone is unsatisfactory in dogs with invasive malignant mammary gland tumors.Hypothesis: Adjuvant doxorubicin or docetaxel will improve the treatment outcome in dogs with high-risk malignant mammary gland tumors, and the use of docetaxel will be feasible in affected dogs.Animals: Thirty-one dogs with malignant mammary gland tumors of histologic stages II and III (vascular or lymphatic invasion, regional lymph node metastasis, or distant metastasis) were used.Methods: A prospective clinical trial in which dogs were treated with surgery alone (n 5 19) or also received adjuvant chemotherapy (n 5 12) with doxorubicin or docetaxel was conducted. Docetaxel was given as an IV infusion at a dose of 30 mg/m 2 preceded by dexamethasone and diphenhydramine administration. Results: The recurrence-free interval ranged from 13 to 2,585 days (median not reached); the median metastasis-free interval and overall survival were 294 days and 370 days, respectively. Dogs treated with chemotherapy had a tendency toward higher long-term local control and survival rates, but there was no significant difference in the recurrence-free interval (P 5 .17), time to metastasis (P 5 .71), and overall survival (P 5 .12). Factors found to influence the time to metastasis and overall survival included lymph node metastasis (P 5 .009) and tumor fixation to underlying structures (P 5 .043, time to metastasis), as well as age (P 5 .018) and histologic stage (P , .001, survival). Mild allergic skin reactions were the most frequently observed complications of docetaxel treatment.Conclusions and Clinical Importance: Chemotherapy did not lead to an improved outcome in this population. Docetaxel treatment was well tolerated. Additional investigations of adjuvant chemotherapy in dogs with high-risk mammary cancer are warranted.

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“…10,11 Adjuvant chemotherapy is routinely used in women with breast cancer, however, only limited information is available on the efficacy of postoperative chemotherapy in dogs.…”

mentioning

confidence: 99%

Postoperative Adjuvant Treatment of Invasive Malignant Mammary Gland Tumors in Dogs with Doxorubicin and Docetaxel

Simón

Schoenrock

Baumgärtner

et al. 2006

Veterinary Internal Medicne

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“…The standard protocol for evaluating cell proliferation of adherent tumor cell lines (such as feline ISS) involves a 12-24 hr incubation, removal of the media then addition of the drug in fresh media followed by a proliferation dye. [1] Results demonstrated in this study suggest that the standard protocol may not be a culture requirement for all cancer cell lines.…”

Section: Discussionmentioning

confidence: 81%

“…[1] The standard technique typically consists of obtaining a sample from client-owned pets during surgery, washing, then mincing and dissociating with Trypsin-EDTA or DNAse (200 U=mL) and collagenase (270 U=mL) into a single-cell suspension. The cells are then washed in media and plated in standard tissue culture flasks.…”

Section: Introductionmentioning

confidence: 99%

“…[2,3] Once confluence in the tissue culture flasks has been attained, cells are separated into aliquots for study. [1] A medium containing 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin is typically used for sustaining cell growth, however, lower concentrations of FBS, such as 1%, are routinely used with immortal cell lines to protein-starve cells in order to obtain additional cell analysis. [4] While the addition of serum in media facilitates cell growth, it may mask hormonal and nutritional effects on the cells.…”

Section: Introductionmentioning

confidence: 99%

“…A fluorescent indicator is then added and the cells are evaluated for proliferative capability at different doses of a cytotoxic agent, ultimately establishing IC 50 values. [1,6,7] Assessing cell death and apoptosis is typically performed using flow cytometry and cytologic exam via microscopy. For analysis by flow cytometry, single-cell suspensions with a known number of events, are stained with a dye such as 7-aminoactinomycin-D (7-AAD), which is a DNA intercalating fluorescent dye that differentiates cells based on their viability and stage of cell death.…”

Section: Introductionmentioning

confidence: 99%

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Influence of Different Cell Storage/Culture Conditions on Spontaneous Proliferation and Level of Tyrosine Kinase Receptor Inhibition in Two Feline Injection-Site Sarcoma Cell Lines

Feldhaeusser

Turek

Lawrence

et al. 2013

Journal of Immunoassay and Immunochemistry

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Optimizing cell culture conditions is important when studying cell proliferation and viability, particularly in response to cytotoxic compounds. Altered cell storage conditions can adversely impact proliferation and viability in mortal cell lines. However, little is known regarding the effects on immortal feline cell lines. In the present study, two feline injection-site sarcoma (ISS) cell lines were evaluated under standard culture conditions and three alternative storage/culture conditions for spontaneous proliferation rate and sensitivity to masitinib, a highly selective tyrosine kinase inhibitor with activity against primary and metastatic ISS cell lines. Cell viability was assessed by 7-aminoactinomycin D and cytology. Spontaneous proliferation did not significantly differ across the FBS concentrations (10% vs. 1%) for one cell line, however, with the other cell line spontaneous proliferation was significantly decreased in the 1% FBS 1-step technique, and the cold step technique at both 1% and 10% FBS. When normalized to untreated control cells, the IC50 values for masitinib were comparable across all culture techniques. Furthermore, apoptosis appeared to be the primary mechanism of this proliferation inhibition. Our preliminary findings suggest that select feline sarcoma cell lines cultured in 10% FBS yield comparable cytotoxicity data even when subjected to varying storage/culture conditions.

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Multimodal Therapy

BVSc¹,

Séguin²

2012

Veterinary Surgical Oncology

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In vitro efficacy of chemotherapeutics as determined by 50% inhibitory concentrations in cell cultures of mammary gland tumors obtained from dogs

Cited by 36 publications

References 36 publications

Postoperative Adjuvant Treatment of Invasive Malignant Mammary Gland Tumors in Dogs with Doxorubicin and Docetaxel

Postoperative Adjuvant Treatment of Invasive Malignant Mammary Gland Tumors in Dogs with Doxorubicin and Docetaxel

Influence of Different Cell Storage/Culture Conditions on Spontaneous Proliferation and Level of Tyrosine Kinase Receptor Inhibition in Two Feline Injection-Site Sarcoma Cell Lines

Multimodal Therapy

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