In vitro efficacy of gentamicin released from collagen sponge in eradication of bacterial biofilm preformed on hydroxyapatite surface

Mączyńska, Beata; Secewicz, Anna; Smutnicka, Danuta; Szymczyk, Patrycja; Dudek-Wicher, Ruth; Junka, Adam; Bartoszewicz, Marzenna

doi:10.1371/journal.pone.0217769

Cited by 22 publications

(19 citation statements)

References 42 publications

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“…Moreover, the concentrations of antibiotic able to eradicate microbial biofilms are relatively high. Maczynska et al [ 35 ] indicated that high local concentrations of gentamicin released from collagen sponge eradicated the biofilm formed not only by gentamicin-sensitive strains but, to some extent, also by these that display a resistance pattern in routine diagnostics. Nevertheless, values of effective concentrations of gentamycin were in aforementioned Maczynska’s work above 100 mg/L in case of P. aeruginosa and S. aureus biofilm and above 700 mg/L in case of Klebsiella pneumoniae .…”

Section: Discussionmentioning

confidence: 99%

In Vitro Evaluation of Polihexanide, Octenidine and NaClO/HClO-Based Antiseptics against Biofilm Formed by Wound Pathogens

Krasowski¹,

Junka

Paleczny

et al. 2021

Membranes

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Chronic wounds complicated with biofilm formed by pathogens remain one of the most significant challenges of contemporary medicine. The application of topical antiseptic solutions against wound biofilm has been gaining increasing interest among clinical practitioners and scientific researchers. This paper compares the activity of polyhexanide-, octenidine- and hypochlorite/hypochlorous acid-based antiseptics against biofilm formed by clinical strains of Candida albicans, Staphylococcus aureus and Pseudomonas aeruginosa. The analyses included both standard techniques utilizing polystyrene plates and self-designed biocellulose-based models in which a biofilm formed by pathogens was formed on an elastic, fibrinous surface covered with a fibroblast layer. The obtained results show high antibiofilm activity of polihexanide- and octenidine-based antiseptics and lack or weak antibiofilm activity of hypochlorite-based antiseptic of total chlorine content equal to 80 parts per million. The data presented in this paper indicate that polihexanide- or octenidine-based antiseptics are highly useful in the treatment of biofilm, while hypochlorite-based antiseptics with low chlorine content may be applied for wound rinsing but not when specific antibiofilm activity is required.

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Section: Discussionmentioning

confidence: 99%

In Vitro Evaluation of Polihexanide, Octenidine and NaClO/HClO-Based Antiseptics against Biofilm Formed by Wound Pathogens

Krasowski¹,

Junka

Paleczny

et al. 2021

Membranes

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“…We are aware of the preliminary character and limitations of our in vitro study and we are fully conscious of the fact that our data represents first (but necessary to take) step in the research algorithm starting from in vitro results, going through the animal model study and ending in trials on patients. We hypothesize that, in the future, selected components (active substances) of EOs may be chemisorbed within organic, non-cytotoxic carrier and applied for the local treatment of osteomyelitis, as it is presently performed in the case of gentamycin-saturated collagen sponge [ 73 ]. The specific EOs’ volatile fractions may be also applied, to reach biofilm hidden within wound pockets.…”

Section: Discussionmentioning

confidence: 99%

Activity of Liquid and Volatile Fractions of Essential Oils against Biofilm Formed by Selected Reference Strains on Polystyrene and Hydroxyapatite Surfaces

et al. 2021

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Biofilms are surface-attached, structured microbial communities displaying higher tolerance to antimicrobial agents in comparison to planktonic cells. An estimated 80% of all infections are thought to be biofilm-related. The drying pipeline of new antibiotics efficient against biofilm-forming pathogens urges the search for alternative routes of treatment. Essential Oils (EOs), extracted from medicinally important plants, are a reservoir of bioactive compounds that may serve as a foothold in investigating novel antibiofilm compounds. The aim of this study was to compare antimicrobial activity of liquid and volatile fractions of tested EOs against biofilm-forming pathogens using different techniques. In this research, we tested five EOs, extracted from Syzygium aromaticum L., Boswelia serrata Roxb., Juniperus virginiana L., Pelargonium graveolens L. and Melaleuca alternifolia Cheel., against planktonic and biofilm forms of five selected reference strains, namely Staphylococcus aureus, Enterococcus faecalis, Klebsiella pneumoniae, Pseudomonas aeruginosa, Escherichia coli, and Candida albicans. To obtain cohesive results, we applied four various methodological approaches: to assess the activity of the liquid fraction of EOs, disc diffusion and the microdilution method were applied; to test EOs’ volatile fraction, the AntiBioVol assay and modified Antibiofilm Dressing Activity Measurement (A.D.A.M.) were used. The molecular composition and dynamics of antimicrobial substances released from specific EOs was measured using Gas Chromatography–Mass Spectrometry (GC-MS). The antimicrobial potency of EO’s volatile fraction against biofilm formed by tested strains differed from that of the liquid fraction and was related to the molecular weight of volatile compounds. The liquid fraction of CW-EO and volatile fraction of F-EO acted in the strongest manner against biofilm of C. albicans. The addition of 0.5% Tween 20 to liquid phase, enhanced activity of G-EO against E. coli and K. pneumoniae biofilm. EO activity depended on the microbial species it was applied against and the chosen assessment methodology. While all tested EOs have shown a certain level of antimicrobial and antibiofilm effect, our results indicate that the choice of EO to be applied against a specific biofilm-forming pathogen requires careful consideration with regard to the above-listed aspects. Nevertheless, the results presented in this research contribute to the growing body of evidence indicating the beneficial effects of EOs, which may be applied to fight biofilm-forming pathogens.

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“…Sponges for local and rapid treatment of osteomyelitis appears to be a growing area of interest, particularly in military applications, as they are readily available to pack into traumatic wounds and rapidly deliver therapeutics directly to the site of infection. 29 Reported materials of sponges include chitosan, chitosan-polycaprolactone blends, and collagen, which have been used to deliver gentamicin, 116,152 ciprofloxacin, 29,153 amikacin, 154,155 vancomycin, [154][155][156] rifampin, 29 and others. 157 In two comparative studies in humans, gentamicin loaded collagen sponges matched the efficacy of traditionally used PMMA beads in osteomyelitis treatment, while reducing the need for secondary surgical procedures.…”

Section: Spongesmentioning

confidence: 99%

Therapeutics and delivery vehicles for local treatment of osteomyelitis

Cobb

McCabe

Priddy

2020

Journal Orthopaedic Research

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Osteomyelitis, or the infection of the bone, presents a major complication in orthopedics and may lead to prolonged hospital visits, implant failure, and in more extreme cases, amputation of affected limbs. Typical treatment for this disease involves surgical debridement followed by long‐term, systemic antibiotic administration, which contributes to the development of antibiotic‐resistant bacteria and has limited ability to eradicate challenging biofilm‐forming pathogens including Staphylococcus aureus—the most common cause of osteomyelitis. Local delivery of high doses of antibiotics via traditional bone cement can reduce systemic side effects of an antibiotic. Nonetheless, growing concerns over burst release (then subtherapeutic dose) of antibiotics, along with microbial colonization of the nondegradable cement biomaterial, further exacerbate antibiotic resistance and highlight the need to engineer alternative antimicrobial therapeutics and local delivery vehicles with increased efficacy against, in particular, biofilm‐forming, antibiotic‐resistant bacteria. Furthermore, limited guidance exists regarding both standardized formulation protocols and validated assays to predict efficacy of a therapeutic against multiple strains of bacteria. Ideally, antimicrobial strategies would be highly specific while exhibiting a broad spectrum of bactericidal activity. With a focus on S. aureus infection, this review addresses the efficacy of novel therapeutics and local delivery vehicles, as alternatives to the traditional antibiotic regimens. The aim of this review is to discuss these components with regards to long bone osteomyelitis and to encourage positive directions for future research efforts.

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In vitro efficacy of gentamicin released from collagen sponge in eradication of bacterial biofilm preformed on hydroxyapatite surface

Cited by 22 publications

References 42 publications

In Vitro Evaluation of Polihexanide, Octenidine and NaClO/HClO-Based Antiseptics against Biofilm Formed by Wound Pathogens

In Vitro Evaluation of Polihexanide, Octenidine and NaClO/HClO-Based Antiseptics against Biofilm Formed by Wound Pathogens

Activity of Liquid and Volatile Fractions of Essential Oils against Biofilm Formed by Selected Reference Strains on Polystyrene and Hydroxyapatite Surfaces

Therapeutics and delivery vehicles for local treatment of osteomyelitis

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