In vitro embryo germination and proliferation of pistachio (Pistacia vera L.)

Benmahioul, Benamar; Kaïd-Harche, Meriem; Dorion, N.; Daguin, F.

doi:10.1016/j.scienta.2009.05.029

Cited by 28 publications

(29 citation statements)

References 24 publications

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“…Verificou-se redução do comprimento dos brotos, com o aumento das concentrações de BAP, obtendo-se o maior comprimento (67,93 mm) em meio isento de regulador de crescimento vegetal (Figura 3). Esse resultado é corroborado por Benmahioul et al (2009), que constataram melhor crescimento (26 e 25 mm) da parte aérea de embriões isolados de pistache (Pistacia vera L.), em meios sólidos ricos em nutrientes (DKW e MS) na ausência de regulador de crescimento vegetal.…”

Section: Resultsunclassified

“…Alguns padrões morfogênicos são frequentemente relatados, como: a neoformação de raízes e gemas caulinares, em murmuru -Astrocaryum ule (Pereira et al, 2006), Annona glabra L. (Santana et al, 2008), pistachio -Pistacia vera L. (Benmahioul et al, 2009); a formação de calos e embriões somáticos em canafístula-Peltophorum dubium (Bassan et al, 2006), Heliconia bihai (L.) L. cv. Lobster Claw Two (Ulisses et al, 2011) e em maracujá -Passiflora edulis Sims (Pinto et al, 2012), estando intimamente ligados aos fatores supracitados, bem como às condições de cultivo e ao tipo de explante utilizado.…”

Section: Introductionunclassified

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Resposta morfogênica de embriões zigóticos de Erythrina velutina Willd: (Leguminosae) cultivados in vitro

et al. 2014

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Resposta morfogênica de embriões zigóticos de Erythrina velutinaWilld. (Leguminosae) cultivados in vitro O cultivo in vitro de embriões zigóticos é uma técnica promissora para se avançar no estudo do desenvolvimento embrionário e da quebra da dormência de sementes. Diante do exposto, objetivou-se, com este trabalho, avaliar o efeito dos reguladores vegetais 6-benzilaminopurina (BAP) e ácido naftalenoacético (ANA) no potencial morfogenético, in vitro, de embriões zigóticos de mulungu. Embriões zigóticos maduros, oriundos de sementes foram utilizados inteiros, ou seccionados em plúmula, região intermediária e radícula, sendo posteriormente inoculados em meio de cultura WPM, suplementado com combinações de BAP (0,0; 2,0; 4,0; 8,0; 12,0 e 16,0 µM) e ANA (0,0; 1,0 e 2,0 µM), acrescido de 87,64 mM de sacarose e solidificado com 0,7% de ágar. Após 30 dias, avaliaram-se a percentagem de regeneração dos embriões e ápice plumular, o número de brotos, o número de folhas, o comprimento da parte aérea dos brotos, o número de raízes e a percentagem de formação de calos oriundos da região intermediária e da radícula. É possível a regeneração in vitro de mulungu, a partir dos explantes plúmula e embriões zigóticos inteiros, cultivados em meio de cultura WPM, suplementado com 4,0 µM de BAP. Regiões intermediárias e da radícula promoveram a formação de calos compactos (96,06%), na combinação de 10,63 µM BAP e 2,0 µM de ANA. Palavras Morphogenic response of zygotic embryos of Erythrina velutina Willd. (Leguminosae) cultivated in vitroThe in vitro culture of zygotic embryos represents a promising technique to study the embryonic development and dormancy in seeds. The objective of this study was to determine the effect of plant growth regulators, 6-benzylaminopurine (BAP) and naphthalene acetic (NAA), in the morphogenetic potential of in vitro zygotic embryos of mulungu. Mature zygotic embryos from seeds were either used as a whole or sectioned in plumule, radicle and intermediate region, and subsequently inoculated in WPM medium supplemented with combinations of BAP (0.0, 2.0, 4, 0, 8.0, 12.0 and 16.0 µM) and NAA (0.0, 1.0 and 2.0 µM), added with 87.65 mM sucrose and 0.7% agar. After 30 days, the following parameters were evaluated: regeneration percentage of embryos and plumularapice, number

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Section: Resultsunclassified

Section: Introductionunclassified

Resposta morfogênica de embriões zigóticos de Erythrina velutina Willd: (Leguminosae) cultivados in vitro

et al. 2014

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“…Sucrose added to the medium also has a strong effect on seed germination, since its requirement depends on the embryonic stage of development, being used as an energy source and/or to maintain an adequate osmotic equilibrium (Benmahioul et al, 2009;Li et al, 2012;Manzur;Penella;Rodriguez-Burruezo, 2013). Therefore, it is possible to state that the germination of G. americana seeds has low requirements with respect to the mineral nutrients added to the medium, and the addition of sucrose is essential for the maintenance of osmotic balance.…”

Section: Different Compositions In the Culture Mediummentioning

confidence: 99%

Optimization of jenipapo in vitro seed germination process

et al. 2016

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The in vitro seed germination is an effective alternative for quickly obtaining explants with sanitary quality. However, jenipapo seeds present slow and uneven germination. Therefore, internal and external factors to seed which directly interfere in the process, they must be identified, in order to adapt better techniques to obtain seedlings. In this sense, this work aimed to optimize the in vitro germination of Genipa americana L. seeds by evaluating different factors (light quality, GA 3 treatment, pre-soaking in distilled water, growing media and stratification in the dark). It was found that the seed germination of G. americana was indifferent to light, however, the best results were obtained under conditions of continuous darkness; There was no effect of the application of exogenous GA 3 ; The pre-soaking in distilled water for 48 h contributes to obtaining better germination rates; And the reduction in MS medium salts, and laminating the pretreatment in the dark maximizes the germination potential of seeds.Therefore, the optimal conditions for in vitro germination of G. americana L. seeds requires pre-soaking in distilled water for 48 hours and inoculation into culture media consisting of ½ MS + 15 g L -1 sucrose, with stratification in the dark for 16 days, followed by the transfer to growth chambers with lighting provided by white fluorescent lamps.Index terms: Genipa americana L.; in vitro culture; ligth quality; Rubiaceae; stratification in the dark. RESUMOA germinação in vitro de sementes é uma alternativa eficaz para obtenção de explantes com rapidez e qualidade sanitária. No entanto, sementes de jenipapeiro apresentam germinação lenta e desuniforme. Para tanto, fatores internos e externos às sementes que interferem diretamente no processo, devem ser identificados, visando à adaptação de melhores técnicas para obtenção de mudas. Neste sentido, objetivou-se otimizar a germinação in vitro de sementes de Genipa americana L. por meio da avaliação de diferentes fatores (qualidade de luz, tratamento com GA 3 , pré-embebição em água destilada, diferentes composições no meio de cultivo e estratificação no escuro). Verificou-se que a germinação das sementes foi indiferente a luz, no entanto, os melhores resultadados foram obtidos sob condições de escuro contínuo; Não houve efeito da aplicação de GA 3 exógeno; A pré-embebição em água destilada durante 48h contribui para obtenção de melhores taxas de germinação; E a redução de sais do meio MS, bem como o pré-tratamento de estratificação no escuro maximizam o potencial germinativo das sementes. Portanto, as condições ótimas para germinação in vitro de sementes de G. americana L. requer a pré-embebição em água destilada por 48 horas e a inoculação em meio de cultivo constituído de ½ MS + 15 g L-1 de sacarose, com estratificação no escuro por 16 dias, seguida pela transferência a salas de crescimento com iluminação fornecida por lâmpadas fluorescentes branca.Termos para indexação: Genipa americana L.; cultivo in vitro; qualidade de luz; Rubiaceae; estratif...

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“…The seeds were then rinsed three times with sterile distilled water. To facilitate the extraction of embryonic axes, seeds were kept overnight in sterile distilled water (Benmahioul et al 2009). …”

Section: Plant Materials and Methods Of Disinfectionmentioning

confidence: 99%

“…The isolated embryonic axes were seeded in glass test tubes (22 × 150 mm) containing 15 ml of MS0, a Murashige and Skoog (1962) medium without growth regulators and supplemented with 2 g·l -1 of activated charcoal and 4 g·l -1 agar (Benmahioul et al 2009). Cultures were placed at 22°C, in the dark for the first three days, and then kept at a 16 h photoperiod with irradiance of 40 μmol·m -2 ·s -1 .…”

Section: Plant Materials and Methods Of Disinfectionmentioning

confidence: 99%

Cryopreservation of Pistacia vera embryonic axes

Benmahioul¹,

Daguin²,

Kaïd-Harche³

2015

J. For. Sci.

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This preliminary study investigated the conservation of Pistacia vera genetic resources using seeds and isolated embryonic axes. First, the effect of storing seeds in ambient conditions on embryo viability was evaluated by in vitro culture. The germination rate of P. vera embryonic axes gradually decreased from 100% to 31% after 30-month storage of seeds. Cryopreservation may thus be necessary for the long-term conservation of embryos. A simple protocol was set up using embryonic axes. It included a single dehydration step with silica gel prior to direct freezing in liquid nitrogen (-196°C). The optimal germination rate was obtained after 60 min dehydration (water content of 0.2 grams of water per gram of dry weight [g·g -1 DW]). However, 90 minutes of dehydration (0.14 g·g -1 DW) were necessary to obtain seedlings whose qualitative development was equivalent to that of the control embryonic axes.Keywords: seed; dehydration; water content; liquid nitrogen; germinationPistachio is a dicotyledonous angiosperm belonging to the family Anacardiaceae. According to Zohary (1952), the genus Pistacia comprises four sections and 11 species. Pistacia vera L. is the only species producing edible seeds (Joley 1979). With its seeds and its high fodder value, the pistachio is a vital source of income for farmers. However, different types of problems can affect the cultivation of this species including orchard ageing, damage due to pests and diseases. The conservation of Pistacia genetic resources is consequently essential.Seed banks are the most common means of preserving plant genetic resources, at least for species which produce orthodox seeds (i.e. seeds that can withstand dehydration, thus ensuring their long-term viability). For example, pea (Pisum sativum) seeds were shown to be viable after more than 23 years and maize (Zea mays) seeds after 19 years, even though they were stored in ambient conditions (Nagel, Borner 2010). A few 200-years-old seeds of species originating from the Cape floristic region in South Africa were even able to germinate (Daws et al. 2007). In contrast, seeds of other species, called recalcitrant, cannot survive dehydration or storage at low temperatures and consequently cannot be stored for a long period. Most recalcitrant seeds are produced by woody species, shrubs or trees (Li, Pritchard 2009). The influence of conservation on the viability of seeds of P. vera L. is almost unknown. This is a limiting factor for the optimal management of seed stocks by seed producers and for the long-term conservation of P. vera genetic resources. According to Jacquy (1972), pistachio seeds lose 50% of their viability after one year of storage. Plant tissue culture has been used for P. vera L. for ex situ conservation (Ozden-Tokatli et al. 2010). The use of in vitro culture for the conservation of species with recalcitrant seeds has many advantages, including maintenance under aseptic conditions. However, when used for long periods, it has many disadvantages including the risk of genetic variation and...

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In vitro embryo germination and proliferation of pistachio (Pistacia vera L.)

Cited by 28 publications

References 24 publications

Resposta morfogênica de embriões zigóticos de Erythrina velutina Willd: (Leguminosae) cultivados in vitro

Resposta morfogênica de embriões zigóticos de Erythrina velutina Willd: (Leguminosae) cultivados in vitro

Optimization of jenipapo in vitro seed germination process

Cryopreservation of Pistacia vera embryonic axes

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