In vitro enzyme inhibition, antibacterial, UHPLC-MS chemical profiling and in silico studies of Indigofera argentea Burm. f. for potential biopharmaceutical application

Arshad, Adeel; Rehman, Tayyeba; Saleem, Hammad; Khan, Shakeel Ahmad; Saleem, Muhammad; Tousif, Muhammad Imran; Ahemad, Saeed; Ahemad, Nafees; Abdallah, Hassan H.; Mahomoodally, Fawzi M.

doi:10.1016/j.sajb.2020.12.001

Cited by 4 publications

(3 citation statements)

References 31 publications

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“…The chlorogenic acid and p-coumaric acid were also described in a earlier study on the ethanolic extract of this plant (Javed et al, 2020), however, their cumulative quantity was greater in the methanolic extract as observed in our study. The I. argentea was also containing many phenolic and other bioactive phytochemicals as reported by another study (Arshad et al, 2021). So, the IaMe extract can be considered as a source of bioactive phytochemicals, especially the polyphenols.…”

Section: Discussionmentioning

confidence: 67%

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In vitro and split-faced placebo-controlled in vivo study on the skin rejuvenating effects of cream loaded with bioactive extract of Indigofera argentea Burm.f

Arshad,

Shoaib Khan,

Khan

et al. 2024

Front. Pharmacol.

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The bioactive extracts of traditional medicinal plants are rich in polyphenols and help to rejuvenate skin. The study was designed to assess the skin rejuvenating effects of a stable cream enriched with 4% I. argentea (IaMe) extract. The quantity of polyphenols by spectrophotometric methods was TPC, 101.55 ± 0.03 mg GAE/g and total flavonoid content; 77.14 ± 0.13 mg QE/g, while HPLC-PDA revealed gallic acid; 4.91, chlorogenic acid 48.12, p-coumaric acid 0.43, and rutin 14.23 μg/g. The significant results of biological activities were observed as DPPH; 81.81% ± 0.05%, tyrosinase; 72% ± 0.23% compared to ascorbic acid (92.43% ± 0.03%), and kojic acid (78.80% ± 0.19%) respectively. Moreover, the promising sun protection effects Sun protection factor of extract (20.53) and formulation (10.59) were observed. The active cream formulation (w/o emulsion) was developed with liquid paraffin, beeswax, IaMe extract, and ABIL EM 90, which was stable for 90 days as shown by various stability parameters. The rheological results demonstrated the active formulation’s non-Newtonian and pseudo-plastic characteristics and nearly spherical globules by SEM. The IaMe loaded cream was further investigated on human trial subjects for skin rejuvenating effects and visualized in 3D skin images. Herein, the results were significant compared to placebo. IaMe formulation causes a substantial drop in skin melanin from −1.70% (2 weeks) to −10.8% (12 weeks). Furthermore, it showed a significant increase in skin moisture and elasticity index from 7.7% to 39.15% and 2%–30%, respectively. According to the findings, Indigofera argentea extract has promising bioactivities and skin rejuvenating properties, rationalizing the traditional use and encouraging its exploitation for effective and economical cosmeceuticals.

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Section: Discussionmentioning

confidence: 67%

“…This discovery emphasises how important botanical extract is to the cream's occlusive qualities. Prior research has highlighted the function of components derived from plants, as those in I. argentea, in creating efficient occlusive walls (Arshad et al, 2021;Khan K.-u.-R. et al, 2023).…”

Section: Discussionmentioning

confidence: 99%

In vitro and split-faced placebo-controlled in vivo study on the skin rejuvenating effects of cream loaded with bioactive extract of Indigofera argentea Burm.f

Arshad,

Shoaib Khan,

Khan

et al. 2024

Front. Pharmacol.

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“…Bryophyllum pinnatum (Ahmed et al, 2017;Ahmad et al, 2019;Arshad et al, 2020;Latif et al, 2020). In seven medicinal plant species included; Amberboa ramose, Barleria acanthoides, Isatis costata, Potentilla evestita, Ranunculus muricatus, Ranunculus repens, and Syzygium aromaticum, several phytochemicals were isolated and elucidated for potential XOI activity (Khan et al, 2008;Karim et al, 2009;Saeed and Shahwar, 2015;Rauf et al, 2016;Raziq et al, 2017).…”

Section: Figure 3 Parts Of Medicinal Plants Used In Different Studiesmentioning

confidence: 99%

Medicinal plants of Pakistan and their xanthine oxidase inhibition activity to treat gout: a systematic review

ASHIQ,

HUSSAIN,

ISLAM

et al. 2021

Turk J Bot

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Green synthesis, characterization, and in vitro and in vivo biological screening of iron oxide nanoparticles (Fe₃O₄) generated with hydroalcoholic extract of aerial parts of Euphorbia milii

Rauf,

Ahmad,

Zhang

et al. 2024

Green Processing and Synthesis

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In this work, iron oxide nanoparticles (IONPs) were synthesized using green methods. The structural morphological and optical properties of nanoparticles (NPs) were investigated by scanning electron microscopy (SEM), X-ray diffraction (XRD), ultraviolet–visible spectroscopy, and Fourier transform infrared spectroscopy. Based on the SEM analysis, spherical NPs with a size distribution in the range of 50–70 nm were simulated with slight variations in shapes, and the corresponding XRD was calculated. The calculated XRD patterns were then averaged. Well-defined crystalline structure of a single-phase spinel structure and a spherical shape of IONPs were observed. The in vitro and in vivo biological activities of IONPs synthesized with the hydroalcoholic extract of Euphorbia milii’s aerial parts were studied. The antibacterial effects were evaluated against Staphylococcus aureus, and the enzyme inhibitory effects of the IONPs and E. milii extract on various enzymes were assessed. The IONPs demonstrated significant inhibitory activity against urease (89.09% inhibition), α-glucosidase (72.87% inhibition), carbonic anhydrase II (87.09% inhibition), and xanthine oxidase (85.09% inhibition). The E. milii extract also exhibited considerable inhibition of these enzymes. The IC50 values for urease, α-glucosidase, carbonic anhydrase II, and xanthine oxidase inhibition by IONPs were 26.09, 59.09, 0.18, and 7.71 µg·mL−1, respectively. The anticancer activity of the IONPs and crude extract was also evaluated. The minimum IC50 values were recorded against MDR 2780AD, i.e., 0.85 (extract) and 0.54 (IONPs). Both of the tested samples were found to be significant (p < 0.001) analgesic and anti-inflammatory. In contrast, the IONPs were found sedative at all tested doses, and the extract showed a significant (p < 0.01) sedative effect at higher doses only.

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In vitro enzyme inhibition, antibacterial, UHPLC-MS chemical profiling and in silico studies of Indigofera argentea Burm. f. for potential biopharmaceutical application

Cited by 4 publications

References 31 publications

In vitro and split-faced placebo-controlled in vivo study on the skin rejuvenating effects of cream loaded with bioactive extract of Indigofera argentea Burm.f

In vitro and split-faced placebo-controlled in vivo study on the skin rejuvenating effects of cream loaded with bioactive extract of Indigofera argentea Burm.f

Medicinal plants of Pakistan and their xanthine oxidase inhibition activity to treat gout: a systematic review

Green synthesis, characterization, and in vitro and in vivo biological screening of iron oxide nanoparticles (Fe₃O₄) generated with hydroalcoholic extract of aerial parts of Euphorbia milii

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In vitro enzyme inhibition, antibacterial, UHPLC-MS chemical profiling and in silico studies of Indigofera argentea Burm. f. for potential biopharmaceutical application

Cited by 4 publications

References 31 publications

In vitro and split-faced placebo-controlled in vivo study on the skin rejuvenating effects of cream loaded with bioactive extract of Indigofera argentea Burm.f

In vitro and split-faced placebo-controlled in vivo study on the skin rejuvenating effects of cream loaded with bioactive extract of Indigofera argentea Burm.f

Medicinal plants of Pakistan and their xanthine oxidase inhibition activity to treat gout: a systematic review

Green synthesis, characterization, and in vitro and in vivo biological screening of iron oxide nanoparticles (Fe3O4) generated with hydroalcoholic extract of aerial parts of Euphorbia milii

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Green synthesis, characterization, and in vitro and in vivo biological screening of iron oxide nanoparticles (Fe₃O₄) generated with hydroalcoholic extract of aerial parts of Euphorbia milii