In vitro estrogenic activities of Chinese medicinal plants traditionally used for the management of menopausal symptoms

Zhang, C.Z.; Wang, Yafei; Zhang, Y.; Chen, J.P.; Liang, Xinmiao

doi:10.1016/j.jep.2005.01.033

Cited by 123 publications

(64 citation statements)

References 23 publications

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“…The flavonoid concentration in Polygonum cuspidatum was 62.3 ± 6 mg/g, lower than Polygonum aviculare L. (112.7 ± 13 mg/g), but higher than propolis (23). The higher content of total phenolics and flavonoids in Polygonum cuspidatum have been correlated with hepatitis B virus inhibition (13), bacterial DNA primase inhibition (15), acyl-coenzyme A acyltransferase activity (16), inhibition of copper catalyzed oxidation for low-density lipoprotein (32), inhibition of platelet clotting and arachidonate metabolism, liver injury from peroxidized oil (33), having cancer-chemopreventive activities (34), and estrogenic activities (14,35). Taken together, this study indicates that Polygonum cuspidatum extract clearly has antioxidant effects.…”

Section: Resultsmentioning

confidence: 99%

Antioxidant activity of extract from Polygonum cuspidatum

Hsu¹,

Chan²,

Chang³

2007

Biol. Res.

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Numerous diseases are induced by free radicals via lipid peroxidation, protein peroxidation and DNA damage. It has been known that a variety of plant extracts have antioxidant activities to scavenge free radicals. Whether Polygonum cuspidatum Sieb. et Zucc has antioxidant activity is unknown. In this study, dried roots of Polygonum cuspidatum were extracted by ethanol and the extract was lyophilized. Free radical scavenging assays, superoxide radical scavenging assays, lipid peroxidation assays and hydroxyl radical-induced DNA strand scission assays were employed to study antioxidant activities. The results indicate that the IC50 value of Polygonum cuspidatum extract is 110 μg/ml in free radical scavenging assays, 3.2 μg/ml in superoxide radical scavenging assays, and 8 μg/ml in lipid peroxidation assays, respectively. Furthermore, Polygonum cuspidatum extract has DNA protective effect in hydroxyl radical-induced DNA strand scission assays. The total phenolics and flavonoid content of extract is 641.1 ± 42.6 mg/g and 62.3 ± 6.0 mg/g. The results indicate that Polygonum cuspidatum extract clearly has antioxidant effects.

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Section: Resultsmentioning

confidence: 99%

Antioxidant activity of extract from Polygonum cuspidatum

Hsu¹,

Chan²,

Chang³

2007

Biol. Res.

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“…Bunge, is a herb used in traditional Chinese medicine. Previous studies have reported that Radix Astragali extracts showed estrogenic activity in vitro (Zhang et al, 2005) and prevented bone loss in ovariectomized (OVX) rats (Kim et al, 2003). Recently, it has been shown that the combination of Rubus coreanus Miquel and A. membranaceus Bunge extracts prevented tibial bone loss and improved bone biomechanical properties in OVX-induced osteoporosis in mice (Jung Koo et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Effect of Astragalus polysaccharides on ovariectomy-induced osteoporosis in mice

Huo¹,

Sun²

2016

Genet. Mol. Res.

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ABSTRACT. Postmenopausal osteoporosis, a common type of osteoporosis in women, has become a serious public health issue. Astragalus polysaccharides (APS), possessing various pharmacological activities, are the active ingredients of Radix Astragali. It can be advantageous in the treatment of postmenopausal osteoporosis. In the present study, we evaluated the potential therapeutic effects of APS on postmenopausal osteoporosis by using a mice model induced by ovariectomy (OVX). Forty-eight female 6-week-old outbred ICR mice were randomly divided into six groups (N = 8): Sham group, OVX group, 17 b-estradiol (E2, 0.1 mg/kg)-treated OVX group, and APS (at three doses: 100, 200, and 400 mg/kg)-treated OVX groups. The effect of APS on the bone mineral density (BMD) was determined using dualenergy X-ray absorptiometry. The serum levels of receptor activator of nuclear factor kappa-B ligand (RANKL), osteoprotegerin (OPG), osteocalcin, and tumor necrosis factor (TNF)-a were measured using ELISA kits. The results revealed that APS exerted significant antiosteoporotic activity by increasing the BMD considerably in a dosedependent manner. APS treatment reduced the serum RANKL levels considerably and increased the serum OPG levels, thereby lowering the ratio of RANKL/OPG. Furthermore, APS also markedly reduced osteocalcin and TNF-a concentration in OVX-induced postmenopausal osteoporosis mice model. These results showed that APS exerts a protective effect on bone loss in OVX mice. The molecular mechanism underlying this effect be the reduction of bone resorption and inhibition of osteoclastogenesis. Our findings suggest that APS may be a potential strategy for the prevention and treatment of postmenopausal osteoporosis.

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“…Recombinant yeast (Saccharomyces cerevisae strain BJ3505, originally developed by Glaxo and kindly donated by Institute of Hydrobiology, Chinese Academy of Sciences) was used to assess the oestrogenic activity of fractions obtained from P. cuspidatum. The method used was that of Zhang et al (2005). 17β-estradiol (E2) was used as a positive control and dimethyl sulphoxide (DMSO) as a negative (vehicle) control for oestrogenic activity.…”

Section: Methodsmentioning

confidence: 99%