In vitro estrogenic activity of Achillea millefolium L.

Innocenti, Gabbriella; Vegeto, Elisabetta; Dall’Acqua, Stefano; Ciana, Paolo; Giorgetti, Michela; Agradi, E.; Sozzi, Andrea; Fico, Gelsomina; Tomé, Franca

doi:10.1016/j.phymed.2006.05.005

Cited by 85 publications

(58 citation statements)

References 19 publications

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“…Regarding the composition of the flavonoid complex, the patterns of distribution in the analyzed samples of yarrow flowers are characterized by the dominance of apigenin-7-O-glucoside (3.334-7.546 mg/g) and luteolin-7-O-glucoside (2.107-5.885 mg/g), as well as their corresponding free aglycones -apigenin (0.448-2.562 mg/g) and luteolin (0.561-1.253 mg/g). Rather high amounts of apigenin and luteolin are of high importance in the pharmacological profile of yarrow, particularly in spasmolytic and estrogenic effects (6,13). Concerning the pattern of flavonoid distribution, the results in present study are in good agreement with previously published data on A. millefolium (19,20).…”

Section: Resultssupporting

confidence: 91%

“…The first cluster (I) bringing together 10 populations (No. 13,17,18,12,9,14,15,16,4, and 10) is characterized by lower than the mean total content of the identified phenolics (13.290-20.846 mg/g). Cluster I united two lower level clusters (I-1 and I-2).…”

Section: Resultsmentioning

confidence: 91%

“…Two flavonoids casticin (11) and centaureidin (12), respectively, derived from A. millefolium and A. clavennae L., were Medicina (Kaunas) 2008; 44 (10) EKSPERIMENTINIAI TYRIMAI observed to exert cytotoxic activity. The results from in vitro assay, based on recombinant MCF-7 cells, indicated that flavonoids from A. millefolium exhibit estrogenic activity (13). Moreover, recent experiments have confirmed that phenolic substances are completely extracted into the common application forms of yarrow; thereby, these compounds may exert their polyvalent pharmacological effects (6,7).…”

Section: Introductionmentioning

confidence: 99%

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Variability of phenolic compounds in flowers of Achillea millefolium wild populations in Lithuania

Benetis¹,

Radušienė²,

Janulis³

2008

Medicina

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Section: Resultssupporting

confidence: 91%

Section: Resultsmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 99%

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Variability of phenolic compounds in flowers of Achillea millefolium wild populations in Lithuania

Benetis¹,

Radušienė²,

Janulis³

2008

Medicina

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“…This method has been adapted for evaluation of the Thai P. mirifica population and in capable of ranking plants based on their estrogenic activity in relation to vaginal epithelium differentiation [18]. These findings confirmed that the Thai P. mirifica population could be used as an alternative to estrogen.The MCF-7 proliferation assay which is based on proliferation of ERα positive human mammary adenocarcinoma cells, is a well accepted method for evaluation of estrogenic effects initiated by phytochemicals [19], animal products [20] and plants [21]. This system has also been used in evaluating the anti-estrogenic activity of chemicals [22].…”

mentioning

confidence: 99%

“…The MCF-7 proliferation assay which is based on proliferation of ERα positive human mammary adenocarcinoma cells, is a well accepted method for evaluation of estrogenic effects initiated by phytochemicals [19], animal products [20] and plants [21]. This system has also been used in evaluating the anti-estrogenic activity of chemicals [22].…”

mentioning

confidence: 99%

Determination of the Estrogenic Activity of Wild Phytoestrogen-rich Pueraria mirifica by MCF-7 Proliferation Assay

Cherdshewasart

Traisup

Picha³

2008

Journal of Reproduction and Development

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Abstract. The aim of this study was to evaluate the estrogenic activity of tuberous samples of wild, phytoestrogen-rich Pueraria mirifica collected from 28 out of 76 provinces of Thailand by MCF-7 proliferation assay. The plant extracts were administered to MCF-7, ERα positive human mammary adenocarcinoma cell cultures, for 3 days at dosages of 0.1, 1, 10, 100 and 1,000 µg/ml and were compared with 17β-estradiol at concentrations of 10 -12 -10 -6 M. The mean P. mirifica population at 1 µg/ml exhibited significant proliferation. Two plant samples exhibited levels of proliferation in MCF-7 that were similar to 17β-estradiol. The mean P. mirifica populations at 100 and 1,000 µg/ml exhibited significant cytotoxicity in MCF-7. Analysis of the estrogenic activity of puerarin, representative of major isoflavonoids in P. mirifica tubers, revealed proliferation in MCF-7 only at the highest dose (10 -6 M) that was 10 2 -10 5 times less active than 17β-estradiol. Puerarin and 17β-estradiol at concentration of 10 -12 -10 -6 M exhibited no cytotoxicity in MCF-7. Key words: Isoflavonoid, MCF-7 proliferation assay, Phytoestrogen, Pueraria mirifica, Puerarin (J. Reprod. Dev. 54: [63][64][65][66][67] 2008) he plants in genus Pueraria are largely distributed in Asia and have a long history of ethnopharmacological applications [1]. Pueraria mirifica Airy Shaw et Suvatabhandu, family Leguminosae, is an indigenous Thai herb. The potent estrogenic activity of the plant has been proven in a 1-year human clinical trial of 4 menopausal volunteers at Ramathibodi Hospital, Faculty of Medicine, Mahidol University, Bangkok, Thailand. An average dose of 100 mg of tuberous powder per day was effective for long-term treatment of menopausal symptoms [2], and toxicological tests showed that it did not have significant adverse effects in animals and human volunteers. An acute toxicity test of P. mirifica treatment at a dose of 16 g/kg BW using rats did not reveal any adverse effects [3]. However, P. mirifica did initiate adverse effects such as anemia, during subchronic treatment for 3 months at doses of 100 and 1,000 mg/kg BW [4]. Haemotological adverse effects were confirmed in a 6-month chronic toxicity test at a dose of 250 mg/kg BW [5]. Treatment with the plant extract at high doses 600 and 800 mg/kg BW, for 30 days induces micronucleus formation in male rats [6]. However, pretreatment of weanling rats with P. mirifica powder at a dose of 10, 100 and 1,000 mg/kg BW for 28 days resulted in a dose-dependent prevention of mammary tumors induced by 7, 12-dimethylbenz (a) anthracene (7, 12-DMBA) [7].The phytoestrogens of P. mirifica exhibit the following responses: dose-dependent estrogenic effects on the reproductive system, initiated vaginal cornification, increased uterine weight with decreased follicle stimulating hormone (FSH) and luteinizing hormone (LH) serum levels in ovariectomized rats [8], increased length of the follicular phase and total menstruation cycle [9], decreased estrogen, progesterone, FSH, and LH concentrations at the do...

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Phytochemical Analysis and Antibacterial Activity of Achillea coarctata Poir. Essential Oils

Stamenković

Petrović

Đorđević

2022

Chemistry & Biodiversity

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Essential oils obtained from different parts of Achillea coarctata species (inflorescences, stem and leaves and the whole aerial part) collected on four different locations in Serbia have been investigated to evaluate the chemical composition and antibacterial activity of isolated oils. The aim of this study was to determine differences in the chemical composition of essential oils obtained from different plant parts and how different type of substrate as well as different climate conditions affect the chemical composition of essential oils. Oxigenated terpenes were reported to be the major constituents in almost all examinated samples with 1,8cineole, caryophyllene oxide and cis-cadin-4-en-7-ol identified as dominant compounds. Disk diffusion assay was used to determine antibacterial activity against two Gram-positive (Bacillus subtilis subsp. spizizenii ATCC 6633 and Staphylococcus aureus ATCC 6538) and three Gram-negative bacteria (Escherichia coli ATCC 8739, Pseudomonas aeruginosa ATCC 9027 and Salmonella abony ATCC 6017). The obtained results showed that essential oils obtained from A. coarctata exhibit significant antibacterial activity against tested bacteria strains. The best inhibitory effect was observed against S. aureus, while on the other hand P. aeruginosa showed high level of resistance to almost all examined essential oils.

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In vitro estrogenic activity of Achillea millefolium L.

Cited by 85 publications

References 19 publications

Variability of phenolic compounds in flowers of Achillea millefolium wild populations in Lithuania

Variability of phenolic compounds in flowers of Achillea millefolium wild populations in Lithuania

Determination of the Estrogenic Activity of Wild Phytoestrogen-rich Pueraria mirifica by MCF-7 Proliferation Assay

Phytochemical Analysis and Antibacterial Activity of Achillea coarctata Poir. Essential Oils

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