In vitro estrogenicity of polybrominated flame retardants

Nakari, Tarja; Pessala, Piia

doi:10.1016/j.aquatox.2005.06.004

Cited by 44 publications

(17 citation statements)

References 37 publications

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“…Nakari and Pessala [29] reported that several flame retardants, including the main components in DE-71, are estrogenic. This conclusion was based on the observation that vitellogenin was synthesized and secreted by isolated rainbow trout liver cells in a primary hepatocyte assay in response to relatively low concentrations of BFRs (maximum induction was measured in response to BDE-99 at 10 µg/L, and 50 µg/L BDE47 and BDE153).…”

Section: Discussionmentioning

confidence: 99%

Effects of xenoestrogens on the expression of vitellogenin (vtg) and cytochrome P450 aromatase (cyp19aandb) genes in zebrafish (Danio rerio) larvae

Wang

Shi

et al. 2011

Journal of Environmental Science and Health, Part A

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Section: Discussionmentioning

confidence: 99%

Effects of xenoestrogens on the expression of vitellogenin (vtg) and cytochrome P450 aromatase (cyp19aandb) genes in zebrafish (Danio rerio) larvae

Wang

Shi

et al. 2011

Journal of Environmental Science and Health, Part A

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“…Some PBDEs and their hydroxylated or methoxylated metabolites have been found to exhibit estrogenic and anti-estrogenic activities in several studies in vitro and in vivo (Meerts et al 2001;Nakari and Pessala 2005;Dang et al 2007;Kojima et al 2009;Zhang et al 2011). However, little attention has been paid to the endocrine disrupting potential of these environmentally persistent contaminants in aquatic invertebrates, despite their presence in the environment and in the lipid tissues of such organisms at sometimes relatively high concentrations.…”

Section: Introductionmentioning

confidence: 99%

Polybrominated diphenyl ethers disrupt molting in neonatal Daphnia magna

Davies

Zou

2012

Ecotoxicology

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Polybrominated diphenyl ethers (PBDEs) are flame-retardants which can bioaccumulate and biomagnify and are found worldwide despite their banned usage in some countries. In recent years, the possibility that PBDEs may disrupt endocrine functions in vertebrates has been well investigated, but little attention has been paid to the endocrine disrupting potential in aquatic invertebrates. The current study aimed to investigate whether PBDEs affect molting in neonatal Daphnia magna. Prior to molting studies, 48 h LC50 values were tested for several environmentally prevalent PBDEs: PBDEs-28, -47, -99, -100 and -209. The 48 h LC50s determined were 110.7, 7.9, 2.6, and 11.1 μg/L for PBDEs-28, -47, -99, and -100, respectively, but the highest concentration of PBDEs-209 tested (2.5 mg/L) did not affect survival at 48 h. Sublethal concentrations of these were used to investigate their potential effects on molting, assessed by the time taken to reach 4 molts. Molting studies found that PBDE-28 at 12 μg/L significantly increased the time it took to complete 4 molts. PBDE-47 at 20 μg/L inhibited daphnid molting initially but such an inhibitory effect disappeared with the prolongation of exposure due to the death of sensitive individuals. No other PBDEs affected molting at the concentrations tested, while still maintaining relatively high survival rates. In conclusion, this study found that PBDEs-28 and -47 can delay molting at μg/L concentrations, which raises concern for disrupted molting in crustaceans exposed to PBDEs.

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“…Although there is not an extensive body of data regarding mechanisms of PBDE toxicity in fish, the structurallyrelated polychlorinated biphenyls induce oxidative stress as a mechanism of toxicity (Twaroski et al, 2001). Others have shown that hepatocytes isolated from rainbow trout are relatively sensitive to BDE 47 toxicity, and it has been hypothesized that such sensitivity may be partially dependent upon the poor detoxification capabilities of the cells (Nakari and Pessala, 2005). Although we did not investigate the effects of BDE 47 on expression of biotransformation or antioxidant genes in the trout liver or gill cells, others have demonstrated that a number of genes critical to cellular protection are down-regulated in the liver of trout exposed to BDE 47 in vivo (Hook et al, 2006).…”

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confidence: 99%

Comparative oxygen radical formation and toxicity of BDE 47 in rainbow trout cell lines

Shao

Eckert

Lee

et al. 2008

Marine Environmental Research

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The polybrominated diphenyl ethers (PBDEs) constitute a class of flame retardants whose residues have markedly increased in fish and human tissues during the last decade. In particular, the levels of certain PBDE congeners in salmon have raised concern regarding potential risks associated with dietary PBDE exposures. However, little is known regarding PBDE-mediated cell injury in relevant in vitro cell models. We conducted a comparative study of oxyradical production and cell injury in rainbow trout gill (RTgill-W1) and trout liver cells (RTL-W1) exposed to 2,2′,4,4′-tetrabromodiphenyl ether (BDE 47), a predominant BDE residue found in fish tissues such as salmonids. Exposure to low micromolar concentrations of BDE 47 elicited a significant loss in RTgill-W1 and RTL-W1 cell viability as measured by alamarBlue assay. The dose-response of BDE toxicity differed among the two cell lines, with the RTL-W1 liver cells showing greater resistance to toxicity at lower BDE 47 doses, but a more dramatic loss of viability relative to gill cells when challenged with higher (50 μM) doses. The sensitivity of the trout liver cells at higher BDE 47 exposures was reflected by a higher basal production of oxygen radical production by 6-carboxy-2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA) fluorescence that was markedly enhanced in the presence of BDE 47, suggesting an overwhelming of trout liver cell antioxidant defense pathways. Collectively, our data indicate that RTgill-W1 and RTL-W1 liver cells are sensitive to BDE 47-mediated cell injury through a mechanism that may involve oxidative stress. Our data also provide an in vitro basis for potential tissue differences in BDE 47-mediated cell injury.

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In vitro estrogenicity of polybrominated flame retardants

Cited by 44 publications

References 37 publications

Effects of xenoestrogens on the expression of vitellogenin (vtg) and cytochrome P450 aromatase (cyp19aandb) genes in zebrafish (Danio rerio) larvae

Effects of xenoestrogens on the expression of vitellogenin (vtg) and cytochrome P450 aromatase (cyp19aandb) genes in zebrafish (Danio rerio) larvae

Polybrominated diphenyl ethers disrupt molting in neonatal Daphnia magna

Comparative oxygen radical formation and toxicity of BDE 47 in rainbow trout cell lines

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