In vitro evaluation of a multi-layer radial-flow bioreactor based on galactosylated chitosan nanofiber scaffolds

Chu, Xuehui; Shi, Xiaolei; Feng, Zhang‐Qi; Gu, Jin-yang; Xu, Hao; Zhang, Yue; Gu, Zhongze; Ding, Yi-Tao

doi:10.1016/j.biomaterials.2009.05.020

Cited by 47 publications

(26 citation statements)

References 40 publications

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“…8B 1 and B 2 ), so that hepatocytes could not move together and detach from nanofibers to form aggregates and so formed even larger monolayered clusters than those on TCP. However, the nanofibers in meshRFs improved protein synthesis of hepatocytes as previously reported [34][35][36]41], so hepatocytes displayed better cell activity overall than those on TCP (Fig. 9B).…”

Section: Morphology and Activity Of Hepatocytessupporting

confidence: 55%

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Rat hepatocyte aggregate formation on discrete aligned nanofibers of type-I collagen-coated poly(l-lactic acid)

et al. 2010

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Section: Morphology and Activity Of Hepatocytessupporting

confidence: 55%

“…Rat primary hepatocytes were separated from 180 to 200 g male Sprague Dawley rats (Drum Tower Hospital, Nanjing, China) by a two-step perfusion method as previously described [34][35][36]. All harvests yielded hepatocytes with viability exceeding 90% by trypan-blue dye exclusion.…”

Section: Isolation and Culture Of Rat Primary Hepatocytesmentioning

confidence: 99%

Rat hepatocyte aggregate formation on discrete aligned nanofibers of type-I collagen-coated poly(l-lactic acid)

et al. 2010

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“…In vitro evaluation of the new system has completed, in which the culture medium RPMI 1640 was perfused and circulated in the bioreactor at 37°C and 5% CO 2 for dynamic detection of BAL efficacy. The results indicated high hepatocytic activity, good substance exchange, and satisfactory functions of biosynthesis and biotransformation [32]. For the moment, animal experiments are in progress.…”

Section: Current Application Of Balmentioning

confidence: 96%

“…Therefore, the physiochemical properties of various biomaterials were changed in all manner of ways, for example, by modifying and grafting. In recent years, the topology and especially the nanotopography structure of scaffolds were found to affect cell function to a great extent, because they mimicked the extracellular matrix and thereby offered the required microenvironment for cell growth [30][31][32].…”

Section: Bioreactor Designmentioning

confidence: 99%

Bioartificial liver devices: Perspectives on the state of the art

Ding

Shi

2010

Front. Med.

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Acute liver failure remains a significant cause of morbidity and mortality. Bioartificial liver (BAL) devices have been in development for more than 20 years. Such devices aim to temporarily take over the metabolic and excretory functions of the liver until the patients' own liver has recovered or a donor liver becomes available for transplant. The important issues include the choice of cell materials and the design of the bioreactor. Ideal BAL cell materials should be of good viability and functionality, easy to access, and exclude immunoreactive and tumorigenic cell materials. Unfortunately, the current cells in use in BAL do not meet these requirements. One of the challenges in BAL development is the improvement of current materials; another key point concerning cell materials is the coculture of different cells. The bioreactor is an important component of BAL, because it determines the viability and function of the hepatocytes within it. From the perspective of bioengineering, a successful and clinically effective bioreactor should mimic the structure of the liver and provide an in vivo-like microenvironment for the growth of hepatocytes, thereby maintaining the cells' viability and function to the maximum extent. One future trend in the development of the bioreactor is to improve the oxygen supply system. Another direction for future research on bioreactors is the application of biomedical materials. In conclusion, BAL is, in principle, an important therapeutic strategy for patients with acute liver failure, and may also be a bridge to liver transplantation. It requires further research and development, however, before it can enter clinical practice.

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“…Such nanofibrous mats, when hosting immobilised enzymes, combine simultaneously the biocatalytic function of enzymes and their separation from end products, performed with the help of a selective membrane, which is a typical mode for running continuous catalytic processes in enzymatic membrane-bioreactors [31][32][33]. Similarly, electrospun nanofibrous membrane have been involved recently in the fabrication of enzymatic membrane-bioreactors [34][35][36].…”

Section: Enzyme Immobilisationmentioning

confidence: 99%

Electrospinning-Based Nanobiosensors

Cesare

Macagnano

2015

Electrospinning for High Performance Sensors

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Biological interactions in biosensors occur through different mechanisms, on the basis of the type of biological receptor elements employed therein that confer the biological specificity to the biosensors themselves (biocatalytic, biocomplexing or bioaffinity biosensors). The use of different transduction systems (amperometric, potentiometric, field-effect transistors, piezometric and conductometric) defines the mode of detection. The interest and relevance of nanomaterials in the fabrication of nanobiosensors lie in their extraordinary properties that make them ideally suited and very promising for sensing applications. The resulting nanobiosensors are capable of sensing analytes in traces with fast, precise and accurate biological identification through miniaturised and easy to use systems. These advanced sensors are also characterised by lower detection limits, higher sensitivity values and high stability, and can further offer multi-detection possibilities. These exceptional features make nanobiosensors as the favourite tools in quality control, food safety, and traceability for their capacity of revealing and warning people against the presence of pathogens, toxins and pollutants, as well as bioterrorism agents. Despite the outstanding properties of these nanobiosensors, however, the efficiency of the biorecognition agent and the number of biorecognition sites available for interacting with target analytes can limit the sensitivity of this kind of sensors. The number of available biorecognition sites is directly related to the surface area of the sensor. Electrospinning technology can significantly increase the sensitivity of biosensors by replacing the typical planar interactive surface of conventional biosensors with a mat of electrospun nonwoven nanofibres, thereby taking advantage of the ultrahigh surface area offered by electrospun nanofibres. Moreover, adjusting the electrospinning process to produce

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In vitro evaluation of a multi-layer radial-flow bioreactor based on galactosylated chitosan nanofiber scaffolds

Cited by 47 publications

References 40 publications

Rat hepatocyte aggregate formation on discrete aligned nanofibers of type-I collagen-coated poly(l-lactic acid)

Rat hepatocyte aggregate formation on discrete aligned nanofibers of type-I collagen-coated poly(l-lactic acid)

Bioartificial liver devices: Perspectives on the state of the art

Electrospinning-Based Nanobiosensors

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