In Vitro Evaluation of Ag- and Sr-Doped Hydroxyapatite Coatings for Medical Applications

Ungureanu, Elena; Vlădescu, Alina; Parau, Anca Constantina; Mitran, Valentina; Cîmpean, Anișoara; Tarcolea, Mihai; Vrânceanu, Diana Maria; Cotruț, Cosmin Mihai

doi:10.3390/ma16155428

Cited by 15 publications

(8 citation statements)

References 144 publications

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“…A possible explanation for these low levels of ALP activity could be represented by a faster maturation process of the pre-osteoblast grown in contact with the surface of the newly designed materials into osteocytes, cells reported in the literature as expressing low ALP activity [50]. Similar results were noticed by our group in another study [51], where HA-based coatings led to a reduction in the ALP expression levels at 14 days post seeding in comparison to the control sample (flat Ti). Moreover, Genge et al [52] observed a direct correlation between the loss in the ALP activity and the accumulation of calcium inside the matrix vesicles, a phenomenon associated with the osteogenic differentiation process.…”

Section: Osteogenic Differentiation Capacitysupporting

confidence: 86%

In Vitro Studies on 3D-Printed PLA/HA/GNP Structures for Bone Tissue Regeneration

Negrescu,

Mocanu,

Miculescu

et al. 2024

Biomimetics

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The successful regeneration of large-size bone defects remains one of the most critical challenges faced in orthopaedics. Recently, 3D printing technology has been widely used to fabricate reliable, reproducible and economically affordable scaffolds with specifically designed shapes and porosity, capable of providing sufficient biomimetic cues for a desired cellular behaviour. Natural or synthetic polymers reinforced with active bioceramics and/or graphene derivatives have demonstrated adequate mechanical properties and a proper cellular response, attracting the attention of researchers in the bone regeneration field. In the present work, 3D-printed graphene nanoplatelet (GNP)-reinforced polylactic acid (PLA)/hydroxyapatite (HA) composite scaffolds were fabricated using the fused deposition modelling (FDM) technique. The in vitro response of the MC3T3-E1 pre-osteoblasts and RAW 264.7 macrophages revealed that these newly designed scaffolds exhibited various survival rates and a sustained proliferation. Moreover, as expected, the addition of HA into the PLA matrix contributed to mimicking a bone extracellular matrix, leading to positive effects on the pre-osteoblast osteogenic differentiation. In addition, a limited inflammatory response was also observed. Overall, the results suggest the great potential of the newly developed 3D-printed composite materials as suitable candidates for bone tissue engineering applications.

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Section: Osteogenic Differentiation Capacitysupporting

confidence: 86%

In Vitro Studies on 3D-Printed PLA/HA/GNP Structures for Bone Tissue Regeneration

Negrescu,

Mocanu,

Miculescu

et al. 2024

Biomimetics

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“…To the best of the authors' knowledge, most of the in vitro studies related to osteointegration properties of a biomaterial are still focused on the use of cell cultures in monolayer or direct and indirect co-cultures seeded onto biomaterials [24,26,[31][32][33] to evaluate the effects of tested materials or part of them on viability, morphology, gene/protein expression, and commitment; the set-up of complex tissue cultures mimicking implant osteointegration is still limited. Recently, Zankovic et al and Przekora et al performed human bone organ cultures with implanted biomaterials made of 3D-printed Ti6Al4V disks and chitosan/curdlan/hydroxyapatite for 28 and 46 days, respectively, in static conditions [34,35].…”

Section: Discussionmentioning

confidence: 99%

An Advanced Human Bone Tissue Culture Model for the Assessment of Implant Osteointegration In Vitro

Maglio,

Fini,

Sartori

et al. 2024

IJMS

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In the field of biomaterials for prosthetic reconstructive surgery, there is the lack of advanced innovative methods to investigate the potentialities of smart biomaterials before in vivo tests. Despite the complex osteointegration process being difficult to recreate in vitro, this study proposes an advanced in vitro tissue culture model of osteointegration using human bone. Cubic samples of trabecular bone were harvested, as waste material, from hip arthroplasty; inner cylindrical defects were created and assigned to the following groups: (1) empty defects (CTRneg); (2) defects implanted with a cytotoxic copper pin (CTRpos); (3) defects implanted with standard titanium pins (Ti). Tissues were dynamically cultured in mini rotating bioreactors and assessed weekly for viability and sterility. After 8 weeks, immunoenzymatic, microtomographic, histological, and histomorphometric analyses were performed. The model was able to simulate the effects of implantation of the materials, showing a drop in viability in CTR+, while Ti appears to have a trophic effect on bone. MicroCT and a histological analysis supported the results, with signs of matrix and bone deposition at the Ti implant site. Data suggest the reliability of the tested model in recreating the osteointegration process in vitro with the aim of reducing and refining in vivo preclinical models.

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“…Stanic and collaborators investigated the antimicrobial activity of Ag-Hydroxyapatite against microorganisms responsible for implant-related biofilms. The AFM analysis shows the morphological changes of the fungal or bacterial cells due to Ag-Hydroxyapatite activity [ 104 ]. A more recent study investigates the nano-interactions of CuI-TiO 2 NMs and fungal cells [ 5 ].…”

Section: Atomic Force and Holotomography Microscopy In Cancer Microbi...mentioning

confidence: 99%

Holotomography and atomic force microscopy: a powerful combination to enhance cancer, microbiology and nanotoxicology research

Medina-Ramirez,

Macias-Diaz,

Masuoka-Ito

et al. 2024

Discover Nano

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Modern imaging strategies are paramount to studying living systems such as cells, bacteria, and fungi and their response to pathogens, toxicants, and nanomaterials (NMs) as modulated by exposure and environmental factors. The need to understand the processes and mechanisms of damage, healing, and cell survivability of living systems continues to motivate the development of alternative imaging strategies. Of particular interest is the use of label-free techniques (microscopy procedures that do not require sample staining) that minimize interference of biological processes by foreign marking substances and reduce intense light exposure and potential photo-toxicity effects. This review focuses on the synergic capabilities of atomic force microscopy (AFM) as a well-developed and robust imaging strategy with demonstrated applications to unravel intimate details in biomedical applications, with the label-free, fast, and enduring Holotomographic Microscopy (HTM) strategy. HTM is a technique that combines holography and tomography using a low intensity continuous illumination laser to investigate (quantitatively and non-invasively) cells, microorganisms, and thin tissue by generating three-dimensional (3D) images and monitoring in real-time inner morphological changes. We first review the operating principles that form the basis for the complementary details provided by these techniques regarding the surface and internal information provided by HTM and AFM, which are essential and complimentary for the development of several biomedical areas studying the interaction mechanisms of NMs with living organisms. First, AFM can provide superb resolution on surface morphology and biomechanical characterization. Second, the quantitative phase capabilities of HTM enable superb modeling and quantification of the volume, surface area, protein content, and mass density of the main components of cells and microorganisms, including the morphology of cells in microbiological systems. These capabilities result from directly quantifying refractive index changes without requiring fluorescent markers or chemicals. As such, HTM is ideal for long-term monitoring of living organisms in conditions close to their natural settings. We present a case-based review of the principal uses of both techniques and their essential contributions to nanomedicine and nanotoxicology (study of the harmful effects of NMs in living organisms), emphasizing cancer and infectious disease control. The synergic impact of the sequential use of these complementary strategies provides a clear drive for adopting these techniques as interdependent fundamental tools. Graphical abstract

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In Vitro Evaluation of Ag- and Sr-Doped Hydroxyapatite Coatings for Medical Applications

Cited by 15 publications

References 144 publications

In Vitro Studies on 3D-Printed PLA/HA/GNP Structures for Bone Tissue Regeneration

In Vitro Studies on 3D-Printed PLA/HA/GNP Structures for Bone Tissue Regeneration

An Advanced Human Bone Tissue Culture Model for the Assessment of Implant Osteointegration In Vitro

Holotomography and atomic force microscopy: a powerful combination to enhance cancer, microbiology and nanotoxicology research

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