In Vitro Evaluation of Antibacterial and Antioxidant Properties of an Indian Medicinal Plant: Celastrus Paniculatus Willd

Abstract

Aim: To evaluate the antibacterial efficacy, the presence of different phytoconstituents of the leaf extracts of Indian plant Celastrus paniculatus against 6 antibiotic-resistant bacteria along with the evaluation of their antioxidant potentiality.
 Methods: The leaf extracts were prepared using 4 solvents, water, methanol, n-butanol, and acetone. The antibacterial property was evaluated using agar well diffusion and micro-broth dilution method. Qualitative phytochemical analysis was performed using standard protocols for each solvent extract to check for the presence of the secondary metabolites and phytochemicals which attributes to the antibacterial effectivity of C. paniculatus. Further, the antioxidant potentiality of all the 4 leaf-solvent-extracts was estimated using the DPPH method.
 Results: The n-butanol leaf extract exhibited the highest antibacterial and antioxidant activity, followed by acetone, methanol extracts. The aqueous extracts exhibited negligible antibacterial activity.
 Conclusion: This study validated the antibacterial properties of C. paniculatus leaf extracts against both gram-positive and negative bacteria and the phytochemical analysis revealed the presence of many secondary importance which can be attributed to its therapeutic properties. Hence, this plant can be further used as a complementary or alternative choice of drug for combatting multidrug-resistant bacterial pathogens.
 Keywords: Antibiotic resistance; Medicinal plants; Celastrus Paniculatus; Antibacterial activity, Phytochemical Analysis; Antioxidant activity