In Vitro Evaluation of Antifungal Sensitivity Assay of Biofield Energy Treated Fungi

Abstract

Fungi are the group of eukaryotic organisms such as yeast, mold, and mushrooms. The present work investigated the impact of biofield treatment on different pathogenic species of fungi in relation to antifungal sensitivity pattern. Each fungal sample was divided into three parts: C, control; T1, treatment (revived); T2 treatment (lyophilized). Treatment groups received the biofield treatment, and control group was remained as untreated. Mini-API ID32C strip employed for evaluation of antifungal sensitivity and minimum inhibitory concentration (MIC). The results showed that sensitivity of Candida albicans in T1 cells was changed against itraconazole from intermediate (I) to resistance (R) on day 10. The Candida kefyr exhibited a change in susceptibility against itraconazole in T2 cell from S→I, on day 10. Likewise, Candida krusei showed the alterations in sensitivity against two antifungal drugs: fluconazole from S→I (T1 on day 10) and itraconazole S→I (T1 and T2 on all assessment days). The Cryptococcus neoformans changed from S→I in T1 cell on day 5 and 10, against itraconazole. Sensitivity of Candida tropicalis was also altered from I→R against flucytosine (T1 and T2, on all assessment days). Similarly, Saccharomyces cerevisae altered from S→I (T1) and S→R (T2) on day 10. The MIC values of antifungal drugs were altered in the range of 2-8 folds, as compared to the control. Fungal identification data showed the significant changes in species similarity of few tested fungi as C. albicans changed from 91.9% to 98.5 and 99.9% in T1 and T2 cells, respectively on day 10. C. krusei was changed from 97.9% to 85.9% (T2 day 10), and C. tropicalis was altered from 88.7% to 99.6% (T1 day 5) and 99.0% (T2). These findings suggest that biofield treatment could be applied to alter the susceptibility pattern of antifungal drug therapy in future.

Impact of Consciousness Energy Healing Treatment on the Physicochemical and Thermal Properties of Magnesium Gluconate

“…[18]. The Trivedi Effect ® has gained the importance in several fields include material science [19,20], organic compounds [21,22], agricultural [23,24], microbiology [25,26], pharmaceuticals [27,28], nutraceuticals [29], biotechnology [30,31], genetics [32,33], medical [34] due to its astounding ability to alter the characteristic properties of the non-living and living objects. Scientific literature reported that The Trivedi Effect ® -Energy of Consciousness Healing Treatment has the astounding capability to alter physicochemical properties such as crystalline structure, crystallite size, particle size, surface area, and thermal stability of magnesium gluconate after treatment with different healers [35,36].…”

Structural Properties and Isotopic Abundance Ratio Analysis of Magnesium Gluconate Treated with the Energy of Consciousness Using LC-MS and NMR Spectroscopy

“…This process is known as The Trivedi Effect ® -Biofield Energy Healing Treatment [17] [18]. Biofield Energy Treatment (The Trivedi Effect ® ) has been drawn attention more in the recent times for its scientifically measurable capability to transform the characteristic properties of a wide varieties living and nonliving substances such as animals [19], cancer cells [20], microbes [21,22], plants [23,24], medium [25,26], materials [27,28], pharmaceuticals [29,30], nutraceuticals [31,32], organic compounds [33][34][35]. The scientific study indicated that Biofield Energy Healing Treatment (The Trivedi Effect ® ) might be an alternate method for increasing or decreasing the natural isotopic abundance ratio of the substances [36][37][38][39].…”