In vitro Evaluation of Antimicrobial and Cytotoxic Activities of Rosmarinus officinalis L. (Lamiaceae) Essential Oil Cultivated from South-West Tunisia

Abstract

Rosmarinus officinalis (Lamiaceae), commonly known as rosemary and iklil, is often used by North African populations for the treatment of several inflammatory and infectious diseases. This study aimed to investigate the antimicrobial and cytotoxic properties of essential oil extracted from the seeds and leaves of R. officinalis. Antimicrobial activity assays involved the determination of inhibition zones and the minimum inhibitory concentration with regards to sixteen pathogenic microbial strains, using disc diffusion and minimum inhibitory concentration methods. The oil showed excellent activity against Staphylococcus aureus, followed by Staphylococcus epidermidis and Staphylococcus aureus 25923, with strong inhibition zones of 38.00, 29.40 and 26.00 mm, respectively. Cytotoxicity assays involved the application of an MTT testing method against HeLa cell lines. The results yielded high IC 50 value values of up to 26,77 μg/ml. overall, the findings provided strong support for the strong candidacy of this plant for potential future application, particularly in the food and pharmaceutical industries, as a safe and costeffective natural additive to substitute toxic synthetic food additives.

Antioxidant, antimicrobial and antiproliferative activities of Anthemis palestina essential oil

“…Similarly, cytotoxic activities of essential oils from various aromatic plants were attributed to specific components of the oils [ 36 , 37 ]. Some of the main components of A. palestina oil have previously been tested for cytotoxic properties.…”

In vitro antimicrobial activity of extracts and essential oils ofCinnamomum,Salvia, andMenthaspp. against foodborne pathogens: A meta‐analysis study

“…After a rigorous assessment of relevant publications, 91 studies published since 2000 were identified as eligible for inclusion in this investigation (Abu‐Darwish et al., 2012; Akarca et al., 2019; Akdemir Evrendilek, 2015; Aliakbarlu et al., 2013; Al‐Nabulsi et al., 2020; Al‐Saghir, 2009; Alizadeh Sani et al., 2017; Andleeb et al., 2014; Awaisheh, 2013; Ayala‐Zavala et al., 2013; Baali et al., 2019; Bahadori et al., 2016; Bayoub et al., 2010; Bhavya et al., 2020; Bonilla & Sobral, 2016; Boukhebti et al., 2011; Bouyahya et al., 2019; Butkhup & Samappito, 2011; Campana et al., 2017; Cansian et al., 2010; Celikel & Kavas, 2008; Ceylan et al., 2014; de Oliveira et al., 2012; Deka et al., 2016; Djenane et al., 2012; Dobre et al., 2011; Eissa et al., 2012; El Abdouni Khayari et al., 2016; Elgayyar et al., 2001; Elshafie et al., 2016; El‐Shenawy et al., 2015; Feng et al., 2017; Fernández‐López et al., 2005; Ferreira et al., 2019; Fidan et al., 2019; Frank et al., 2018; Ghabraie et al., 2016; Golestani et al., 2015; Gonelimali et al., 2018; Goñi et al., 2009; Gupta et al., 2008; György, 2010; Hayouni et al., 2008; Huang et al., 2014; Hussein et al., 2018; Ibrahim, 2014; Chobba et al., 2012; Irkin & Korukluoglu, 2009; Iseppi et al., 2019; Iturriaga et al., 2012; Keskin et al., 2010; Kim et al., 2004, 2017; Kobus‐Cisowska et al.,…”

Rosmarinus plants: Key farm concepts towards food applications

“…Agar well diffusion method IZ = 20 mm (Tural & Turhan, 2017) Broth microdilution method MIC = 0.0313%, v/v, MBC = 0.0625%, v/v (Wang et al, 2012) Disc diffusion assay IZ = 7-38 mm, MIC = 25-75 μl/ml (Abdullah et al, 2015;Abu-Zaid et al, 2013;Chahboun et al, 2014;Djelloul, (Continues) & Hacini, 2017;Issabeagloo et al, 2012;Kazemi et al, 2012;Kesatebrhan & Tesema, 2014;Martins et al, 2012) Microdilution method IC 50 = 190 ± 5 μg/ml. MIC = 0.5-100 μg/ml, MBC = 1.5-5 μg/ml (Ben Chobba et al, 2012;Jardak et al, 2017;Kazemi et al, 2012) RAPD analysis-microdilution method MIC = 5 μl/ml, MBC = 1.25 μl/ml (Hamedo, 2009) Essential oil-rich fractions Disc diffusion assay and broth dilution assay IZ = 17-33 mm MBC = 0.25-2.25 mg/ml (Santonyo et al, 2005) reveal the potential of rosemary as a promising antimicrobial agent and foresee their effectiveness as a functional food. It is generally admitted that a substance with antimicrobial activity determined by the agar or disc diffusion method that displayed inhibition halo greater than 13 mm is active (Clinical and Laboratory Standards Institute, 2003).…”

“…56, 1.56, and 3.125 mg/ml, respectively (El Kichaoui et al, 2017) et al, 2012) Broth microdilution method MIC = 0.0625%, v/v, MBC = 0.125%, v/v(Wang et al, 2012) Disc diffusion assay MIC = 25 μl/ml(Abu-Zaid et al, 2013) IZ = 8-20 mm(Chahboun et al, 2014;Djelloul et al, 2017;Kazemi et al, 2012;Kesatebrhan & Tesema, 2014;Martins et al, 2012) RAPD analysis-microdilution method MIC = 25 μl/ml, MBC = 2.5 μl/ml(Hamedo, 2009) Essential oil-rich fractions Disc diffusion assay and broth dilution assay IZ = 17-33 mm, MBC = 2.25 to 0.25 mg/ml(Santonyo et al, 2005.5, 12.5, and 25 mg/ml, respectively(El Kichaoui et al, 2017) Essential oil Disc diffusion assay IZ = 12.8-15 mm(Ben Chobba et al, 2012;Kazemi et al, 2012;Martins et al, 2012) Microdilution method IC50 = 330 μg/ml, MIC = 3-282 μg/ml, 25, 12.5 and 50 mg/ml, respectively(El Kichaoui et al, 2017) .0313%, v/v; MBC = 0.25%, v/v(Wang et al, 2012) Essential oil Disc diffusion assay and microdilution method IZ = 20 mm and MIC = 4 μg/ml(Kazemi et al, 2012) Essential oil Disc diffusion assayIZ = 18-34 mm (Abdullah et al, 2015) Essential oil-rich fractions Disc diffusion assay and broth dilution assay IZ = 17 to 33 mm, MBC = 2.25 to 0.25 mg/ml(Santonyo et al, 2005) MIC = 6.25 mg/ml, MMC = 6.25 mg/ml(de Oliveira et al, 2017) …”