In Vitro Evaluation of Antioxidant Activity of Spondias Mombin Leaf Extract: Discovering Future Avenues for an Affordable and Efficient Antioxidant

Abstract

Spondias mombin is valued ethno medically in folkloric medicine. This medicinal plant according to traditional claim is said to cure various infectious and inflammatory ailments of gastrointestinal and genitourinary tract. Formation of reactive oxygen species (ROS) in inflammatory conditions is said to result in oxidative stress. The antibacterial and anti-inflammatory properties of Spondias mombin especially its leaves, have been linked to a range of compounds in it viz., anthraquinones, berberine, flavonoids, naphthoquinones, sesquiterpenes, quassiniods, indole and quinoline alkaloids. Hence, we aimed at exploring the antioxidant potential of the leaf extract of Spondias mombin by in vitro methods. The ethanolic extract of leaves was subjected to spectrophotometric analysis and DPPH methods to explore its reducing potential. The total phenolic and total flavonoid content was estimated by colorimetric methods. It was observed that ethanolic extract of Spondias mombin demonstrated a dose-dependent increase in the reducing property, with higher potency than the reference compound sodium metabisulphate. Spondias mombin shade dried leaf powder macerated and later extracted with hot water possessed a significantly high content of flavonoids than those extracted with other solvents. The leaf extract of Spondias mombin possesses significant antioxidant activity that may be attributed to its high flavonoid content which could afford protection in inflammatory conditions. This finding strengthens its widespread use in traditional medicine. Animal studies and clinical trials are necessary to validate these beneficial properties and translate them into clinical utility.

Antioxidant and Anti-inflammatory Activities of Extract and Fractions of Spondias mombin Leaf and Isolation of its Active Principles

“…The activity of compound 9 (IC50 = 11.8 μg/mL) was similar to that of the regular drug, ascorbic acid (IC50 = 9.4 μg/mL). This is in agreement with a study carried out 28 using the ethanol leaf extract of S. mombin, where the DPPH scavenging property was comparable with the regular drugascorbic acid. A positive result was also observed for the extracts/fractions of the leaf of S. mombin using in vitro DPPH scavenging assay.…”

Inhibitory Effects of Solvent-Partitioned Fractions of Two Nigerian Herbs (Spondias mombin Linn. and Mangifera indica L.) on α-Amylase and α-Glucosidase

“…Rice-Evans [ 51 ] reported that compounds with phenolic content could play an important role in eliminating radicals. The ABTS∙ scavenging property of the leaf might be due to the donating ability of the phenolics present in the fractions [ 52 , 53 , 54 ]. The antioxidant capacity of the leaves can be linked to their bioactive compounds, mainly antioxidant polyphenols, because of their ability to scavenge free radicals [ 55 ].…”

Comparative Impact of Solvent Extracts of Spondias mombin Leaves on In-vitro Antioxidant and Acetylcholinesterase Inhibitory Activities

“…This might be connected to how diverse the extracts from various solvents' secondary chemical profiles are characterized [36]. Accordingly, similar investigations conducted in the past [37,38] have demonstrated that extracting S. mombin with polar solvents improved its antioxidant capabilities when compared with reference antioxidant compounds.…”