In vitro Evaluation of ASCs and HUVECs Co-cultures in 3D Biodegradable Hydrogels on Neurite Outgrowth and Vascular Organization

Rocha, L.A.; Gomes, Eduardo D.; Afonso, João; Granja, Sara; Baltazar, Fátima; Silva, Nuno; Shoichet, Molly S.; Sousa, Rui A.; Learmonth, David A.; Salgado, António J.

doi:10.3389/fcell.2020.00489

Cited by 17 publications

(16 citation statements)

References 67 publications

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“…HUVECs were selected because they are the main cell type and play a critical role in the function of the blood vessel ( Yang et al, 2017 ; Kang et al, 2019 ; Rocha et al, 2020 ). Cell attachment is regarded as the first and critical response of cells with their surrounding bio-scaffold, which precedes all other cellular events, e.g., survival, viability, function, and differentiation ( Zhou et al, 2015 ; Zhou et al, 2020 ).…”

Section: Resultsmentioning

confidence: 99%

Preparation of Fucoidan-Based Electrospun Nanofibers and Their Interaction With Endothelial Cells

Chen

Zhu

Hao

et al. 2021

Front. Bioeng. Biotechnol.

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Sulfated polysaccharide fucoidan (FD) is widely applied in biomedical applications owing to its outstanding bioactivities. In addition to the biochemical features, the architecture of biomaterials plays a critical role in tissue repair and regeneration. Particularly, nanofibers have elicited great interest due to their extracellular matrix-like structure, high specific surface area, and favorable biological properties. Herein, chitosan-modified FD/ultra-high molecular weight polyethylene oxide (UHMWPEO) nanofibers are developed via green electrospinning and electrostatic interaction for studying their interaction with endothelial cells. The appropriate solvent is screened to dissolve FD. The electrospinnability of FD/UHMWPEO aqueous solutions is greatly dependent on the weight ratios of FD/UHMWPEO. The incorporation of UHMWPEO significantly improves the electrospinnability of solution and thermo-stability of nanofibers. Also, it is found that there is good miscibility or no phase separation in FD/UHMWPEO solutions. In vitro biological experiments show that the chitosan-modified FD/UHMWPEO nanofibers greatly facilitate the adhesion of endothelial cells and inhibit the attachment of monocytes. Thus, the designed FD-based nanofibers are promising bio-scaffolds in building tissue-engineered blood vessels.

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Section: Resultsmentioning

confidence: 99%

Preparation of Fucoidan-Based Electrospun Nanofibers and Their Interaction With Endothelial Cells

Chen

Zhu

Hao

et al. 2021

Front. Bioeng. Biotechnol.

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“…The supporting cells are able to modulate and produce ECM through secreted factors [ 76 , 77 ]. HUVEC was also used to evaluate the ability of angiogenesis [ 78 , 79 ]. Only the study by Tricinci [ 41 ] did not report the use of some type of ECM, substituted by the use of a synthetic scaffold as a similar function.…”

Section: Discussionmentioning

confidence: 99%

The Advances in Glioblastoma On-a-Chip for Therapy Approaches

Alves

Nucci

Mamani

et al. 2022

Cancers

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This systematic review aimed to verify the use of microfluidic devices in the process of implementing and evaluating the effectiveness of therapeutic approaches in glioblastoma on-a-chip, providing a broad view of advances to date in the use of this technology and their perspectives. We searched studies with the variations of the keywords “Glioblastoma”, “microfluidic devices”, “organ-on-a-chip” and “therapy” of the last ten years in PubMed and Scopus databases. Of 446 articles identified, only 22 articles were selected for analysis according to the inclusion and exclusion criteria. The microfluidic devices were mainly produced by soft lithography technology, using the PDMS material (72%). In the microenvironment, the main extracellular matrix used was collagen type I. Most studies used U87-MG glioblastoma cells from humans and 31.8% were co-cultivated with HUVEC, hCMEC/D3, and astrocytes. Chemotherapy was the majority of therapeutic approaches, assessing mainly the cellular viability and proliferation. Furthermore, some alternative therapies were reported in a few studies (22.6%). This study identified a diversity of glioblastoma on-a-chip to assess therapeutic approaches, often using intermediate levels of complexity. The most advanced level implemented the intersection between different biological systems (liver–brain or intestine–liver–brain), BBB model, allowing in vitro studies with greater human genetic similarity, reproducibility, and low cost, in a highly customizable platform.

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“…In 2020, Rocha et al demonstrated a synergistic effect of ASCs and HUVECs in a gellan gum hydrogel with a high potential for treating spinal cord injuries. HUVECs had only the ability to form vascular-like structures in the presence of ASCs [ 25 ].…”

Section: Discussionmentioning

confidence: 99%

“…Rocha and colleagues demonstrated that the assembly of vascular-like structures by HUVECs was improved in the presence of adipose stromal cells (ASCs), which shows the ability of these cells to reorganize the vascular milieu. The analysis of neuroregulatory factors showed that the co-cultivation of these cells upregulated the secretion of several neurotrophic factors relevant to trauma-related injuries [ 25 ].…”

Section: Introductionmentioning

confidence: 99%

Angiogenic Potential of Co-Cultured Human Umbilical Vein Endothelial Cells and Adipose Stromal Cells in Customizable 3D Engineered Collagen Sheets

Neßbach

Schwarz

Becke³

et al. 2022

JFB

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The wound healing process is much more complex than just the four phases of hemostasis, inflammation, proliferation, and maturation. Three-dimensional (3D) scaffolds made of biopolymers or ECM molecules using bioprinting can be used to promote the wound healing process, especially for complex 3D tissue lesions like chronic wounds. Here, a 3D-printed mold has been designed to produce customizable collagen type-I sheets containing human umbilical vein endothelial cells (HUVECs) and adipose stromal cells (ASCs) for the first time. In these 3D collagen sheets, the cellular activity leads to a restructuring of the collagen matrix. The upregulation of the growth factors Serpin E1 and TIMP-1 could be demonstrated in the 3D scaffolds with ACSs and HUVECs in co-culture. Both growth factors play a key role in the wound healing process. The capillary-like tube formation of HUVECs treated with supernatant from the collagen sheets revealed the secretion of angiogenic growth factors. Altogether, this demonstrates that collagen type I combined with the co-cultivation of HUVECs and ACSs has the potential to accelerate the process of angiogenesis and, thereby, might promote wound healing.

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In vitro Evaluation of ASCs and HUVECs Co-cultures in 3D Biodegradable Hydrogels on Neurite Outgrowth and Vascular Organization

Cited by 17 publications

References 67 publications

Preparation of Fucoidan-Based Electrospun Nanofibers and Their Interaction With Endothelial Cells

Preparation of Fucoidan-Based Electrospun Nanofibers and Their Interaction With Endothelial Cells

The Advances in Glioblastoma On-a-Chip for Therapy Approaches

Angiogenic Potential of Co-Cultured Human Umbilical Vein Endothelial Cells and Adipose Stromal Cells in Customizable 3D Engineered Collagen Sheets

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