In vitro evaluation of bilayer membranes of PLGA/hydroxyapatite/β-tricalcium phosphate for guided bone regeneration

Santos, Vivian Inês dos; Merlini, Claudia; Aragones, Águedo; Cesca, Karina; Fredel, Márcio C.

doi:10.1016/j.msec.2020.110849

Cited by 41 publications

(40 citation statements)

References 36 publications

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“…Some inorganic materials have been used in the regeneration of damaged nerves, such as metallic NPs [ 108 ], silica NPs [ 109 ], magnetic NPs [ 110 ] and quantum dots [ 111 ]. On the other hand, distinctive organic nanomaterials have been studied for neural tissue regeneration applications [ 112 ]. Polymeric NPs [ 113 ], liposomes [ 114 ], dendrimers [ 115 ], micelles [ 116 ], nanofibres [ 117 ] and carbon-based nanomaterials [ 118 ] are some natural nanomaterials.…”

Section: Nanomaterials For Biomedical Applicationsmentioning

confidence: 99%

Nanomaterials for Biomedical Applications: Production, Characterisations, Recent Trends and Difficulties

et al. 2021

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Designing of nanomaterials has now become a top-priority research goal with a view to developing specific applications in the biomedical fields. In fact, the recent trends in the literature show that there is a lack of in-depth reviews that specifically highlight the current knowledge based on the design and production of nanomaterials. Considerations of size, shape, surface charge and microstructures are important factors in this regard as they affect the performance of nanoparticles (NPs). These parameters are also found to be dependent on their synthesis methods. The characterisation techniques that have been used for the investigation of these nanomaterials are relatively different in their concepts, sample preparation methods and obtained results. Consequently, this review article aims to carry out an in-depth discussion on the recent trends on nanomaterials for biomedical engineering, with a particular emphasis on the choices of the nanomaterials, preparation methods/instruments and characterisations techniques used for designing of nanomaterials. Key applications of these nanomaterials, such as tissue regeneration, medication delivery and wound healing, are also discussed briefly. Covering this knowledge gap will result in a better understanding of the role of nanomaterial design and subsequent larger-scale applications in terms of both its potential and difficulties.

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Section: Nanomaterials For Biomedical Applicationsmentioning

confidence: 99%

Nanomaterials for Biomedical Applications: Production, Characterisations, Recent Trends and Difficulties

et al. 2021

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“…Scaffolds can be manufactured by conventional or additive manufacturing techniques and more recently, by 3D and 4D printing techniques [145,146]. Conventional techniques include methods such as solvent casting and particle leaching, freeze-drying, thermally induced phase separation, gas foaming, powder-forming, polymeric sponge replica method, and electrospinning [145,[147][148][149][150], while among additive manufacturing techniques stereolithography, fused-deposition modeling, selective stand out laser sintering and electron beam melting stand out [145,151].…”

Section: Techniques For Manufacturing Scaffoldsmentioning

confidence: 99%

“…Thermally-induced phase separation [148,157] A polymer is dissolved in a solvent at high temperature, followed by rapid cooling. The solvent is separated from the polymeric structure due to the change in the solubility coefficient caused by the temperature reduction, forming one phase rich in polymer and another poor.…”

Section: Conventional Techniquesmentioning

confidence: 99%

Materials and Manufacturing Techniques for Polymeric and Ceramic Scaffolds Used in Implant Dentistry

et al. 2021

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Preventive and regenerative techniques have been suggested to minimize the aesthetic and functional effects caused by intraoral bone defects, enabling the installation of dental implants. Among them, porous three-dimensional structures (scaffolds) composed mainly of bioabsorbable ceramics, such as hydroxyapatite (HAp) and β-tricalcium phosphate (β-TCP) stand out for reducing the use of autogenous, homogeneous, and xenogenous bone grafts and their unwanted effects. In order to stimulate bone formation, biodegradable polymers such as cellulose, collagen, glycosaminoglycans, polylactic acid (PLA), polyvinyl alcohol (PVA), poly-ε-caprolactone (PCL), polyglycolic acid (PGA), polyhydroxylbutyrate (PHB), polypropylenofumarate (PPF), polylactic-co-glycolic acid (PLGA), and poly L-co-D, L lactic acid (PLDLA) have also been studied. More recently, hybrid scaffolds can combine the tunable macro/microporosity and osteoinductive properties of ceramic materials with the chemical/physical properties of biodegradable polymers. Various methods are suggested for the manufacture of scaffolds with adequate porosity, such as conventional and additive manufacturing techniques and, more recently, 3D and 4D printing. The purpose of this manuscript is to review features concerning biomaterials, scaffolds macro and microstructure, fabrication techniques, as well as the potential interaction of the scaffolds with the human body.

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“…With the rise of biological tissue engineering, a large number of new materials such as active bioceramics, hydrogels, and PLGA have been developed. Among them, PLGA has been the focus of tissue engineering research in recent years, especially the research on bone and cartilage regeneration, because it has trustworthy mechanical strength and is easy to prepare into the desired shape (Canton et al, 2010;Dos Santos et al, 2020;Ginebra et al, 2018;Ribeiro et al, 2018;Wang et al, 2019). In addition, many studies have shown that rapamycin promotes osteogenesis by inhibiting m-TORC1 (Barsh et al, 2015;Li et al, 2017;Wu et al, 2019).…”

Section: Immunohistochemical Assessmentmentioning

confidence: 99%

TCP/PLGA composite scaffold loaded rapamycin in situ enhances lumbar fusion by regulating osteoblast and osteoclast activity

Hai

Zhu

Cheng

et al. 2021

J Tissue Eng Regen Med

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The purpose of this study was to develop a novel β-tricalcium phosphate (TCP)/poly (D,L-lactic-co-glycolic acid) (PLGA) composite scaffold loaded with rapamycin that can regulate the activity of osteoblasts and osteoclasts for lumbar fusion. The TCP/ PLGA composite scaffold was fabricated by cryogenic three-dimensional printing techniques and then loaded with rapamycin in situ. The structural surface morphology of the composite scaffold was tested with scanning electron microscope. To evaluate the biocompatibility of the composite scaffold in vitro, bone marrow mesenchymal stem cells (BMSCs) were cultured on the TCP/PLGA composite scaffold slide and tested with Live/Dead Viability Kit. The effect of rapamycin on osteoclast and osteoblast was studied with staining and Western blotting. The in vitro results showed that the rapamycin-loaded TCP/PLGA composite scaffold showed good biocompatibility with BMSC and released rapamycin obviously promoted the osteoblast differentiation and mineralization. In vivo study, the TCP/ PLGA composite scaffold loaded with rapamycin were implanted in lumbar fusion model and study with micro-computed tomography scanning, hematoxylin-eosin, Masson, and immune-histological staining, to evaluate the effect of rapamycin on bone fusion. The in vivo results demonstrated that rapamycin-loaded TCP/PLGA composite scaffold could enhance bone formation by regulating osteoblast and osteoclast activity, respectively. In this study, the TCP/PLGA composite scaffold loaded with rapamycin was confirmed to provide great compatibility and improved performance in lumbar fusion by regulating osteoblastic and osteoclastic activity and would be a promising composite biomaterial for bone tissue engineering.

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In vitro evaluation of bilayer membranes of PLGA/hydroxyapatite/β-tricalcium phosphate for guided bone regeneration

Cited by 41 publications

References 36 publications

Nanomaterials for Biomedical Applications: Production, Characterisations, Recent Trends and Difficulties

Nanomaterials for Biomedical Applications: Production, Characterisations, Recent Trends and Difficulties

Materials and Manufacturing Techniques for Polymeric and Ceramic Scaffolds Used in Implant Dentistry

TCP/PLGA composite scaffold loaded rapamycin in situ enhances lumbar fusion by regulating osteoblast and osteoclast activity

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