“In vitro” evaluation of bone marrow angiogenesis in myelodysplastic syndromes: a morphological and functional approach

Campioni, Diana; Punturieri, Marina; Bardi, Antonella; Moretti, Sabrina; Tammiso, Elisa; Lanza, Francesco; Castoldi, Gianluigi

doi:10.1016/s0145-2126(03)00123-1

Cited by 17 publications

(13 citation statements)

References 41 publications

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“…In recent years, based on the analysis of the MVD in fixed human BM biopsies and mouse models, some authors have investigated the presence of endothelial cells in relation to the angiogenesis process; [11][12][13][14][15] in some cases, angiogenesis has been found to be increased in MM, 11 AML, 13 and MDS. 15,20 Moreover, it has been shown that the levels of some angiogenic factors such as VEGF could mediate leukemic cell proliferation, and thus may be considered prognostic factors in acute leukemia. 13,21 As far as the analysis of NHL patients is concerned, high serum VEGF levels were associated with poor outcome 22 and circulating IL-6, VEGF and b-FGF (but not of endostatin, angiogenin, and leptin) might be considered prognostic factors in NHL.…”

Section: Discussionmentioning

confidence: 99%

In vitro assessment of bone marrow endothelial colonies (CFU-En) in non-Hodgkin's lymphoma patients undergoing peripheral blood stem cell transplantation

Lanza

Campioni

Punturieri

et al. 2003

Bone Marrow Transplant

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Section: Discussionmentioning

confidence: 99%

In vitro assessment of bone marrow endothelial colonies (CFU-En) in non-Hodgkin's lymphoma patients undergoing peripheral blood stem cell transplantation

Lanza

Campioni

Punturieri

et al. 2003

Bone Marrow Transplant

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“…Cultures were performed in triplicate and the detection of adherent colonies (id, aggregates with more than 50 cells) was monitored and scored as either EPC/ECFC or CFU-EC on the basis of morphological features, as previously described [6], [25]–[27].…”

Section: Methodsmentioning

confidence: 99%

“…Immunohistochemistry analysis was carried out by performing the alkaline phosphatase anti-alkaline-phosphatase (APAAP) staining, as previously described [26], [27], using the following monoclonal Ab: Factor VIII Ab (F8/86; DAKO), CD31 (WM-59; BD), VEGF receptor-2 (KDR-1; Sigma-Chemical, St. Louis, MO), CD105 (8E11; Cymbus Biotechnology); CD106 (1G1b1; Valter Occhiena, Torino, Italy), CD14 (MΦP9; BD), and CD45 (HI30; BD). EPC/ECFC were identified on the basis of their positivity for: Factor VIII, CD31, VEGFR-2, CD105, CD106, and negativity for CD14 and CD45 markers.…”

Section: Methodsmentioning

confidence: 99%

In Vitro Characterization of Circulating Endothelial Progenitor Cells Isolated from Patients with Acute Coronary Syndrome

et al. 2013

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BackgroundThe current understanding of the functional characteristics of circulating endothelial progenitor cells (EPC) is limited, especially in patients affected by cardiovascular diseases. In this study, we have analyzed the in vitro clonogenic capacity of circulating EPC, also known as endothelial colony-forming cells (ECFC), in patients with acute coronary syndrome (ACS), in comparison to the colony forming unit-endothelial-like cells (CFU-EC) of hematopoietic/monocytic origin.Methodology/Principal FindingsBy culturing peripheral blood mononuclear cells (PBMC) of patients with ACS (n = 70), CFU-EC were frequently isolated (from 77% of ACS patients), while EPC/ECFC were obtained only in a small subset (13%) of PBMC samples, all harvested between 7–14 days after the acute cardiovascular event. Notably, ex-vivo generation of EPC/ECFC was correlated to a higher in vitro release of PDGF-AA by the corresponding ACS patient PBMC. By using specific endothelial culture media, EPC/ECFC displayed in vitro expansion capacity, allowing the phenotypic and functional characterization of the cells. Indeed, after expansion, EPC/ECFC exhibited a normal diploid chromosomal setting by FISH analysis and an immunophenotype characterized by: i) uniform positivity for the expression of CD105, CD31, CD146 and Factor VIII, i) variable expression of the CD34, CD106 and CD184 markers, and iii) negativity for CD45, CD90, CD117 and CD133. Of interest, in single-cell replanting assays EPC/ECFC exhibited clonogenic expansion capacity, forming secondary colonies characterized by variable proliferation capacities.Conclusion/SignificanceOur data indicate that a careful characterization of true EPC is needed in order to design future studies in the clinical autologous setting of patients with ACS.

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“…An angiogenesis switch has been proposed as one of the mechanisms of the progression of MDS to acute leukemia. However, although an increased microvascular density has been observed in most studies of MDS, there are conflicting data about its increase 33,34 or not 35,36 during the transformation to overt acute leukemia. In a recent analysis of this issue, vascular density and expression of basic FGF, angiopoietins, VEGFR2 and Tie2 were lower in MDS transformed to leukemia than in de novo AML, suggesting a certain independence of angiogenesis in the late phase of leukemic evolution.…”

Section: Neoangiogenesis and Acute Leukemiamentioning

confidence: 97%

Contribution of bone microenvironment to leukemogenesis and leukemia progression

et al. 2009

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Tumor microenvironment has a major role in cancer progression and resistance to treatment. The bone marrow (BM) is a dynamic network of growth factors, cytokines and stromal cells, providing a permissive environment for leukemogenesis and progression. Both BM stroma and leukemic blasts promote angiogenesis, which is increased in acute lymphoblastic leukemia and acute myeloid leukemia. Growth factors like vascular endothelial growth factor (VEGF), basic fibroblast growth factor and angiopoietins are the main proangiogenic mediators in acute leukemia. Autocrine proleukemic loops have been described for VEGF and angiopoietin in hematopoietic cells. Interactions of stromal cells and extracellular matrix with leukemic blasts can also generate antiapoptotic signals that contribute to neoplastic progression and persistence of treatment-resistant minimal residual disease. High expression of CXC chemokine ligand 4 (CXCR4) by leukemic blasts and activation of the CXCR4–CXCL12 axis is involved in leukemia progression and disruption of normal hematopoiesis. Leukemia-associated bone microenvironment markers could be used as prognostic or predictive indicators of disease progression and/or treatment outcome. Studies related to bone microenvironment would likely provide a better understanding of the treatment resistance associated with leukemia therapy and design of new treatments.

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“In vitro” evaluation of bone marrow angiogenesis in myelodysplastic syndromes: a morphological and functional approach

Cited by 17 publications

References 41 publications

In vitro assessment of bone marrow endothelial colonies (CFU-En) in non-Hodgkin's lymphoma patients undergoing peripheral blood stem cell transplantation

In vitro assessment of bone marrow endothelial colonies (CFU-En) in non-Hodgkin's lymphoma patients undergoing peripheral blood stem cell transplantation

In Vitro Characterization of Circulating Endothelial Progenitor Cells Isolated from Patients with Acute Coronary Syndrome

Contribution of bone microenvironment to leukemogenesis and leukemia progression

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