In vitro evaluation of cell/biomaterial interaction by MTT assay

Ciapetti, G.; Cenni, E.; Pratelli, Loredana; Pizzoferrato, Alberto

doi:10.1016/0142-9612(93)90055-7

Cited by 418 publications

(250 citation statements)

References 23 publications

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“…An advantage of the MTT assay is that it can identify changes in metabolism and cell function from contact within cells and materials, even in the absence of dead cells (20). Additionally, it is a low-cost procedure that yields results within 48 h (12,13,18,19), and is therefore the most commonly used procedure for determining the cytotoxicity of different types of materials (20).…”

Section: Discussionmentioning

confidence: 99%

“…Additionally, it is a low-cost procedure that yields results within 48 h (12,13,18,19), and is therefore the most commonly used procedure for determining the cytotoxicity of different types of materials (20). Due to these characteristics and the endorsement by some authors (13,15,(21)(22)(23)(24)(25), this assay was used in this study for assessing the cytotoxicity of all the six endodontic sealers.…”

Section: Discussionmentioning

confidence: 99%

“…The MTT assay was used to assess cell viability (12,13,15,18,20,(22)(23)(24)(25). The MTT reduction tests were performed in quadruplicate at 12, 24, and 72 h using 96-well cell culture microplates.…”

Section: Discussionmentioning

confidence: 99%

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Cytotoxicity of root canal sealers on endothelial cell cultures

et al. 2013

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This study evaluated, in vitro, the cytotoxicity of six root canal sealers after 12, 24 and 72 h of contact time, using an endothelial ECV-304 cell line. The MTT assay was used for analysis of cell viability. Twelve specimens of each sealer were prepared and randomly assigned to 6 groups according to the commercial brands (n=4/time). A control group was also formed, which was not subjected to the contact with sealers. To assess the effects of sealers on endothelial cells, the specimens were placed in culture plate wells and incubated at 37°C with 5% CO 2 and 100% humidity. MTT assays were performed in quadruplicate after 12, 24 and 72 h of contact of the sealer specimens with monolayers. Statistical analysis was performed by two-way ANOVA with Bonferroni post-hoc test at a significance level of 5%. Analysis of absorbance in the experimental groups showed that GuttaFlow presented the lowest cytotoxicity, with a mean absorbance of 0.048, followed by Pulp Canal Sealer (0.038), Sealer 26 (0.038), Endo Densell (0.036) and Pulp Fill (0.035). The control group had a mean absorbance of 0.098. Based on the results, Endofill and GuttaFlow were the most and the least cytotoxic sealers, respectively.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Cytotoxicity of root canal sealers on endothelial cell cultures

et al. 2013

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“…This assay utilizes mitochondrial dehydrogenase activity that results in cleavage of a yellow tetrazolium salt (MTT) to form purple formazan crystals [132]. MTT dye reduction appears to take place only in viable cells; in contrast, nonviable cells cannot reduce the dye [133].…”

Section: Biocompatibilitymentioning

confidence: 99%

“…Ciapetti et al [134] recommended a model proposed in xenobiotic research. It utilizes the LDH assay for evaluation of membrane integrity, 3 H-thymidine uptake to quantify DNA synthesis, neutral red uptake to evaluate the efficiency of lysosomes, the Lowry method for protein assessment, and the MTT assay to test mitochondrial activity.…”

Section: Biocompatibilitymentioning

confidence: 99%

Reinforcement of flowable dental composites with titanium dioxide nanotubes

et al. 2016

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Cardiac tissue engineering: Cell seeding, cultivation parameters, and tissue construct characterization

Papadaki

Rupnick

Schoen

et al. 1999

Biotechnol. Bioeng.

493

322

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Cardiac tissue engineering has been motivated by the need to create functional tissue equivalents for scientific studies and cardiac tissue repair. We previously demonstrated that contractile cardiac cell–polymer constructs can be cultivated using isolated cells, 3‐dimensional scaffolds, and bioreactors. In the present work, we examined the effects of (1) cell source (neonatal rat or embryonic chick), (2) initial cell seeding density, (3) cell seeding vessel, and (4) tissue culture vessel on the structure and composition of engineered cardiac muscle. Constructs seeded under well‐mixed conditions with rat heart cells at a high initial density ((6–8) × 106 cells/polymer scaffold) maintained structural integrity and contained macroscopic contractile areas (approximately 20 mm2). Seeding in rotating vessels (laminar flow) rather than mixed flasks (turbulent flow) resulted in 23% higher seeding efficiency and 20% less cell damage as assessed by medium lactate dehydrogenase levels (p < 0.05). Advantages of culturing constructs under mixed rather than static conditions included the maintenance of metabolic parameters in physiological ranges, 2–4 times higher construct cellularity (p ≤ 0.0001), more aerobic cell metabolism, and a more physiological, elongated cell shape. Cultivations in rotating bioreactors, in which flow patterns are laminar and dynamic, yielded constructs with a more active, aerobic metabolism as compared to constructs cultured in mixed or static flasks. After 1–2 weeks of cultivation, tissue constructs expressed cardiac specific proteins and ultrastructural features and had approximately 2–6 times lower cellularity (p < 0.05) but similar metabolic activity per unit cell when compared to native cardiac tissue. © 1999 John Wiley & Sons, Inc. Biotechnol Bioeng 64: 580–589, 1999.

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In vitro evaluation of cell/biomaterial interaction by MTT assay

Cited by 418 publications

References 23 publications

Cytotoxicity of root canal sealers on endothelial cell cultures

Cytotoxicity of root canal sealers on endothelial cell cultures

Reinforcement of flowable dental composites with titanium dioxide nanotubes

Cardiac tissue engineering: Cell seeding, cultivation parameters, and tissue construct characterization

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