In vitro evaluation of copper tolerance and accumulation in Populus nigra

Vuksanović, Vanja; Kovačević, Branislav; Katanić, Marina; Orlović, Saša; Miladinović, Dragana

doi:10.2298/abs170210014v

Cited by 7 publications

(4 citation statements)

References 26 publications

(32 reference statements)

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“…High concentrations of HM such as cadmium (Cd), lead (Pb), chromium (Cr), copper (Cu), and mercury (Hg) produce toxic effects on soil processes [3]. To eliminate HM from contaminated soils, metal-accumulator plants have been used as a novel eco-friendly and cost-effective approach, referred to as phytoremediation [4].…”

Section: Introductionmentioning

confidence: 99%

Biochar improves the morphological, physiological and biochemical properties of white willow seedlings in heavy metal-contaminated soil

Mokaram-Kashtiban

Hosseini

Kouchaksaraei

et al. 2019

Arch biol sci (Beogr)

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Biochar is an efficient soil amendment used for promoting plant resistance to heavy metal (HM)-contaminated soils. There is a need for further investigation of its impacts on plants and soil. This study was undertaken as a pot experiment to assess the effect of biochar (0, 2.5, and 5% mass fractions) on the morphological, physiological and biochemical responses of white willow seedlings (Salix alba L.) cultured in uncontaminated soil and mixed soil contaminated with HM (Cu, Pb, and Cd). Additionally, some chemical properties and HM bioavailability were evaluated. Biochar increased height and diameter, root elongation, leaf area and dry biomass of the seedlings in both soils. Its addition to the contaminated soil reduced electrolyte leakage, the malondialdehyde and proline contents but increased the chlorophyll content, net photosynthesis rate, intercellular CO 2 concentration and transpiration rate in the leaf. Use of biochar (especially at 5% rate) in both soils, increased soil pH, total nitrogen, soil organic carbon and available P and K, while in the contaminated soil the availability of Cu, Pb, and Cd decreased. The results showed that biochar is a suitable amendment to contaminated soils that improves plant properties by improving soil chemical features and immobilizing HMs.Abbreviations: Brunauer-Emmett-Teller (BET); Barrett-Joyner-Halenda (BJH); intercellular CO 2 concentration (Ci); transpiration rate (E); electrolyte leakage (El); stomatal conductance (Gs); leaf area (LA); heavy metal (HM); malondialdehyde (MDA); total nitrogen (TN); scanning electron microscopy (SEM); specific leaf area (SLA); soil organic carbon (SOC); water use efficiency (WUE) Arch Biol Sci. 2019;71(2):281-291 https://doi.

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Section: Introductionmentioning

confidence: 99%

Biochar improves the morphological, physiological and biochemical properties of white willow seedlings in heavy metal-contaminated soil

Mokaram-Kashtiban

Hosseini

Kouchaksaraei

et al. 2019

Arch biol sci (Beogr)

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“…In comparison with the results gained by Kovačević et al (2013a) on media with pH 5.5, it can be assumed that a stronger effect of lead in our work was achieved by lowering pH and using citric acid. Likewise, Vuksanović et al (2017b) gained toxic effect and total absence of rooting of Populus nigra in medium with pH 3 and 10 -3 M Cu 2+ , while di Lonardo et al (2011) reported absence of toxic effect on Populus alba for the same concentration of copper in medium with pH 5.2.…”

Section: Discussionmentioning

confidence: 93%

In Vitro Lead Tolerance Testing in White Poplar Genotypes on Acidic Medium

Kovačević

Tišma

Nikolić

et al. 2020

SEEFOR

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This study evaluates in vitro tolerance of white poplar genotypes to the presence of lead in an acidic rooting medium. Lead was supplemented in form of Pb(NO3)2, in the following concentrations: 0 M (Control), 10-6 M, 10-5 M, 10-4 M and 10-3 M. After four weeks of cultivation, the following characters were measured: number of roots, the length of the longest root, the shoot height, dry root mass and dry shoot mass, and parameters related to photosynthetic pigments: content of chlorophyll a, b, a+b, and of carotenoids in fresh mass, as well as chlorophyll a/b ratio. For further statistical analysis, tolerance indices by Turner and Marshal (TI) were calculated for each measured character. The strongest inhibitory effect was achieved on the medium with 10-4 M Pb(NO3)2, but the best differentiation between genotypes was achieved on the medium with 10-5 M Pb(NO3)2. The highest tolerance indices for the length of the longest root and shoot height had genotypes L-12 and LBM, and for root and shoot dry mass genotypes LCM and L-12. There were no significant differences between genotypes in tolerance indices by any of the examined photosynthetic parameters. The obtained results suggest that both low pH and the presence of citric acid as chelating agent improved evaluation of lead tolerance in comparison with the results obtained on media with standard pH and without citric acid in similar studies. In vitro tests on acidic medium with citric acid can efficiently differentiate examined genotypes for lead tolerance, which could be important for their use in phytoextraction projects, especially on acidic soils.

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“…This is the first study to demonstrate evidence of Cu 2+ effects on morphological, physiological and biochemical variables in C. quitensis. Vuksanović et al (2017) [39] study five different European black poplar (Populusnigra L.) genotypes were studied in vitro for their tolerance to Cu 2+ based on morphological parameters, biomass accumulation and pigment content, as well as Cu 2+ accumulation in aboveground plant parts. Also, the effect of the pH of the medium and Cu 2+ concentration was examined in order to optimize the evaluation of Cu 2+ tolerance in vitro.…”

Section: Discussionmentioning

confidence: 99%

The Potential Role of Cupper and Combined Action with IAA on Tolerance Strategy of Two Broad Bean Cultivars

El-Samad¹,

Shaddad²,

El-Hakeem³

2018

AJPS

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The present work was conducting to study the strategy response of two broad bean cultivars Assiut 84 and Assiut 125 to different Cu 2+ concentrations 100 ppm, 200 ppm, 300 ppm and 350 ppm in addition to control and interactions with IAA treatments. The dry matter exhibited the biphasic effect of Cu 2+ on the growth criteria of the two broad bean cultivars. While the lower and moderate doses of Cu 2+ (100 ppm and 200 ppm) stimulated the growth of the two cultivars, the higher doses revealed the opposite event where the growth dropped in both cultivars. This effect was more pronounced in cv. Assiut 84 than in cv. Assiut 125 and also at the higher Cu 2+ concentration the growth dropped slightly in cv. Assiut 84 and highly significantly in cv. Assiut 125. The percent of increase in dry matter at 200 ppm in stem and leaf of cv. Assiut 84 was 120.45% and 155.31%, otherwise this percent of increase in these organs of cv. Assiut 125 was 114.29% and 131.41%. However the percent of reduction at 350 ppm Cu 2+ in root and stem of cv. Assiut 84 was 74.13%, 79.23% and in root, stem and leaf of cv. Assiut 125 was 59.27%, 70.91%, 70.76% compared with control plants. Soluble carbohydrate in cv. Assiut 84 and cv. Assiut 125 was markedly increased while soluble protein was decreased in root, stem and in leaves at lower Cu 2+ concentration. Also while Cu 2+-stressed cv. Assiut 84 maintained potassium and magnesium levels around the control values and some promotion occurred especially in roots and stems, these cations dropped markedly in cv. Assiut 125 as a result of Cu 2+ treatments. While Cu 2+ had a marked stimulatory effect in the absorption and accumulation of calcium in the different organs of cv. Assiut 84, it, on the other hand, significantly inhibited the accumulation of this cation in the different organs of cv. Assiut 125. Treatments broad bean cultivars with Cu 2+ plus IAA induced an increase in growth parameters, soluble suga, so-How to cite this paper:

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In vitro evaluation of copper tolerance and accumulation in Populus nigra

Cited by 7 publications

References 26 publications

Biochar improves the morphological, physiological and biochemical properties of white willow seedlings in heavy metal-contaminated soil

Biochar improves the morphological, physiological and biochemical properties of white willow seedlings in heavy metal-contaminated soil

In Vitro Lead Tolerance Testing in White Poplar Genotypes on Acidic Medium

The Potential Role of Cupper and Combined Action with IAA on Tolerance Strategy of Two Broad Bean Cultivars

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