In vitro evaluation of Datura innoxia (thorn-apple) for potential antibacterial activity

Abstract

Various parts of Datura innoxia were examined for potential antibacterial activity by preparing their crude aqueous and organic extracts against Gram-negative bacteria (Escherichia coli and Salmonella typhi) and Gram-positive bacteria (Bacillus cereus, Bacillus subtilis and Staphylococcus aureus). The results of agar well diffusion assay indicated that the pattern of inhibition depends largely upon the plant part, solvent used for extraction and the organism tested. Extracts prepared from leaves were shown to have better effi cacy than stem and root extracts. Organic extracts provided potent antibacterial activity as compared to aqueous extracts. Among all the extracts, methanolic extract was found most active against almost all the bacterial species tested. Gram-positive bacteria were found most sensitive as compared to Gram-negative bacteria. Staphylococcus aureus was signifi cantly inhibited by almost all the extracts even at very low MIC followed by other Gram-positives. For Escherichia coli (a Gram-negative bacterium), the end point was not reached for ethyl acetate extract while it was very high for other extracts. The study promises an interesting future for designing a potentially active antibacterial agent from Datura innoxia.

Phytochemical screening, antioxidants and antimicrobial potential of Lantana camara in different solvents

“…Streptococcus pyogenes, Stephylococcus aureus and Streptococcus pneumoniae, the organisms that causes respiratory and cutaneous infection, as well as Pseudomonas and members of Enterobacteriaceae, causing diarrhea and urinary tract infections and sepsis, are now resistant to virtually all of the known antibiotics [3] . This resistance is largely due to indiscriminate use of antimicrobial drugs commonly used for the treatment of infectious diseases [4] . Furthermore some antibiotics have serious undesirable side effect which limit their application, so there is serious need to develop new antimicrobial agents that are very effective with minimal unwanted side effect.…”

Antimicrobial activity of methanol extract and fractions from <i>Sarcochlamys pulcherrima</i>

“…Antimicrobial activity was tested by agar well diffusion (Kaushik and Goyal, 2008) and agar disc diffusion method (Trakranrungsie et al, 2008). The culture (SDA/ NA) plates were swabbed with 150 mL of the inoculum and loaded with 150 mL of the test extract / fraction into the well of 8 mm diameter, made on the agar plate.…”

Isolation and Characterization of Antidermatophytic Bioactive Molecules from Piper longum L. Leaves

“…Test solutions of extracts/fractions were prepared in dimethyl sulphoxide (DMSO, w/v), filter sterilized (Millipore filter, 0.22 lm) and tested for antidermatophytic activity by agar well diffusion [16] and agar dilution [17] methods. In agar well diffusion method, SDA plate (80 mm dia.)…”