In Vitro Evaluation of Encapsulated Probiotic Bacteria Supplementation to Ruminant Rations

Abstract

The aim of this study was to in-vitro evaluate encapsulated probiotic supplementation to ruminant rationson degradation and fermentation parameters. The ration consisted of 40% alfalfa hay and 60% concentrate feed mixture. Encapsulated and not encapsulated probiotic were supplemented with level of 10 6 cfu/kg of the total dry matter of ration (DM) and compared with encapsulation media (Sodium Alginate, SA) and control (not supplemented ration). DM and OM degradation and total gas production as well as fermentation parameters of the incubated samples were determined after 24 h of fermentation. Significant (P<0.01) increases in in-vitro DM degradability was observed for the experimental ration supplemented with encapsulated or not encapsulated probiotics at levels (10 6 CFU/ kg DM) and SA treatment compared to control ration. Also, significant (P<0.05) improvement in OM degradability was recorded for the ration supplemented with not encapsulated probiotics bacteria compared to the other treatments. Moreover no significant differences were observed between the control ration and the rations supplemented with encapsulated probiotics or SA only, as well as no significant difference was recorded between the ration supplemented with encapsulated probiotics and the ration supplemented with SA only. Probiotics bacteria supple-mentation in the form of not encapsulated probiotic resulted significant increases in in vitro total gas production per sample and per g DM, OM, dDM, NDF and ADF after 24 hours incubation period compared to the other experimental rations (control, encapsulated probiotic and SA). While significant increase in total gas production per g dOM was observed for not encapsulated probiotic compared to encapsulated probiotic only. It could be concluded that, using encapsulated probiotics bacteria had no significant effect on DM degradability and may be induce decrease in gas production and fermentation parameters.

EFFECT OF USING PROBIOTICS ON ARABIAN CAMELS (Camelus dromedarius) FEEDING: AN IN VITRO STUDY.

“…That indicates using probiotic additive with camels can stimulate rumen microorganism which resulted in increasing IVDMD%. Makled et al (2019) found that using probiotic (Lactobacilli isolates with 10 6 cfu/ kg DM) in in vitro evaluation (alfalfa hay and CFM, R:C= 40:60) increased IVDMD from 43.21% to 46.45% in control and probiotic groups, respectively.…”

“…In vitro technique was done according to Tilley and Terry (1963) for one stage only (incubation with buffered rumen liquor without incubation with pepsin enzyme) for determination in vitro dry matter disappearance (IVDMD%) after different incubation periods till 96h and gas production was measured according to Cappellozza et al (2023) and Makled et al (2019).…”

“…• GP/gDM= Total gas production (ml) at 24h / substrate DM (g) GP/gOM = Total gas production (ml) at 24h / substrate OM (g) GP/gNDF = Total gas production (ml) at 24h / substrate NDF (g) GP/gADF = Total gas production (ml) at 24h / substrate ADF (g) According to Makled et al, (2019).…”