In vitro evaluation of excess copper affecting seedlings and their biochemical characteristics in Carthamus tinctorius L. (variety PBNS-12)

Gautam, Swotantra; Anjani, K.

doi:10.1007/s12298-016-0339-1

Cited by 32 publications

(22 citation statements)

References 31 publications

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“…However, in excess in the soil, it becomes phytotoxic to plants, interfering in innumerable physiological processes (Yruela 2013;Küpper and Andresen 2016). The synthesis of photosynthetic pigments, cell membrane permeability, homeostasis, and plant nutrient balance is altered at high Cu concentrations (Adrees et al 2015;Gautam et al 2016). Excess Cu ions in plants cause disturbances in photosynthesis, which can result in oxidative stress, with increasing reactive oxygen species (ROS), reducing carbon fixation, and reducing or inhibiting plant development (Küpper and Andresen 2016).…”

Section: Introductionmentioning

confidence: 99%

Copper Toxicity on Photosynthetic Responses and Root Morphology of Hymenaea courbaril L. (Caesalpinioideae)

Marques

Júnior

Silva

et al. 2018

Water Air Soil Pollut

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Copper (Cu) is a micronutrient essential for plant development. However, in excess, it is toxic to plants and may cause various physiological and morphological changes. The study of the growth of plants exposed to excess Cu is important for the development of phytoremediation programs and for understanding the mechanisms involved in the tolerance of this metal. In this context, the objective of this research was to evaluate the effect of excess copper on photosynthetic responses and root morphology of Hymenaea courbaril L. Biometric measurements, gas exchange, root morphology, and Cu content in tissues and indices (TI and TF) were assessed, involving metal content and biomass. Up to a concentration of 200 mg kg −1 , Cu favored growth, gas exchange, and root morphology of the plants under study. At a higher concentration (800 mg kg −1) in the soil, it affected plant growth and caused a decrease in photosynthetic rate. Biochemical limitations in photosynthesis were observed, as well as lower maximum net photosynthetic rate (A max), respiration rate in the dark (R d), light compensation point (LCP), light saturation point (LSP), and apparent quantum yield (α), when exposed to excess Cu. Root length, surface area, mean diameter, root volume, dry biomass, and specific root length decreased with high Cu concentrations in the soil. Cu was accumulated in the roots as a mechanism of tolerance to the excess of this metal in order to preserve the most metabolically active tissues present in the leaves. At a concentration of 800 mg kg −1 , copper also caused inhibition of the root system. Plants of H. courbaril showed tolerance to excess Cu in the soil and can be indicated for the recovery of areas contaminated with this metal.

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Section: Introductionmentioning

confidence: 99%

Copper Toxicity on Photosynthetic Responses and Root Morphology of Hymenaea courbaril L. (Caesalpinioideae)

Marques

Júnior

Silva

et al. 2018

Water Air Soil Pollut

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“…Dose-response relationships of metal inhibitory eects on seed germination and growth are mainly comparable with those in Arabidopsis [38] and cucumber [43]. Furthermore, failure to germinate or to grow when seeds were exposed to metals may be a result of the failure of subcellular organelles to adjust metal content in cells [44]. Also, the fact that at high Cu level the germination rate was better suggests that the date palm seed coat covering the embryo plays a role in selective penetration of metal into cell sap, as in Arabidopsis seeds exposed to Cu 2þ , Zn 2þ , and Pb 2þ [38].…”

Section: Discussionmentioning

confidence: 78%

“…Also, the fact that at high Cu level the germination rate was better suggests that the date palm seed coat covering the embryo plays a role in selective penetration of metal into cell sap, as in Arabidopsis seeds exposed to Cu 2þ , Zn 2þ , and Pb 2þ [38]. Furthermore, failure to germinate or to grow when seeds were exposed to metals may be a result of the failure of subcellular organelles to adjust metal content in cells [44]. Also, it may be caused by metal interference with cell division [45].…”

Section: Discussionmentioning

confidence: 99%

Copper toxicity and date palm (Phoenix dactylifera) seedling tolerance: Monitoring of related biomarkers

Chaâbene

Hakim

Rorat

et al. 2017

Enviro Toxic and Chemistry

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Date palm (Phoenix dactylifera) seeds were exposed to different copper (Cu) solutions to examine plant stress responses. Low Cu concentrations (0.02 and 0.2 mM) caused an increase of seed germination, whereas higher Cu amounts (2 mM) significantly inhibited seed germination, delayed hypocotyl elongation, increased seedling mortality, and reduced the germination index by more than 90%. Metal-related toxicity symptoms appeared after 15 d of 2 mM of Cu exposure. Biochemical activities such as amylase activity and redox balance elements were examined to study the relationship between external Cu amount and internal plant response. The present study showed that amylolytic activity was dose- and time-dependent. Likewise, H O production increased after exposure to Cu, which was correlated with thiobarbituric acid reactive substance (TBARS) accumulation. Furthermore at low Cu concentrations, superoxide dismutase (SOD) and catalase (CAT) activities increased, suggesting that date palm seed stimulated its metal homeostasis networks. However, the highest cupric ion amounts increased cell oxidant accumulation and reduced enzyme production. Gene expression level measures of P. dactylifera phytochelatin synthase (Pdpcs) and P. dactylifera metallothionein (Pdmt) encoding genes have been carried out to investigate the implication of PdPCS and PdMT proteins in Cu homeostasis and/or its sequestration. Phoenix dactylifera metallothionein induction reached a peak after 30 d of exposure to 0.2 mM of Cu. However, it was down-regulated in plants exposed to higher Cu concentrations. In the same conditions, Pdpcs was overexpressed during 1 mo of exposure before it decreased thereafter. These observations provide a new insight into date palm cell response to Cu, a metal that can be toxic but that is also an essential element. Environ Toxicol Chem 2018;37:797-806. © 2017 SETAC.

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“…Copper (Cu) is an essential micronutrient for plant development, participating in metabolism and being a structural part of some enzymes (Gautam et al, 2016). The concentration of Cu in plant tissues may vary depending on plant species, development stage and environmental factors (Yruela, 2009).…”

Section: Introductionmentioning

confidence: 99%

“…In plants, excess Cu can lead to physiological, biochemical and morpho-anatomical disturbances in plants, causing inhibition of their development (Sánchez-Pardo et al, 2014;Gautam et al, 2016), besides oxidative stress in plants, due to the increase in the production of highly toxic oxygen free radicals that damage lipids and cell membrane proteins, amino acids and nucleic acids (Thounaojam et al, 2012;Moraes et al, 2015), decreasing agricultural productivity (Gao et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

GROWTH AND PHYSIOLOGICAL RESPONSES OF TREE SPECIES (Hymenaea courbaril L., Peltophorum dubium (Spreng.) Taub. and Myroxylon peruiferum L. F.) EXPOSED TO DIFFERENT COPPER CONCENTRATIONS IN THE SOIL

et al. 2018

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-Copper (Cu) is an essential micronutrient for plants. However, when in excess, it becomes phytotoxic. In this context, the objective of this study was to evaluate the growth and physiological responses of tree species exposed to different copper concentrations in the soil. Three experiments were carried out, one for each forest species under study: Myroxylon peruiferum ("Óleo Bálsamo"), Hymenaea courbaril ("Jatobá") and Peltophorum dubium ("Canafístula"), with the same doses of copper (0, 50, 100, 200 and 400 mg kg -1 ). The experimental design was in randomized blocks (DBC), with five copper concentrations and four replicates. The plants were grown on soil substrate packed in 8-dm 3 pots and kept in a greenhouse for 90 days. Biometric measurements, chlorophyll, antioxidant enzymes and copper content in tissues were evaluated. Copper did not influence the vegetative growth of the species studied. The content of chlorophyll "a" was reduced with increasing copper concentrations in the soil. H. courbaril had 56 to 92% copper retained in the roots, and the same behavior was observed for P. dubium (77-91%) and M. peruiferum (19-64%). In the three species studied, there was copper bioaccumulation, mainly in the roots, possibly as a metal tolerance strategy, preserving the most active tissues and the photosynthetic machinery. Cu translocation from roots to shoot was very restricted in all species. This behavior, associated with the increase in the activity of some antioxidant enzymes in plants, may indicate the phytoremediation potential of the studied species.Keywords: Antioxidant enzymes; Bioaccumulation; Heavy metal. CRESCIMENTO E RESPOSTAS FISIOLÓGICAS DAS ESPÉCIES ARBÓREASHymenaea courbaril L., Peltophorum dubium (Spreng.) Taub. e Myroxylon peruiferum L. F. EXPOSTAS A DIFERENTES CONCENTRAÇÕES DE COBRE NO SOLORESUMO -O cobre (Cu) é um micronutriente essencial aos vegetais, mas em excesso torna-se fitotóxico para as plantas. Nesse contexto, o objetivo deste trabalho foi avaliar o crescimento e as respostas fisiológicas de espécies arbóreas expostas a diferentes concentrações de cobre no solo. Foram realizados três experimentos, sendo um para cada espécie florestal em estudo: Myroxylon peruiferum (Óleo Bálsamo), Hymenaea courbaril (Jatobá) e Peltophorum dubium (Canafístula), com as mesmas doses de cobre (0, 50, 100, 200 e 400 mg kg -1 ). O delineamento para os experimentos foi em blocos casualizados (DBC) com cinco concentrações de Cu e quatro repetições. As plantas foram cultivadas em substrato de solo acondicionados em vasos de 8 dm 3 e mantidas em casa-de-vegetação por 90 dias. Foram avaliadas medidas biométricas, clorofila, enzimas

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In vitro evaluation of excess copper affecting seedlings and their biochemical characteristics in Carthamus tinctorius L. (variety PBNS-12)

Cited by 32 publications

References 31 publications

Copper Toxicity on Photosynthetic Responses and Root Morphology of Hymenaea courbaril L. (Caesalpinioideae)

Copper Toxicity on Photosynthetic Responses and Root Morphology of Hymenaea courbaril L. (Caesalpinioideae)

Copper toxicity and date palm (Phoenix dactylifera) seedling tolerance: Monitoring of related biomarkers

GROWTH AND PHYSIOLOGICAL RESPONSES OF TREE SPECIES (Hymenaea courbaril L., Peltophorum dubium (Spreng.) Taub. and Myroxylon peruiferum L. F.) EXPOSED TO DIFFERENT COPPER CONCENTRATIONS IN THE SOIL

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