In vitro evaluation of fungicides and plant extracts against pathogenic fungi of two rice varieties

Abstract

Five pathogenic fungi viz., Alternaria alternata (Fr.) Keissler, Curvularia lunata (Wakker) Boedijn, Drechslera oryzae Breda de Haan (Subramanian and Jain), Fusarium moniliforme Sheldon and Pestalotiopsis guepinii (Desm.) Stay. were isolated from two rice varieties viz., BRRI 29 (Boro) and Pajam (Aman) by Blotter and Tissue planting methods. Ten fungicides i.e. Bavistin 50 WP, Salcox 50 WP, Dithane M-45, Indofil M-45, Tall 25 EC, Ridomil MZ Gold, MC Sulphur 80, Greengel, Hayvit 80 WP and Capvit 50 WP at 100, 200, 300, 400 and 500 ppm were evaluated against the above mentioned five pathogenic fungi. Tall 25 EC completely inhibited the radial growth of the test fungi at all the concentrations except Fusarium moniliforme. Antifungal properties of ethanol extract of Artocarpus heterophyllus Lamk., Tagetes erecta L., Datura metel L., Senna alata (L.) Roxb., Azadirachta indica A. Juss., Citrus medica L., Mangifera indica L., Asparagus racemosus Willd., Nerium indicum Mill. and Allium sativum L. at 5, 10 and 20% concentrations were evaluated against the five test pathogens. All the plant extracts completely inhibited the radial growth of the test fungi at 20% concentration except Asparagus racemosus.

Pathogenic potentiality of fungi associated with the seeds of different cotton (Gossypium hirsutum L.) varieties in Bangladesh

“…Pathogenicity test of the isolated fungi was done following seed inoculation technique (26) . Two hundred seeds of each variety were selected and soaked in distilled water in the beaker for 30 minutes separately and then surface sterilized with 10% Chlorox for 5 minutes.…”

In Vitro Screening of Fungicides and Plant Extracts Against Colletotrichum Gloeosporioides (Penz.) Sacc. the Causal Agent of Anthracnose Disease of Rauwolfia Serpentina (L.) Benth Ex Kurz

“…Sub-culturing of test pathogen: Pure culture of C. gloeosporioides from culture slant was inoculated on PDA plates and incubated for seven days in an incubator at 25 o C. Five mm mycelial block from Petri plates was used for in vitro control of test pathogen. Concentration of fungicides was prepared following Chowdhury et al (2015).…”

In vitro effects of plant extracts and fungicides to control wilt of brinjal (Solanum melongena L.)

“…In recent years, some workers on the fungi toxicity of extracts of various parts of higher plants have indicated the possibility of their exploitation as natural fungi toxicants for controlling plant diseases (12)(13)(14)(15) . Plant metabolites and plant based pesticides appear to be one of the better alternatives as they are known to have minimal environmental impact and danger to consumers in contrast to the synthetic pesticides (16) .…”