In vitro evaluation of fungicides, botanicals and bio-agents against Alternaria alternata causing leaf spot disease of niger

Shingne, Archana W.; Giri, G. K.; Bagade, Ashwini R.

doi:10.22271/chemi.2020.v8.i4ap.10172

Cited by 4 publications

(2 citation statements)

References 5 publications

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“…Peas contain symbiotic bacteria called Rhizobia within root nodules of their Root system (in most of the legume crops). These rhizobium bacteria have the special ability to fix N2 from atmospheric, molecular nitrogen (N2) into Ammonia (NH3) [1][2][3][4][5][6][7][8][9][10].…”

Section: Introductionmentioning

confidence: 99%

Comparative Study of Biofertilizer and Plant Extracts on Growth, Yield and Yield Attributing Traits of Field Pea (Pisum sativum)

Chandhana

Bara

2022

IJECC

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The field experiment was conducted to comparative analyze the effect of biofertilizer and plant extracts on growth, yield and yield attributing traits of field pea (Pisum Sativum L.)” was conducted during Rabi 2021 at field experimental centre, Department of Genetics and Plant Breeding, SHUATS, Prayagraj, (U.P). The soil of experimental plot was sandy loam in texture, nearly neutral in soil reaction (pH 7.1), low in organic carbon (0.36%), available N (171.48 kg/ha), available P (15.2 kg/ha) and available K (232.5 kg/ha). The experiment was laid out in randomized block design with thirteen treatments including control which were replicated thrice. The treatments are as follows, TO- Control, T1, T2, T3, - Rhizobium at the rate of 5%, 8%, 10%, T4, T5, T6 – Vermi wash at the rate of 5%, 8%, 10%, T7, T8, T9 – Neem leaf extract at the rate of 5%, 10%, 15% and T10, T11, T12 – Tulsi leaf extract at the rate of 5%, 10%, 15% respectively. The experiment results revealed that seeds treated with rhizobium at the rate of 8% gave better than other treatments viz, field emergence (87.47%), plant height (95.71 cm), days to 50% flowering (40.60), number of branches per plant (8.87), number of pods per plant (13.53), number of seeds per pod (8.47), seed yield per plant (24.88 g), seed yield per plot (746.50 g), biological yield (33.73 kg/ha), harvest index (73.48%) were recorded significantly higher compared to other treatments.

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Section: Introductionmentioning

confidence: 99%

Comparative Study of Biofertilizer and Plant Extracts on Growth, Yield and Yield Attributing Traits of Field Pea (Pisum sativum)

Chandhana

Bara

2022

IJECC

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“…capitata) by a 10% concentration of fresh turmeric extracted in water. Effect of turmeric on the growth of A. alternata was also reported in niger (Gizotia abyssinica), brinjals (Solanum melongena), and tomatoes (Solanum lycopersicum)(Wongkaew & Sinsiri, 2014;Nagaraju et al, 2020;Shingne et al, 2020). Other species of Alternaria reported to show susceptibility to turmeric are A. porri and A. brassicicola(Suwitchayanon & Kunasakdakul, 2009;Chethana et al, 2012).…”

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confidence: 85%

Activity of extracts from selected Tanzanian spices on major fungal pathogens and blight diseases of Tomato

Lengai

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Tomato (Solanum lycopersicum L.) is affected by many fungal diseases and farmers rely on synthetic pesticides for management. Detrimental effects associated with injudicious use of chemical pesticides have caused a demand for alternative crop protection products. The objective of this study was to evaluate the antifungal activity of selected spices against major fungal pathogens and blight diseases of tomato. An ethnobotanical survey for ginger and turmeric and evaluation of awareness of botanical pesticides use and collection of commonly consumed spices was conducted in Tanzania. Ethanolic extracts from spices were prepared by maceration and tested for antifungal activity in posisoned food bioassay against Phytophthora infestans, Alternaria solani, Fusarium oxysporum f.sp. lycopersici and Pythium spp in vitro, their effect on seed germination and efficacy on severity of early and late blight diseases of tomato in. The most active extracts were fractionated in solvents with varied polarity, analyzed for biochemical composition and tested for fungicidal activity. Results indicated that among the tested spices, clove extract was the most active, inhibiting all the fungal pathogens (100%). Combined effect of the most active extracts showed that clove combined with either ginger, black pepper or turmeric inhibited growth of P. infestans (100%). The activity of solvent fractions was lower compared to the crude ethanolic extracts, except for clove fractioned in n hexane which completely inhibited the growth of P. infestans. Therefore, the most active compounds were better extracted in ethanol. Gas chromatography- mass spectrometry analysis of the clove’s n- hexane fraction showed high abundance of eugenol (74%) which is likely to be responsible for the high antifungal activity. High concentrations of the spice extracts deterred and slowed germination but low concentrations stimulated seed germination of up to 98%. Under field conditions black pepper extract reduced severity of late blight by 40% while clove extract reduced severity of early blight by 35% compared to the untreated control. The findings herein are proof of activity of spice extracts under in vitro and field conditions. This study recommends that the most active extracts be considered for development of a broad botanical fungicide for management of fungal diseases of tomato.

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Helichrysum stoechas (L.) Moench Inflorescence Extract for Tomato Disease Management

et al. 2023

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Helichrysum stoechas is a singular halophyte that has been shown to have anti-inflammatory, antioxidant, and allelopathic properties. In the work presented herein, we have characterized its inflorescences hydromethanolic extract and assessed its antifungal activity for the pre- and postharvest management of tomato crop diseases. Gas chromatography–mass spectrometry characterization of the extract showed that 4-ethenyl-1,3-benzenediol, 2,3-dihydro-benzofuran, quinic acid, 3,5-dihydroxy-6,7,8-trimethoxy-2-phenyl-4H-1-benzopyran-4-one, 1,6-anhydro-β-D-glucopyranose, catechol, scopoletin, and maltol were the main constituents. The co-occurrence of pyranones, benzenediols, and quinic acids as phytoconstituents of H. stoechas extract resulted in promising in vitro minimum inhibitory concentrations of 500, 375, 500, 187.5, 187.5, and 375 μg·mL−1 against mycelia of Alternaria alternata, Colletotrichum coccodes, Fusarium oxysporum f. sp. lycopersici, Rhizoctonia solani, Sclerotinia sclerotiorum, and Verticillium dahliae, respectively. Further, to assess the potential of H. stoechas inflorescence extract for postharvest tomato crop protection, ex situ tests were conducted against C. coccodes, obtaining high protection at a dose of 750 μg·mL−1. Taking into consideration that the demonstrated activity is among the highest reported to date for plant extracts and comparable to that of the synthetic fungicides tested as positive controls, H. stoechas inflorescence extract may be put forward as a promising biorational and may deserve further testing in field-scale studies.

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In vitro evaluation of fungicides, botanicals and bio-agents against Alternaria alternata causing leaf spot disease of niger

Cited by 4 publications

References 5 publications

Comparative Study of Biofertilizer and Plant Extracts on Growth, Yield and Yield Attributing Traits of Field Pea (Pisum sativum)

Comparative Study of Biofertilizer and Plant Extracts on Growth, Yield and Yield Attributing Traits of Field Pea (Pisum sativum)

Activity of extracts from selected Tanzanian spices on major fungal pathogens and blight diseases of Tomato

Helichrysum stoechas (L.) Moench Inflorescence Extract for Tomato Disease Management

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